Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease
Sugarcane is the most important sugar crop and one of the leading energy-producing crops in the world. Ratoon stunting disease (RSD), caused by the bacterium <i>Leifsonia xyli</i> subsp. <i>xyli</i>, poses a huge threat to ratoon crops, causing a significant yield loss in sug...
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MDPI AG
2024-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/3/448 |
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| author | Xiao-Qiu Zhang Yong-Jian Liang Bao-Qing Zhang Mei-Xin Yan Ze-Ping Wang Dong-Mei Huang Yu-Xin Huang Jing-Chao Lei Xiu-Peng Song Dong-Liang Huang |
| author_facet | Xiao-Qiu Zhang Yong-Jian Liang Bao-Qing Zhang Mei-Xin Yan Ze-Ping Wang Dong-Mei Huang Yu-Xin Huang Jing-Chao Lei Xiu-Peng Song Dong-Liang Huang |
| author_sort | Xiao-Qiu Zhang |
| collection | DOAJ |
| description | Sugarcane is the most important sugar crop and one of the leading energy-producing crops in the world. Ratoon stunting disease (RSD), caused by the bacterium <i>Leifsonia xyli</i> subsp. <i>xyli</i>, poses a huge threat to ratoon crops, causing a significant yield loss in sugarcane. Breeding resistant varieties is considered the most effective and fundamental approach to control RSD in sugarcane. The exploration of resistance genes forms the foundation for breeding resistant varieties through molecular technology. The <i>pglA</i> gene is a pathogenicity gene in <i>L. xyli</i> subsp. <i>xyli</i>, encoding an endopolygalacturonase. In this study, the pglA gene from <i>L. xyli</i> subsp. <i>xyli</i> and related microorganisms was analyzed. Then, a non-toxic, non-autoactivating pglA bait was successfully expressed in yeast cells. Simultaneously the yeast two-hybrid library was generated using RNA from the <i>L. xyli</i> subsp. <i>xyli</i>-infected sugarcane. Screening the library with the pglA bait uncovered proteins that interacted with pglA, primarily associated with ABA pathways and the plant immune system, suggesting that sugarcane employs these pathways to respond to <i>L. xyli</i> subsp. <i>xyli</i>, triggering pathogenicity or resistance. The expression of genes encoding these proteins was also investigated in <i>L. xyli</i> subsp. <i>xyli</i>-infected sugarcane, suggesting multiple layers of regulatory mechanisms in the interaction between sugarcane and <i>L. xyli</i> subsp. <i>xyli</i>. This work promotes the understanding of plant–pathogen interaction and provides target proteins/genes for molecular breeding to improve sugarcane resistance to <i>L. xyli</i> subsp. <i>xyli</i>. |
| first_indexed | 2024-03-08T03:50:31Z |
| format | Article |
| id | doaj.art-30b559c443fd438b91e1355e0f13e0b8 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T03:50:31Z |
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| spelling | doaj.art-30b559c443fd438b91e1355e0f13e0b82024-02-09T15:20:32ZengMDPI AGPlants2223-77472024-02-0113344810.3390/plants13030448Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting DiseaseXiao-Qiu Zhang0Yong-Jian Liang1Bao-Qing Zhang2Mei-Xin Yan3Ze-Ping Wang4Dong-Mei Huang5Yu-Xin Huang6Jing-Chao Lei7Xiu-Peng Song8Dong-Liang Huang9Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi South Subtropical Agricultural Science Research Institute, Chongzuo 532415, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaKey Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaSugarcane is the most important sugar crop and one of the leading energy-producing crops in the world. Ratoon stunting disease (RSD), caused by the bacterium <i>Leifsonia xyli</i> subsp. <i>xyli</i>, poses a huge threat to ratoon crops, causing a significant yield loss in sugarcane. Breeding resistant varieties is considered the most effective and fundamental approach to control RSD in sugarcane. The exploration of resistance genes forms the foundation for breeding resistant varieties through molecular technology. The <i>pglA</i> gene is a pathogenicity gene in <i>L. xyli</i> subsp. <i>xyli</i>, encoding an endopolygalacturonase. In this study, the pglA gene from <i>L. xyli</i> subsp. <i>xyli</i> and related microorganisms was analyzed. Then, a non-toxic, non-autoactivating pglA bait was successfully expressed in yeast cells. Simultaneously the yeast two-hybrid library was generated using RNA from the <i>L. xyli</i> subsp. <i>xyli</i>-infected sugarcane. Screening the library with the pglA bait uncovered proteins that interacted with pglA, primarily associated with ABA pathways and the plant immune system, suggesting that sugarcane employs these pathways to respond to <i>L. xyli</i> subsp. <i>xyli</i>, triggering pathogenicity or resistance. The expression of genes encoding these proteins was also investigated in <i>L. xyli</i> subsp. <i>xyli</i>-infected sugarcane, suggesting multiple layers of regulatory mechanisms in the interaction between sugarcane and <i>L. xyli</i> subsp. <i>xyli</i>. This work promotes the understanding of plant–pathogen interaction and provides target proteins/genes for molecular breeding to improve sugarcane resistance to <i>L. xyli</i> subsp. <i>xyli</i>.https://www.mdpi.com/2223-7747/13/3/448sugarcaneendopolygalacturonasemolecular breeding |
| spellingShingle | Xiao-Qiu Zhang Yong-Jian Liang Bao-Qing Zhang Mei-Xin Yan Ze-Ping Wang Dong-Mei Huang Yu-Xin Huang Jing-Chao Lei Xiu-Peng Song Dong-Liang Huang Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease Plants sugarcane endopolygalacturonase molecular breeding |
| title | Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease |
| title_full | Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease |
| title_fullStr | Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease |
| title_full_unstemmed | Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease |
| title_short | Screening of Sugarcane Proteins Associated with Defense against <i>Leifsonia xyli</i> subsp. <i>xyli</i>, Agent of Ratoon Stunting Disease |
| title_sort | screening of sugarcane proteins associated with defense against i leifsonia xyli i subsp i xyli i agent of ratoon stunting disease |
| topic | sugarcane endopolygalacturonase molecular breeding |
| url | https://www.mdpi.com/2223-7747/13/3/448 |
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