Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against <italic toggle="yes">Streptococcus pyogenes</italic>

Development of an Opsonophagocytic Killing Assay Using HL-60 Cells for Detection of Functional Antibodies against <italic toggle="yes">Streptococcus pyogenes</italic>

ABSTRACT The clinical development of group A streptococcal (GAS) vaccines will require the implementation of a standardized, high-throughput assay to measure the activity of functional opsonic antibodies in vaccine recipients. In the present study, we adapted and modified the HL-60-based protocol th...

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Bibliographic Details
Main Authors: Sanaz Salehi, Claudia M. Hohn, Thomas A. Penfound, James B. Dale
Format: Article
Language:English
Published: American Society for Microbiology 2018-12-01
Series:mSphere
Subjects:
Streptococcus pyogenes
group A streptococcus
opsonic antibodies
vaccines
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00617-18

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