Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing
Currently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Recently, MGI Tech launched a series of new sequencers, including the MGISEQ-2000, which promise to deliver high-quality sequencing data faster and at lower prices than Illumina’s se...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korea Genome Organization
2019-09-01
|
| Series: | Genomics & Informatics |
| Subjects: | |
| Online Access: | http://genominfo.org/upload/pdf/gi-2019-17-3-e32.pdf |
| _version_ | 1811193475159293952 |
|---|---|
| author | Sol A Jeon Jong Lyul Park Jong-Hwan Kim Jeong Hwan Kim Yong Sung Kim Jin Cheon Kim Seon-Young Kim |
| author_facet | Sol A Jeon Jong Lyul Park Jong-Hwan Kim Jeong Hwan Kim Yong Sung Kim Jin Cheon Kim Seon-Young Kim |
| author_sort | Sol A Jeon |
| collection | DOAJ |
| description | Currently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Recently, MGI Tech launched a series of new sequencers, including the MGISEQ-2000, which promise to deliver high-quality sequencing data faster and at lower prices than Illumina’s sequencers. In this study, we compared the performance of two major sequencers (MGISEQ-2000 and HiSeq 4000) to test whether the MGISEQ-2000 sequencer delivers high-quality sequence data as suggested. We performed RNA sequencing of four human colon cancer samples with the two platforms, and compared the sequencing quality and expression values. The data produced from the MGISEQ-2000 and HiSeq 4000 showed high concordance, with Pearson correlation coefficients ranging from 0.98 to 0.99. Various quality control (QC) analyses showed that the MGISEQ-2000 data fulfilled the required QC measures. Our study suggests that the performance of the MGISEQ-2000 is comparable to that of the HiSeq 4000 and that the MGISEQ-2000 can be a useful platform for sequencing. |
| first_indexed | 2024-04-12T00:09:09Z |
| format | Article |
| id | doaj.art-30b6f54989d0446db0f347562496744b |
| institution | Directory Open Access Journal |
| issn | 2234-0742 |
| language | English |
| last_indexed | 2024-04-12T00:09:09Z |
| publishDate | 2019-09-01 |
| publisher | Korea Genome Organization |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj.art-30b6f54989d0446db0f347562496744b2022-12-22T03:56:00ZengKorea Genome OrganizationGenomics & Informatics2234-07422019-09-0117310.5808/GI.2019.17.3.e32577Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencingSol A Jeon0Jong Lyul Park1Jong-Hwan Kim2Jeong Hwan Kim3Yong Sung Kim4Jin Cheon Kim5Seon-Young Kim6 Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea Genome Editing Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea Department of Surgery, University of Ulsan College of Medicine, Seoul, Korea Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, KoreaCurrently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Recently, MGI Tech launched a series of new sequencers, including the MGISEQ-2000, which promise to deliver high-quality sequencing data faster and at lower prices than Illumina’s sequencers. In this study, we compared the performance of two major sequencers (MGISEQ-2000 and HiSeq 4000) to test whether the MGISEQ-2000 sequencer delivers high-quality sequence data as suggested. We performed RNA sequencing of four human colon cancer samples with the two platforms, and compared the sequencing quality and expression values. The data produced from the MGISEQ-2000 and HiSeq 4000 showed high concordance, with Pearson correlation coefficients ranging from 0.98 to 0.99. Various quality control (QC) analyses showed that the MGISEQ-2000 data fulfilled the required QC measures. Our study suggests that the performance of the MGISEQ-2000 is comparable to that of the HiSeq 4000 and that the MGISEQ-2000 can be a useful platform for sequencing.http://genominfo.org/upload/pdf/gi-2019-17-3-e32.pdfbenchmarkingHiSeq 4000MGISEQ-2000 |
| spellingShingle | Sol A Jeon Jong Lyul Park Jong-Hwan Kim Jeong Hwan Kim Yong Sung Kim Jin Cheon Kim Seon-Young Kim Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing Genomics & Informatics benchmarking HiSeq 4000 MGISEQ-2000 |
| title | Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing |
| title_full | Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing |
| title_fullStr | Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing |
| title_full_unstemmed | Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing |
| title_short | Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing |
| title_sort | comparison of the mgiseq 2000 and illumina hiseq 4000 sequencing platforms for rna sequencing |
| topic | benchmarking HiSeq 4000 MGISEQ-2000 |
| url | http://genominfo.org/upload/pdf/gi-2019-17-3-e32.pdf |
| work_keys_str_mv | AT solajeon comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing AT jonglyulpark comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing AT jonghwankim comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing AT jeonghwankim comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing AT yongsungkim comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing AT jincheonkim comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing AT seonyoungkim comparisonofthemgiseq2000andilluminahiseq4000sequencingplatformsforrnasequencing |