Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs
This study investigated whether dietary supplementation with magnolol affects growth performance, anti-inflammatory abilities, serum and muscle amino acid profiles, and metabolisms in growing pigs. A total of 42 seventy-days-old growing barrows (Duroc × Landrace × Yorkshire) were randomly allocated...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13952 |
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| author | Yanchen Liu Yuanfei Li Miao Yu Zhimei Tian Jinping Deng Xianyong Ma Yulong Yin |
| author_facet | Yanchen Liu Yuanfei Li Miao Yu Zhimei Tian Jinping Deng Xianyong Ma Yulong Yin |
| author_sort | Yanchen Liu |
| collection | DOAJ |
| description | This study investigated whether dietary supplementation with magnolol affects growth performance, anti-inflammatory abilities, serum and muscle amino acid profiles, and metabolisms in growing pigs. A total of 42 seventy-days-old growing barrows (Duroc × Landrace × Yorkshire) were randomly allocated into two dietary groups: Con, control group (basal diet); and Mag, magnolol group (basal diet supplemented with 400 mg/kg of magnolol). The results revealed that dietary supplementation with magnolol had no effect (<i>p</i> > 0.05) on growth performance. However, magnolol supplementation remarkably increased (<i>p</i> < 0.05) the serum content of albumin, total protein, immunoglobulin G, immunoglobulin M, and interleukin-22. In addition, dietary magnolol supplementation altered the amino acid (AA) profiles in serum and dorsal muscle and particularly increased (<i>p</i> < 0.05) the serum content of arginine and muscle glutamate. Simultaneously, the mRNA expression of genes associated with AA transport in jejunum (<i>SLC38A2</i>, <i>SLC1A5</i>, and <i>SLC7A1</i>) and ileum (<i>SLC1A5</i> and <i>SLC7A1</i>) was higher (<i>p</i> < 0.05) in the Mag group than in the Con group. Additionally, the serum metabolomics analysis showed that the addition of magnolol significantly enhanced (<i>p</i> < 0.05) arginine biosynthesis, as well as <i>D</i>-glutamine and <i>D</i>-glutamate metabolism. Overall, these results suggested that dietary supplementation with magnolol has the potential to improve the accumulation of AAs, protein synthesis, immunity, and body health in growing pigs by increasing intestinal absorption and the transport of AAs. |
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| last_indexed | 2024-03-10T22:40:05Z |
| publishDate | 2023-09-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-30b54a1f403e4110a830673ce5e54fb02023-11-19T11:05:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181395210.3390/ijms241813952Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing PigsYanchen Liu0Yuanfei Li1Miao Yu2Zhimei Tian3Jinping Deng4Xianyong Ma5Yulong Yin6Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaThis study investigated whether dietary supplementation with magnolol affects growth performance, anti-inflammatory abilities, serum and muscle amino acid profiles, and metabolisms in growing pigs. A total of 42 seventy-days-old growing barrows (Duroc × Landrace × Yorkshire) were randomly allocated into two dietary groups: Con, control group (basal diet); and Mag, magnolol group (basal diet supplemented with 400 mg/kg of magnolol). The results revealed that dietary supplementation with magnolol had no effect (<i>p</i> > 0.05) on growth performance. However, magnolol supplementation remarkably increased (<i>p</i> < 0.05) the serum content of albumin, total protein, immunoglobulin G, immunoglobulin M, and interleukin-22. In addition, dietary magnolol supplementation altered the amino acid (AA) profiles in serum and dorsal muscle and particularly increased (<i>p</i> < 0.05) the serum content of arginine and muscle glutamate. Simultaneously, the mRNA expression of genes associated with AA transport in jejunum (<i>SLC38A2</i>, <i>SLC1A5</i>, and <i>SLC7A1</i>) and ileum (<i>SLC1A5</i> and <i>SLC7A1</i>) was higher (<i>p</i> < 0.05) in the Mag group than in the Con group. Additionally, the serum metabolomics analysis showed that the addition of magnolol significantly enhanced (<i>p</i> < 0.05) arginine biosynthesis, as well as <i>D</i>-glutamine and <i>D</i>-glutamate metabolism. Overall, these results suggested that dietary supplementation with magnolol has the potential to improve the accumulation of AAs, protein synthesis, immunity, and body health in growing pigs by increasing intestinal absorption and the transport of AAs.https://www.mdpi.com/1422-0067/24/18/13952magnololpolyphenolamino acidarginine uptakeimmunepigs |
| spellingShingle | Yanchen Liu Yuanfei Li Miao Yu Zhimei Tian Jinping Deng Xianyong Ma Yulong Yin Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs International Journal of Molecular Sciences magnolol polyphenol amino acid arginine uptake immune pigs |
| title | Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs |
| title_full | Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs |
| title_fullStr | Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs |
| title_full_unstemmed | Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs |
| title_short | Magnolol Supplementation Alters Serum Parameters, Immune Homeostasis, Amino Acid Profiles, and Gene Expression of Amino Acid Transporters in Growing Pigs |
| title_sort | magnolol supplementation alters serum parameters immune homeostasis amino acid profiles and gene expression of amino acid transporters in growing pigs |
| topic | magnolol polyphenol amino acid arginine uptake immune pigs |
| url | https://www.mdpi.com/1422-0067/24/18/13952 |
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