Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>)
As major elements of the basement membrane, laminins play a significant role in angiogenesis, migration, and adhesion of various cells. <i>Sebastes schlegelii</i> is a marine viviparous teleost of commercial importance. Previous research has reported abundant blood vessels and connective...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Ning Zhao Xueying Wang Tao Wang Xiaojie Xu Qinghua Liu Jun Li |
| author_facet | Ning Zhao Xueying Wang Tao Wang Xiaojie Xu Qinghua Liu Jun Li |
| author_sort | Ning Zhao |
| collection | DOAJ |
| description | As major elements of the basement membrane, laminins play a significant role in angiogenesis, migration, and adhesion of various cells. <i>Sebastes schlegelii</i> is a marine viviparous teleost of commercial importance. Previous research has reported abundant blood vessels and connective tissue in the ovary during gestation. In this study, 14 <i>laminin</i> genes of the α, β, and γ subfamilies from genomic data were identified based on zebrafish and human laminins, distributed on 9 chromosomes in <i>S. schlegelii</i>. Analysis of structural domains showed that coiled-coil regions and EGF domains existed in all <i>laminin</i> genes. Moreover, via qPCR, we found that the expression of <i>laminin</i> genes, including <i>lama4</i>, <i>lama5</i>, <i>lamb4</i>, <i>lamc1</i>, and <i>lamc3</i>, gradually increased from the phase III ovary stage and peaked in the early stage of gestation, especially <i>lama4</i> and <i>lama5</i> which showed dramatically increased expression at the blastula stage. Accordingly, in situ hybridization of <i>lama4</i> was conducted. The results revealed that signals became stronger following the phase IV ovary stage, and the strongest signals were located on the follicular pseudoplacenta at the blastula stage. These results suggest that the high expression of <i>laminin</i> genes, especially <i>lama4</i> after fertilization, may drive cell proliferation, migration, and tissue expansion in the <i>S. schlegelii</i> ovary and ultimately promote follicular pseudoplacenta formation. |
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| spelling | doaj.art-30a7c832f3724c1d9627f34c722a28122023-11-23T16:43:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181052310.3390/ijms231810523Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>)Ning Zhao0Xueying Wang1Tao Wang2Xiaojie Xu3Qinghua Liu4Jun Li5CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaAs major elements of the basement membrane, laminins play a significant role in angiogenesis, migration, and adhesion of various cells. <i>Sebastes schlegelii</i> is a marine viviparous teleost of commercial importance. Previous research has reported abundant blood vessels and connective tissue in the ovary during gestation. In this study, 14 <i>laminin</i> genes of the α, β, and γ subfamilies from genomic data were identified based on zebrafish and human laminins, distributed on 9 chromosomes in <i>S. schlegelii</i>. Analysis of structural domains showed that coiled-coil regions and EGF domains existed in all <i>laminin</i> genes. Moreover, via qPCR, we found that the expression of <i>laminin</i> genes, including <i>lama4</i>, <i>lama5</i>, <i>lamb4</i>, <i>lamc1</i>, and <i>lamc3</i>, gradually increased from the phase III ovary stage and peaked in the early stage of gestation, especially <i>lama4</i> and <i>lama5</i> which showed dramatically increased expression at the blastula stage. Accordingly, in situ hybridization of <i>lama4</i> was conducted. The results revealed that signals became stronger following the phase IV ovary stage, and the strongest signals were located on the follicular pseudoplacenta at the blastula stage. These results suggest that the high expression of <i>laminin</i> genes, especially <i>lama4</i> after fertilization, may drive cell proliferation, migration, and tissue expansion in the <i>S. schlegelii</i> ovary and ultimately promote follicular pseudoplacenta formation.https://www.mdpi.com/1422-0067/23/18/10523<i>Sebastes schlegelii</i>follicular pseudoplacenta<i>laminin</i> genesevolution |
| spellingShingle | Ning Zhao Xueying Wang Tao Wang Xiaojie Xu Qinghua Liu Jun Li Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>) International Journal of Molecular Sciences <i>Sebastes schlegelii</i> follicular pseudoplacenta <i>laminin</i> genes evolution |
| title | Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>) |
| title_full | Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>) |
| title_fullStr | Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>) |
| title_full_unstemmed | Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>) |
| title_short | Genome-Wide Identification of <i>Laminin</i> Family Related to Follicular Pseudoplacenta Development in Black Rockfish (<i>Sebastes schlegelii</i>) |
| title_sort | genome wide identification of i laminin i family related to follicular pseudoplacenta development in black rockfish i sebastes schlegelii i |
| topic | <i>Sebastes schlegelii</i> follicular pseudoplacenta <i>laminin</i> genes evolution |
| url | https://www.mdpi.com/1422-0067/23/18/10523 |
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