The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr.
In this paper, the composition profiles of bacterial endophytes in wild-growing Amur grape <i>Vitis amurensis</i> Rupr. grown in the south of the Russian Far East were analyzed using both a cultivation-dependent (sowing bacteria) and a cultivation-independent (next generation sequencing,...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/9/1128 |
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| author | Olga A. Aleynova Nikolay N. Nityagovsky Alexandra S. Dubrovina Konstantin V. Kiselev |
| author_facet | Olga A. Aleynova Nikolay N. Nityagovsky Alexandra S. Dubrovina Konstantin V. Kiselev |
| author_sort | Olga A. Aleynova |
| collection | DOAJ |
| description | In this paper, the composition profiles of bacterial endophytes in wild-growing Amur grape <i>Vitis amurensis</i> Rupr. grown in the south of the Russian Far East were analyzed using both a cultivation-dependent (sowing bacteria) and a cultivation-independent (next generation sequencing, NGS) approach. Both methods revealed the prevalent endophytes in <i>V. amurensis</i> were represented by <i>Gammaproteobacteria</i>—40.3–75.8%, <i>Alphaproteobacteria</i>—8.6–18.7%, <i>Actinobacteria</i>—9.2–15.4%, and <i>Bacilli</i>—6.1–6.6%. NGS also showed a large proportion of <i>Bacteroidia</i> (12.2%) and a small proportion of other classes (less than 5.7%). In general, NGS revealed a greater variety of classes and genera in the endophytic bacterial community due to a high number of reads (574,207) in comparison with the number of colonies (933) obtained after the cultivation-dependent method. A comparative analysis performed in this study showed that both wild grape <i>V. amurensis</i> from Russia and domesticated cultivars of <i>V. vinifera</i> from Germany and California (USA) exhibit the same basic composition of endophytic bacteria, while the percentages of major taxa and minor taxa showed some differences depending on the plant organ, grape individuals, environmental conditions, and sampling time. Furthermore, the obtained data revealed that lower temperatures and increased precipitation favored the number and diversity of endophytic bacteria in the wild Amur grape. Thus, this study firstly described and analyzed the biodiversity of endophytic bacteria in wild grapevine <i>V. amurensis</i>. |
| first_indexed | 2024-03-10T03:48:34Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:48:34Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-30acd23ce93f484ca0e115ad9208fd9c2023-11-23T09:01:51ZengMDPI AGPlants2223-77472022-04-01119112810.3390/plants11091128The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr.Olga A. Aleynova0Nikolay N. Nityagovsky1Alexandra S. Dubrovina2Konstantin V. Kiselev3Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690022, RussiaLaboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690022, RussiaLaboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690022, RussiaLaboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690022, RussiaIn this paper, the composition profiles of bacterial endophytes in wild-growing Amur grape <i>Vitis amurensis</i> Rupr. grown in the south of the Russian Far East were analyzed using both a cultivation-dependent (sowing bacteria) and a cultivation-independent (next generation sequencing, NGS) approach. Both methods revealed the prevalent endophytes in <i>V. amurensis</i> were represented by <i>Gammaproteobacteria</i>—40.3–75.8%, <i>Alphaproteobacteria</i>—8.6–18.7%, <i>Actinobacteria</i>—9.2–15.4%, and <i>Bacilli</i>—6.1–6.6%. NGS also showed a large proportion of <i>Bacteroidia</i> (12.2%) and a small proportion of other classes (less than 5.7%). In general, NGS revealed a greater variety of classes and genera in the endophytic bacterial community due to a high number of reads (574,207) in comparison with the number of colonies (933) obtained after the cultivation-dependent method. A comparative analysis performed in this study showed that both wild grape <i>V. amurensis</i> from Russia and domesticated cultivars of <i>V. vinifera</i> from Germany and California (USA) exhibit the same basic composition of endophytic bacteria, while the percentages of major taxa and minor taxa showed some differences depending on the plant organ, grape individuals, environmental conditions, and sampling time. Furthermore, the obtained data revealed that lower temperatures and increased precipitation favored the number and diversity of endophytic bacteria in the wild Amur grape. Thus, this study firstly described and analyzed the biodiversity of endophytic bacteria in wild grapevine <i>V. amurensis</i>.https://www.mdpi.com/2223-7747/11/9/1128bacteriaendophytesgrapemicrobiome<i>Vitis amurensis</i> |
| spellingShingle | Olga A. Aleynova Nikolay N. Nityagovsky Alexandra S. Dubrovina Konstantin V. Kiselev The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr. Plants bacteria endophytes grape microbiome <i>Vitis amurensis</i> |
| title | The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr. |
| title_full | The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr. |
| title_fullStr | The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr. |
| title_full_unstemmed | The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr. |
| title_short | The Biodiversity of Grapevine Bacterial Endophytes of <i>Vitis amurensis</i> Rupr. |
| title_sort | biodiversity of grapevine bacterial endophytes of i vitis amurensis i rupr |
| topic | bacteria endophytes grape microbiome <i>Vitis amurensis</i> |
| url | https://www.mdpi.com/2223-7747/11/9/1128 |
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