Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363
Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate c...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-09-01
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| Series: | Polish Journal of Microbiology |
| Subjects: | |
| Online Access: | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4858.pdf |
| _version_ | 1828188103354875904 |
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| author | Pushpendra Awadhiya Tushar Banerjee Shridhar Patil |
| author_facet | Pushpendra Awadhiya Tushar Banerjee Shridhar Patil |
| author_sort | Pushpendra Awadhiya |
| collection | DOAJ |
| description | Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD. |
| first_indexed | 2024-04-12T07:46:18Z |
| format | Article |
| id | doaj.art-30b2c93d1b654158b153f2e2f4fc7ee9 |
| institution | Directory Open Access Journal |
| issn | 1733-1331 2544-4646 |
| language | English |
| last_indexed | 2024-04-12T07:46:18Z |
| publishDate | 2017-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-30b2c93d1b654158b153f2e2f4fc7ee92022-12-22T03:41:42ZengSciendoPolish Journal of Microbiology1733-13312544-46462017-09-0166310.5604/01.3001.0010.4858Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363Pushpendra Awadhiya0Tushar Banerjee1Shridhar Patil2Applied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, IndiaApplied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, IndiaApplied Microbiology Laboratory, School of Life Sciences, Devi Ahilya University, Indore, IndiaDelftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, tempera­ture, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4858.pdfDelftia acidovorans MTCC 33634-androstene-317-dione16-dehydropregnenolone acetate and steroid bioconversion |
| spellingShingle | Pushpendra Awadhiya Tushar Banerjee Shridhar Patil Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 Polish Journal of Microbiology Delftia acidovorans MTCC 3363 4-androstene-3 17-dione 16-dehydropregnenolone acetate and steroid bioconversion |
| title | Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 |
| title_full | Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 |
| title_fullStr | Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 |
| title_full_unstemmed | Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 |
| title_short | Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 |
| title_sort | bioconversion of 16 dehydropregnenolone acetate to exclusively 4 androstene 3 17 dione by delftia acidovorans mtcc 3363 |
| topic | Delftia acidovorans MTCC 3363 4-androstene-3 17-dione 16-dehydropregnenolone acetate and steroid bioconversion |
| url | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/3/pdf/10.5604_01.3001.0010.4858.pdf |
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