PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment
Abstract Background The relation between PIM2 and the transcriptional factor NF κβ have been controversial in literature. The significance of PIM2 and NF-κβ genes expression on the incidence of acute leukemia (AML and ALL) and its relevance to the response rate was evaluated. Sixty de novo acute leu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2021-05-01
|
| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43042-021-00162-z |
| _version_ | 1818597439448285184 |
|---|---|
| author | Shymaa Kamal El Din Abed El Rahman Sanaa Sayed Abd Elshafy Mohamed Samra Hala Mohammed Ali Rabab Afifi Mohamed |
| author_facet | Shymaa Kamal El Din Abed El Rahman Sanaa Sayed Abd Elshafy Mohamed Samra Hala Mohammed Ali Rabab Afifi Mohamed |
| author_sort | Shymaa Kamal El Din Abed El Rahman |
| collection | DOAJ |
| description | Abstract Background The relation between PIM2 and the transcriptional factor NF κβ have been controversial in literature. The significance of PIM2 and NF-κβ genes expression on the incidence of acute leukemia (AML and ALL) and its relevance to the response rate was evaluated. Sixty de novo acute leukemia patients were stratified in 2 groups: 30 acute myeloid leukemia (AML) and 30 acute lymphoblastic leukemia (ALL) patients and compared to 30 sex- and age-matched controls. The expression level of PIM2 and NF κβ genes was measured using quantitative real-time polymerase chain reaction (QRT-PCR). The patients were followed with clinical examination and complete blood counts. Results The expression level of PIM2 gene was significantly higher in AML patients (P<0.001) compared to the control group. The mean expression level of NF κβ gene was significantly high in AML and ALL patients compared to the healthy control group (P=0.037 and P<0.001; respectively). The overall survival in AML patients was higher in NF κβ gene low expressers compared to high expressers (P=0.047). The number of AML patients who achieved complete remission was significantly higher in PIM2 gene low expressers in comparison to PIM2 gene high expressers (P=0.042). Conclusion PIM2 and NF κβ genes might have a role in the pathogenesis of acute leukemia, poor overall survival, and failure of response to induction therapy. |
| first_indexed | 2024-12-16T11:47:49Z |
| format | Article |
| id | doaj.art-30b522caaf434a898ffd23d27c05f21d |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-12-16T11:47:49Z |
| publishDate | 2021-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-30b522caaf434a898ffd23d27c05f21d2022-12-21T22:32:47ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412021-05-012211910.1186/s43042-021-00162-zPIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatmentShymaa Kamal El Din Abed El Rahman0Sanaa Sayed Abd Elshafy1Mohamed Samra2Hala Mohammed Ali3Rabab Afifi Mohamed4Clinical and Chemical Pathology Department, Faculty of Medicine, Beni-Suef UniversityClinical and Chemical Pathology Department, Faculty of Medicine, Beni-Suef UniversityDepartment of Medical Oncology, National Cancer Institute, Faculty of Medicine, Cairo UniversityDepartment of Internal Medicine, Faculty of Medicine, Cairo UniversityClinical and Chemical Pathology Department, Faculty of Medicine, Beni-Suef UniversityAbstract Background The relation between PIM2 and the transcriptional factor NF κβ have been controversial in literature. The significance of PIM2 and NF-κβ genes expression on the incidence of acute leukemia (AML and ALL) and its relevance to the response rate was evaluated. Sixty de novo acute leukemia patients were stratified in 2 groups: 30 acute myeloid leukemia (AML) and 30 acute lymphoblastic leukemia (ALL) patients and compared to 30 sex- and age-matched controls. The expression level of PIM2 and NF κβ genes was measured using quantitative real-time polymerase chain reaction (QRT-PCR). The patients were followed with clinical examination and complete blood counts. Results The expression level of PIM2 gene was significantly higher in AML patients (P<0.001) compared to the control group. The mean expression level of NF κβ gene was significantly high in AML and ALL patients compared to the healthy control group (P=0.037 and P<0.001; respectively). The overall survival in AML patients was higher in NF κβ gene low expressers compared to high expressers (P=0.047). The number of AML patients who achieved complete remission was significantly higher in PIM2 gene low expressers in comparison to PIM2 gene high expressers (P=0.042). Conclusion PIM2 and NF κβ genes might have a role in the pathogenesis of acute leukemia, poor overall survival, and failure of response to induction therapy.https://doi.org/10.1186/s43042-021-00162-zNF κβPIM2Acute leukemiaQRT-PCRExpressionResponse |
| spellingShingle | Shymaa Kamal El Din Abed El Rahman Sanaa Sayed Abd Elshafy Mohamed Samra Hala Mohammed Ali Rabab Afifi Mohamed PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment Egyptian Journal of Medical Human Genetics NF κβ PIM2 Acute leukemia QRT-PCR Expression Response |
| title | PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment |
| title_full | PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment |
| title_fullStr | PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment |
| title_full_unstemmed | PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment |
| title_short | PIM2 and NF-κβ gene expression in a sample of AML and ALL Egyptian patients and its relevance to response to treatment |
| title_sort | pim2 and nf κβ gene expression in a sample of aml and all egyptian patients and its relevance to response to treatment |
| topic | NF κβ PIM2 Acute leukemia QRT-PCR Expression Response |
| url | https://doi.org/10.1186/s43042-021-00162-z |
| work_keys_str_mv | AT shymaakamaleldinabedelrahman pim2andnfkbgeneexpressioninasampleofamlandallegyptianpatientsanditsrelevancetoresponsetotreatment AT sanaasayedabdelshafy pim2andnfkbgeneexpressioninasampleofamlandallegyptianpatientsanditsrelevancetoresponsetotreatment AT mohamedsamra pim2andnfkbgeneexpressioninasampleofamlandallegyptianpatientsanditsrelevancetoresponsetotreatment AT halamohammedali pim2andnfkbgeneexpressioninasampleofamlandallegyptianpatientsanditsrelevancetoresponsetotreatment AT rababafifimohamed pim2andnfkbgeneexpressioninasampleofamlandallegyptianpatientsanditsrelevancetoresponsetotreatment |