Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films
Transmucosal drug delivery systems can be an attractive alternative to conventional oral dosage forms such as tablets. There are numerous in vitro methods to estimate the behavior of mucoadhesive dosage forms in vivo. In this work, a tensile test system was used to measure the mucoadhesion of polyvi...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/23/5146 |
| _version_ | 1797462300799533056 |
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| author | Laura Müller Christoph Rosenbaum Julius Krause Werner Weitschies |
| author_facet | Laura Müller Christoph Rosenbaum Julius Krause Werner Weitschies |
| author_sort | Laura Müller |
| collection | DOAJ |
| description | Transmucosal drug delivery systems can be an attractive alternative to conventional oral dosage forms such as tablets. There are numerous in vitro methods to estimate the behavior of mucoadhesive dosage forms in vivo. In this work, a tensile test system was used to measure the mucoadhesion of polyvinyl alcohol films. An in vitro screening of potential influencing variables was performed on biomimetic agar/mucin gels. Among the test device-specific factors, contact time and withdrawal speed were identified as influencing parameters. In addition, influencing factors such as the sample area, which showed a linear relationship in relation to the resulting work, and the liquid addition, which led to an abrupt decrease in adhesion, could be identified. The influence of tissue preparation was investigated in ex vivo experiments on porcine small intestinal tissue. It was found that lower values of F<sub>max</sub> and W<sub>ad</sub> were obtained on processed and fresh tissue than on processed and thawed tissue. Film adhesion on fresh, unprocessed tissue was lowest in most of the animals tested. Comparison of ex vivo measurements on porcine small intestinal tissue with in vitro measurements on agar/mucin gels illustrates the inter- and intra-individual variability of biological tissue. |
| first_indexed | 2024-03-09T17:34:36Z |
| format | Article |
| id | doaj.art-30b9f8e00e3d40ce96774f3ceca3f874 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T17:34:36Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-30b9f8e00e3d40ce96774f3ceca3f8742023-11-24T11:59:08ZengMDPI AGPolymers2073-43602022-11-011423514610.3390/polym14235146Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA FilmsLaura Müller0Christoph Rosenbaum1Julius Krause2Werner Weitschies3Department of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17489 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17489 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17489 Greifswald, GermanyDepartment of Biopharmaceutics and Pharmaceutical Technology, Institute of Pharmacy, University of Greifswald, Felix-Hausdorff-Str. 3, 17489 Greifswald, GermanyTransmucosal drug delivery systems can be an attractive alternative to conventional oral dosage forms such as tablets. There are numerous in vitro methods to estimate the behavior of mucoadhesive dosage forms in vivo. In this work, a tensile test system was used to measure the mucoadhesion of polyvinyl alcohol films. An in vitro screening of potential influencing variables was performed on biomimetic agar/mucin gels. Among the test device-specific factors, contact time and withdrawal speed were identified as influencing parameters. In addition, influencing factors such as the sample area, which showed a linear relationship in relation to the resulting work, and the liquid addition, which led to an abrupt decrease in adhesion, could be identified. The influence of tissue preparation was investigated in ex vivo experiments on porcine small intestinal tissue. It was found that lower values of F<sub>max</sub> and W<sub>ad</sub> were obtained on processed and fresh tissue than on processed and thawed tissue. Film adhesion on fresh, unprocessed tissue was lowest in most of the animals tested. Comparison of ex vivo measurements on porcine small intestinal tissue with in vitro measurements on agar/mucin gels illustrates the inter- and intra-individual variability of biological tissue.https://www.mdpi.com/2073-4360/14/23/5146in vitro–ex vivo correlationmucoadhesive filmstensile studiesporcine small intestine |
| spellingShingle | Laura Müller Christoph Rosenbaum Julius Krause Werner Weitschies Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films Polymers in vitro–ex vivo correlation mucoadhesive films tensile studies porcine small intestine |
| title | Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films |
| title_full | Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films |
| title_fullStr | Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films |
| title_full_unstemmed | Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films |
| title_short | Characterization of an In Vitro/Ex Vivo Mucoadhesiveness Measurement Method of PVA Films |
| title_sort | characterization of an in vitro ex vivo mucoadhesiveness measurement method of pva films |
| topic | in vitro–ex vivo correlation mucoadhesive films tensile studies porcine small intestine |
| url | https://www.mdpi.com/2073-4360/14/23/5146 |
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