Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy
Temporal lobe epilepsy (TLE) is a severe chronic neurological disease caused by abnormal discharge of neurons in the brain and seriously affect the long-term life quality of patients. Currently, new insights into the pathogenesis of TLE are urgently needed to provide more personalized and effective...
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2020.00800/full |
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| author | Yuan-Yuan Dong Min Xia Lin Wang Shuai Cui Qiu-Bo Li Jun-Chen Zhang Shu-Shu Meng Yan-Ke Zhang Qing-Xia Kong |
| author_facet | Yuan-Yuan Dong Min Xia Lin Wang Shuai Cui Qiu-Bo Li Jun-Chen Zhang Shu-Shu Meng Yan-Ke Zhang Qing-Xia Kong |
| author_sort | Yuan-Yuan Dong |
| collection | DOAJ |
| description | Temporal lobe epilepsy (TLE) is a severe chronic neurological disease caused by abnormal discharge of neurons in the brain and seriously affect the long-term life quality of patients. Currently, new insights into the pathogenesis of TLE are urgently needed to provide more personalized and effective therapeutic strategies. Accumulating evidence suggests that sphingosine kinase 1 (SphK1)/sphingosine 1-phosphate receptor 2 (S1PR2) signaling pathway plays a pivotal role in central nervous system (CNS) diseases. However, the precise altered expression of SphK1 and S1PR2 in TLE is remaining obscure. Here, we have confirmed the expression of SphK1 and S1PR2 in the pilocarpine-induced epileptic rat hippocampus and report for the first time the expression of SphK1 and S1PR2 in the temporal cortex of TLE patients. We found an increased expression of SphK1 in the brain from both epileptic rats and TLE patients. Conversely, S1PR2 expression level was markedly decreased. We further investigated the localization of SphK1 and S1PR2 in epileptic brains. Our study showed that both SphK1 and S1PR2 co-localized with activated astrocytes and neurons. Surprisingly, we observed different subcellular localization of SphK1 and S1PR2 in epileptic brain specimens. Taken together, our study suggests that the alteration of the SphK1/S1PR2 signaling axis is closely associated with the course of TLE and provides a new target for the treatment of TLE. |
| first_indexed | 2024-12-13T05:11:37Z |
| format | Article |
| id | doaj.art-30b2aae7289c45638ed5f226c8381972 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-13T05:11:37Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-30b2aae7289c45638ed5f226c83819722022-12-21T23:58:31ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-10-01810.3389/fcell.2020.00800544787Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe EpilepsyYuan-Yuan Dong0Min Xia1Lin Wang2Shuai Cui3Qiu-Bo Li4Jun-Chen Zhang5Shu-Shu Meng6Yan-Ke Zhang7Qing-Xia Kong8Department of Neurology, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Neurology, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Neurology, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Surgery, Weifang Medical University, Weifang, ChinaDepartment of Pediatrics, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Neurosurgery, Affiliated Hospital of Jining Medical University, Jining, ChinaQingdao West Coast New Area Central Hospital, Qingdao, ChinaDepartment of Neurology, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Neurology, Affiliated Hospital of Jining Medical University, Jining, ChinaTemporal lobe epilepsy (TLE) is a severe chronic neurological disease caused by abnormal discharge of neurons in the brain and seriously affect the long-term life quality of patients. Currently, new insights into the pathogenesis of TLE are urgently needed to provide more personalized and effective therapeutic strategies. Accumulating evidence suggests that sphingosine kinase 1 (SphK1)/sphingosine 1-phosphate receptor 2 (S1PR2) signaling pathway plays a pivotal role in central nervous system (CNS) diseases. However, the precise altered expression of SphK1 and S1PR2 in TLE is remaining obscure. Here, we have confirmed the expression of SphK1 and S1PR2 in the pilocarpine-induced epileptic rat hippocampus and report for the first time the expression of SphK1 and S1PR2 in the temporal cortex of TLE patients. We found an increased expression of SphK1 in the brain from both epileptic rats and TLE patients. Conversely, S1PR2 expression level was markedly decreased. We further investigated the localization of SphK1 and S1PR2 in epileptic brains. Our study showed that both SphK1 and S1PR2 co-localized with activated astrocytes and neurons. Surprisingly, we observed different subcellular localization of SphK1 and S1PR2 in epileptic brain specimens. Taken together, our study suggests that the alteration of the SphK1/S1PR2 signaling axis is closely associated with the course of TLE and provides a new target for the treatment of TLE.https://www.frontiersin.org/article/10.3389/fcell.2020.00800/fulltemporal lobe epilepsySphK1S1PR2astrocyteneuron |
| spellingShingle | Yuan-Yuan Dong Min Xia Lin Wang Shuai Cui Qiu-Bo Li Jun-Chen Zhang Shu-Shu Meng Yan-Ke Zhang Qing-Xia Kong Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy Frontiers in Cell and Developmental Biology temporal lobe epilepsy SphK1 S1PR2 astrocyte neuron |
| title | Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy |
| title_full | Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy |
| title_fullStr | Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy |
| title_full_unstemmed | Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy |
| title_short | Spatiotemporal Expression of SphK1 and S1PR2 in the Hippocampus of Pilocarpine Rat Model and the Epileptic Foci of Temporal Lobe Epilepsy |
| title_sort | spatiotemporal expression of sphk1 and s1pr2 in the hippocampus of pilocarpine rat model and the epileptic foci of temporal lobe epilepsy |
| topic | temporal lobe epilepsy SphK1 S1PR2 astrocyte neuron |
| url | https://www.frontiersin.org/article/10.3389/fcell.2020.00800/full |
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