Expression profile and association analysis of the porcine DQB1 gene with peripheral blood T lymphocyte subsets
Major histocompatibility complex (MHC) class II molecules play an important role in immunology by presenting antigens to T lymphocytes. As a key member of the MHC class II gene family, the DQB1 gene is involved in interacting with T cells in immune reactions. This study was designed to screen variat...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2015-12-01
|
| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/3864 |
| Summary: | Major histocompatibility complex (MHC) class II molecules play an important role in immunology by presenting antigens to T lymphocytes. As a key member of the MHC class II gene family, the DQB1 gene is involved in interacting with T cells in immune reactions. This study was designed to screen variations in exon 3 of the <em>DQB1</em> gene in Large White, Landrace and Songliao Black pigs, and to investigate the association between <em>DQB1</em> gene polymorphisms and peripheral blood T lymphocyte subsets in Large White samples. In addition, the spatial transcription profile of the <em>DQB1</em> gene was examined, and the effect of gene polymorphisms on its mRNA level was further analyzed. One missense mutation in exon 3 of the <em>DQB1</em> gene was first identified by DNA pool sequencing, and samples were genotyped by using the polymerase chain reactionrestriction fragment length polymorphism method. Statistical analysis indicated that the <em>DQB1</em> genotype was significantly associated with CD4<sup>＋</sup>CD8<sup>－</sup>, CD4<sup>－</sup>CD8<sup>＋</sup> and CD4<sup>＋</sup>/CD8<sup>＋</sup> indexes (P<0.05). Analysis of mRNA distribution showed that <em>DQB1</em> was predominantly expressed in the spleen. However, no significant differences in splenic <em>DQB1</em> mRNA levels of pigs with different genotypes were observed (P>0.05). These results suggest that the <em>DQB1</em> gene may be a promising candidate gene for porcine disease resistance. |
|---|---|
| ISSN: | 1594-4077 1828-051X |