Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay
Memory B cells (MBCs) are believed to be important for the maintenance of immunity to malaria, and these cells need to be explored in the context of different parasite antigens and their breadth and kinetics after natural infections. However, frequencies of antigen-specific MBCs are low in periphera...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-02-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.619398/full |
| _version_ | 1818589345157742592 |
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| author | Peter Jahnmatz Peter Jahnmatz Christopher Sundling Christopher Sundling Victor Yman Linnea Widman Muhammad Asghar Muhammad Asghar Klara Sondén Klara Sondén Christine Stenström Christian Smedman Francis Ndungu Francis Ndungu Niklas Ahlborg Niklas Ahlborg Anna Färnert Anna Färnert |
| author_facet | Peter Jahnmatz Peter Jahnmatz Christopher Sundling Christopher Sundling Victor Yman Linnea Widman Muhammad Asghar Muhammad Asghar Klara Sondén Klara Sondén Christine Stenström Christian Smedman Francis Ndungu Francis Ndungu Niklas Ahlborg Niklas Ahlborg Anna Färnert Anna Färnert |
| author_sort | Peter Jahnmatz |
| collection | DOAJ |
| description | Memory B cells (MBCs) are believed to be important for the maintenance of immunity to malaria, and these cells need to be explored in the context of different parasite antigens and their breadth and kinetics after natural infections. However, frequencies of antigen-specific MBCs are low in peripheral blood, limiting the number of antigens that can be studied, especially when small blood volumes are available. Here, we developed a multiplexed reversed B-cell FluoroSpot assay capable of simultaneously detecting MBCs specific for the four Plasmodium falciparum blood-stage antigens, MSP-119, MSP-2, MSP-3 and AMA-1. We used the assay to study the kinetics of the MBC response after an acute episode of malaria and up to one year following treatment in travelers returning to Sweden from sub-Saharan Africa. We show that the FluoroSpot assay can detect MBCs to all four merozoite antigens in the same well, and that the breadth and kinetics varied between individuals. We further found that individuals experiencing a primary infection could mount and maintain parasite-specific MBCs to a similar extent as previously exposed adults, already after a single infection. We conclude that the multiplexed B-cell FluoroSpot is a powerful tool for assessing antigen-specific MBC responses to several antigens simultaneously, and that the kinetics of MBC responses against merozoite surface antigens differ over the course of one year. These findings contribute to the understanding of acquisition and maintenance of immune responses to malaria. |
| first_indexed | 2024-12-16T09:39:10Z |
| format | Article |
| id | doaj.art-30aa72b264714114ac722d04144f08c5 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T09:39:10Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-30aa72b264714114ac722d04144f08c52022-12-21T22:36:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-02-011110.3389/fimmu.2020.619398619398Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot AssayPeter Jahnmatz0Peter Jahnmatz1Christopher Sundling2Christopher Sundling3Victor Yman4Linnea Widman5Muhammad Asghar6Muhammad Asghar7Klara Sondén8Klara Sondén9Christine Stenström10Christian Smedman11Francis Ndungu12Francis Ndungu13Niklas Ahlborg14Niklas Ahlborg15Anna Färnert16Anna Färnert17Division of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenMabtech AB, Nacka Strand, SwedenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenDepartment of Infectious Diseases, Karolinska University Hospital, Stockholm, SwedenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenDivision of Biostatistics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, SwedenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenDepartment of Infectious Diseases, Karolinska University Hospital, Stockholm, SwedenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenDepartment of Infectious Diseases, Karolinska University Hospital, Stockholm, SwedenDepartment of Clinical Microbiology, Karolinska University Hospital, Stockholm, SwedenMabtech AB, Nacka Strand, SwedenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenKenya Medical Research Institute (KEMRI)/Wellcome Trust Research Programme, Kilifi, KenyaMabtech AB, Nacka Strand, SwedenDepartment of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, SwedenDivision of Infectious Diseases, Department of Medicine Solna, Karolinska Institutet and Center for Molecular Medicine, Stockholm, SwedenDepartment of Infectious Diseases, Karolinska University Hospital, Stockholm, SwedenMemory B cells (MBCs) are believed to be important for the maintenance of immunity to malaria, and these cells need to be explored in the context of different parasite antigens and their breadth and kinetics after natural infections. However, frequencies of antigen-specific MBCs are low in peripheral blood, limiting the number of antigens that can be studied, especially when small blood volumes are available. Here, we developed a multiplexed reversed B-cell FluoroSpot assay capable of simultaneously detecting MBCs specific for the four Plasmodium falciparum blood-stage antigens, MSP-119, MSP-2, MSP-3 and AMA-1. We used the assay to study the kinetics of the MBC response after an acute episode of malaria and up to one year following treatment in travelers returning to Sweden from sub-Saharan Africa. We show that the FluoroSpot assay can detect MBCs to all four merozoite antigens in the same well, and that the breadth and kinetics varied between individuals. We further found that individuals experiencing a primary infection could mount and maintain parasite-specific MBCs to a similar extent as previously exposed adults, already after a single infection. We conclude that the multiplexed B-cell FluoroSpot is a powerful tool for assessing antigen-specific MBC responses to several antigens simultaneously, and that the kinetics of MBC responses against merozoite surface antigens differ over the course of one year. These findings contribute to the understanding of acquisition and maintenance of immune responses to malaria.https://www.frontiersin.org/articles/10.3389/fimmu.2020.619398/fullantibodymemory B-cellFluoroSpotP. falciparum malariarecombinant proteins |
| spellingShingle | Peter Jahnmatz Peter Jahnmatz Christopher Sundling Christopher Sundling Victor Yman Linnea Widman Muhammad Asghar Muhammad Asghar Klara Sondén Klara Sondén Christine Stenström Christian Smedman Francis Ndungu Francis Ndungu Niklas Ahlborg Niklas Ahlborg Anna Färnert Anna Färnert Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay Frontiers in Immunology antibody memory B-cell FluoroSpot P. falciparum malaria recombinant proteins |
| title | Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay |
| title_full | Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay |
| title_fullStr | Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay |
| title_full_unstemmed | Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay |
| title_short | Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay |
| title_sort | memory b cell responses against merozoite antigens after acute plasmodium falciparum malaria assessed over one year using a novel multiplexed fluorospot assay |
| topic | antibody memory B-cell FluoroSpot P. falciparum malaria recombinant proteins |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.619398/full |
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