Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate c...

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Main Authors: Pushpendra Awadhiya, Tushar Banerjee, Shridhar Patil
Format: Article
Language:English
Published: Sciendo 2017-09-01
Series:Polish Journal of Microbiology
Subjects:
Online Access:https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4858.pdf
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author Pushpendra Awadhiya
Tushar Banerjee
Shridhar Patil
author_facet Pushpendra Awadhiya
Tushar Banerjee
Shridhar Patil
author_sort Pushpendra Awadhiya
collection DOAJ
description Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.
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spelling doaj.art-30b2c93d1b654158b153f2e2f4fc7ee92022-12-22T03:41:42ZengSciendoPolish Journal of Microbiology1733-13312544-46462017-09-0166310.5604/01.3001.0010.4858Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363Pushpendra Awadhiya0Tushar Banerjee1Shridhar Patil2Applied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, IndiaApplied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, IndiaApplied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, IndiaDelftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4858.pdfDelftia acidovorans MTCC 33634-androstene-317-dione16-dehydropregnenolone acetate and steroid bioconversion
spellingShingle Pushpendra Awadhiya
Tushar Banerjee
Shridhar Patil
Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
Polish Journal of Microbiology
Delftia acidovorans MTCC 3363
4-androstene-3
17-dione
16-dehydropregnenolone acetate and steroid bioconversion
title Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
title_full Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
title_fullStr Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
title_full_unstemmed Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
title_short Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
title_sort bioconversion of 16 dehydropregnenolone acetate to exclusively 4 androstene 3 17 dione by delftia acidovorans mtcc 3363
topic Delftia acidovorans MTCC 3363
4-androstene-3
17-dione
16-dehydropregnenolone acetate and steroid bioconversion
url https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4858.pdf
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