Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants
Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phy-tophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-relate...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2015-02-01
|
| Series: | Helminthologia |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/helmin-2015-0014 |
| _version_ | 1797713538309947392 |
|---|---|
| author | Villar-Luna E. García-Espinoza J. A. Goméz-Rodriguez O. Rojas-martínez R. I. Zavaleta-Mejía E. |
| author_facet | Villar-Luna E. García-Espinoza J. A. Goméz-Rodriguez O. Rojas-martínez R. I. Zavaleta-Mejía E. |
| author_sort | Villar-Luna E. |
| collection | DOAJ |
| description | Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phy-tophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by N. aberrans (Na) in CM334 chili roots. Transcripts accumulation of WRKY-a, WRKY1, POX (peroxidase), PR-1 (pathogenesis-related protein 1), and EAS (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334- P. capsici (Pc) interaction. The levels of all studied genes were significantly (P s 0.05) lower (WRKY1, POX and PR-1) or down-regulated (WRKY-a and EAS) in the presence of N. aberrans; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by N. aberrans could be necessary to ensure the successful completion of its life cycle in CM334 chili roots. |
| first_indexed | 2024-03-12T07:38:05Z |
| format | Article |
| id | doaj.art-30a9078149cf4f839e26b667a8e3b6ad |
| institution | Directory Open Access Journal |
| issn | 0440-6605 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T07:38:05Z |
| publishDate | 2015-02-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-30a9078149cf4f839e26b667a8e3b6ad2023-09-02T21:27:24ZengSciendoHelminthologia0440-66051336-90832015-02-01521778210.1515/helmin-2015-0014helmin-2015-0014Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plantsVillar-Luna E.García-Espinoza J. A.Goméz-Rodriguez O.Rojas-martínez R. I.Zavaleta-Mejía E.Capsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phy-tophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by N. aberrans (Na) in CM334 chili roots. Transcripts accumulation of WRKY-a, WRKY1, POX (peroxidase), PR-1 (pathogenesis-related protein 1), and EAS (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334- P. capsici (Pc) interaction. The levels of all studied genes were significantly (P s 0.05) lower (WRKY1, POX and PR-1) or down-regulated (WRKY-a and EAS) in the presence of N. aberrans; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by N. aberrans could be necessary to ensure the successful completion of its life cycle in CM334 chili roots.https://doi.org/10.1515/helmin-2015-0014capsicum annuumqrt-pcrtranscription factorsgene reprogramming |
| spellingShingle | Villar-Luna E. García-Espinoza J. A. Goméz-Rodriguez O. Rojas-martínez R. I. Zavaleta-Mejía E. Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants Helminthologia capsicum annuum qrt-pcr transcription factors gene reprogramming |
| title | Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants |
| title_full | Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants |
| title_fullStr | Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants |
| title_full_unstemmed | Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants |
| title_short | Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants |
| title_sort | defense gene expression in root galls induced by nacobbus aberrans in cm334 chilli plants |
| topic | capsicum annuum qrt-pcr transcription factors gene reprogramming |
| url | https://doi.org/10.1515/helmin-2015-0014 |
| work_keys_str_mv | AT villarlunae defensegeneexpressioninrootgallsinducedbynacobbusaberransincm334chilliplants AT garciaespinozaja defensegeneexpressioninrootgallsinducedbynacobbusaberransincm334chilliplants AT gomezrodriguezo defensegeneexpressioninrootgallsinducedbynacobbusaberransincm334chilliplants AT rojasmartinezri defensegeneexpressioninrootgallsinducedbynacobbusaberransincm334chilliplants AT zavaletamejiae defensegeneexpressioninrootgallsinducedbynacobbusaberransincm334chilliplants |