Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers
Introdution: The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells). Methods: In this experimental study, miR-143 was introduced into the SaO...
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| Format: | Article |
| Language: | fas |
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Shahid Sadoughi University of Medical Sciences
2018-10-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-5&slc_lang=en&sid=1 |
| _version_ | 1828420124128837632 |
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| author | fatemeh Barzegari firouzabadi Shahrbanoo oryan Mohammad Hasan Sheikhha Seyed Mehdi Kalantar Ameneh Javed |
| author_facet | fatemeh Barzegari firouzabadi Shahrbanoo oryan Mohammad Hasan Sheikhha Seyed Mehdi Kalantar Ameneh Javed |
| author_sort | fatemeh Barzegari firouzabadi |
| collection | DOAJ |
| description | Introdution: The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells).
Methods: In this experimental study, miR-143 was introduced into the SaOs-2 and Hum 63 cell by the lipofectam 2000 system. Then, the effects of miR-143 on cytotoxicity (72 hours) and expression of Bcl2 gene were measured by the MTT tests and Real Time Pcr protocols, respectively.
Results: The results showed that the lipofectamine 2000 system had no toxicity in both Hum63 and SaOs2 cell lines by itself. SaOs2 cancer cells were more susceptible to miR-143 and Lipo / miR-143 compared to Hum63 cells. The highest reduction in SaOs2 cells was observed after the treatment with Lipo / miR-143 (p <0.05). Furthermore, according to the results, transfection of miR-143 by lipofectamine 2000 into the cell, compared to cell-treated alone by miR-143, significantly reduced Bcl2 expression (p <0.05).
Conclusion: The lipoprotein 2000 system can be effective in absorbing miR-143 into the cells. By targeting various cellular pathways, including the expression level of the Bcl2 gene, miR-143 can be effective in the treatment of bone cancer (Osteosarcoma). |
| first_indexed | 2024-12-10T15:06:25Z |
| format | Article |
| id | doaj.art-30b6f44f4e5442dc802bb60877c98cce |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-10T15:06:25Z |
| publishDate | 2018-10-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-30b6f44f4e5442dc802bb60877c98cce2022-12-22T01:44:02ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332018-10-01266542552Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancersfatemeh Barzegari firouzabadi0Shahrbanoo oryan1Mohammad Hasan Sheikhha2Seyed Mehdi Kalantar3Ameneh Javed4 Kharazmi University Kharazmi University Shahid Sadoughi University of Medical Sciences Shahid Sadoughi University of Medical Sciences Science and Art University Introdution: The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells). Methods: In this experimental study, miR-143 was introduced into the SaOs-2 and Hum 63 cell by the lipofectam 2000 system. Then, the effects of miR-143 on cytotoxicity (72 hours) and expression of Bcl2 gene were measured by the MTT tests and Real Time Pcr protocols, respectively. Results: The results showed that the lipofectamine 2000 system had no toxicity in both Hum63 and SaOs2 cell lines by itself. SaOs2 cancer cells were more susceptible to miR-143 and Lipo / miR-143 compared to Hum63 cells. The highest reduction in SaOs2 cells was observed after the treatment with Lipo / miR-143 (p <0.05). Furthermore, according to the results, transfection of miR-143 by lipofectamine 2000 into the cell, compared to cell-treated alone by miR-143, significantly reduced Bcl2 expression (p <0.05). Conclusion: The lipoprotein 2000 system can be effective in absorbing miR-143 into the cells. By targeting various cellular pathways, including the expression level of the Bcl2 gene, miR-143 can be effective in the treatment of bone cancer (Osteosarcoma).http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-5&slc_lang=en&sid=1miR-143 Lipofectamin Bcl-2 Osteosarcoma. |
| spellingShingle | fatemeh Barzegari firouzabadi Shahrbanoo oryan Mohammad Hasan Sheikhha Seyed Mehdi Kalantar Ameneh Javed Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd miR-143 Lipofectamin Bcl-2 Osteosarcoma. |
| title | Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers |
| title_full | Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers |
| title_fullStr | Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers |
| title_full_unstemmed | Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers |
| title_short | Evaluation of the effect of liposomal miR-143 on cell viability and anti-apoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers |
| title_sort | evaluation of the effect of liposomal mir 143 on cell viability and anti apoptotic gene expression of bcl 2 in human osteosarcoma cells for the treatment of metastatic cancers |
| topic | miR-143 Lipofectamin Bcl-2 Osteosarcoma. |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-303-5&slc_lang=en&sid=1 |
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