Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis
A flow-injection system with sample and reagent addition by the synchronous merging zones approach for calcium determination in milk by flame AAS is proposed. Main parameters were optimized using a factorial design with central point. The optimum conditions were 2.5% (m/v) for La concentration, 8 mL...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual Paulista
2018-09-01
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| Series: | Eclética Química |
| Online Access: | https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/999 |
| _version_ | 1830491904024772608 |
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| author | M. B. Petrovich Volnei Resta Amorim Filho José Anchieta Gomes Neto Mail |
| author_facet | M. B. Petrovich Volnei Resta Amorim Filho José Anchieta Gomes Neto Mail |
| author_sort | M. B. Petrovich |
| collection | DOAJ |
| description | A flow-injection system with sample and reagent addition by the synchronous
merging zones approach for calcium determination in milk by flame AAS is proposed. Main
parameters were optimized using a factorial design with central point. The optimum conditions
were 2.5% (m/v) for La concentration, 8 mL min -1 for the carrier flow-rate, 20 cm for coiled
reactor and 250 ìL for sample volume. Different sample preparation procedures were evaluated
such as dilution in water or acid and microwave-assisted decomposition using concentrated
or diluted acids. The optimized flow system was applied to determine Ca in eleven commercial
milk samples and two standard reference materials diluted in water. Similar calcium levels
were encountered comparing the results obtained by the proposed method (dilution in water)
with those obtained using microwave-oven digestion. Results obtained in two standard reference
materials were in agreement at 95% confidence level with those certified. Recoveries of spiked
samples were in the 93% - 116% range. Relative standard deviation (n = 12) was < 5.4%
and the sample throughput was 150 measurements per hour, corresponding to a consumption
of 250 μL of sample and 6.25 mg La per determination. |
| first_indexed | 2024-12-21T20:19:27Z |
| format | Article |
| id | doaj.art-30bc6988382546fdb41bc91e9fa7fefb |
| institution | Directory Open Access Journal |
| issn | 1678-4618 |
| language | English |
| last_indexed | 2024-12-21T20:19:27Z |
| publishDate | 2018-09-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Eclética Química |
| spelling | doaj.art-30bc6988382546fdb41bc91e9fa7fefb2022-12-21T18:51:31ZengUniversidade Estadual PaulistaEclética Química1678-46182018-09-01323253010.26850/1678-4618eqj.v32.3.2007.p25-30999Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysisM. B. Petrovich0Volnei Resta Amorim Filho1José Anchieta Gomes Neto Mail2Departamento de Química Analítica, Instituto de Química, Universidade Estadual Paulista, P.O. Box 355, 14801-970 Araraquara - SP, BrazilDepartamento de Química Analítica, Instituto de Química, Universidade Estadual Paulista, P.O. Box 355, 14801-970 Araraquara - SP, BrazilDepartamento de Química Analítica, Instituto de Química, Universidade Estadual Paulista, P.O. Box 355, 14801-970 Araraquara - SP, BrazilA flow-injection system with sample and reagent addition by the synchronous merging zones approach for calcium determination in milk by flame AAS is proposed. Main parameters were optimized using a factorial design with central point. The optimum conditions were 2.5% (m/v) for La concentration, 8 mL min -1 for the carrier flow-rate, 20 cm for coiled reactor and 250 ìL for sample volume. Different sample preparation procedures were evaluated such as dilution in water or acid and microwave-assisted decomposition using concentrated or diluted acids. The optimized flow system was applied to determine Ca in eleven commercial milk samples and two standard reference materials diluted in water. Similar calcium levels were encountered comparing the results obtained by the proposed method (dilution in water) with those obtained using microwave-oven digestion. Results obtained in two standard reference materials were in agreement at 95% confidence level with those certified. Recoveries of spiked samples were in the 93% - 116% range. Relative standard deviation (n = 12) was < 5.4% and the sample throughput was 150 measurements per hour, corresponding to a consumption of 250 μL of sample and 6.25 mg La per determination.https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/999 |
| spellingShingle | M. B. Petrovich Volnei Resta Amorim Filho José Anchieta Gomes Neto Mail Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis Eclética Química |
| title | Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis |
| title_full | Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis |
| title_fullStr | Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis |
| title_full_unstemmed | Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis |
| title_short | Direct determination of Calcium in milk by atomic absorption spectrometry using flow-injection analysis |
| title_sort | direct determination of calcium in milk by atomic absorption spectrometry using flow injection analysis |
| url | https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/999 |
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