The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions
Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis, a species belonging to the Mycobacterium tuberculosis complex (MTC) group. Direct bTB diagnosis from suggestive lesions can be performed by nested q-PCR targeting the Rv2807 gene present in the MTC group, as well as the TbD1 gene...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal Rural do Semi-Árido (UFERSA)
2023-03-01
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| Series: | Acta Veterinaria Brasilica |
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| Online Access: | https://periodicos.ufersa.edu.br/acta/article/view/10988 |
| _version_ | 1797867921620336640 |
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| author | Taís Ramalho dos Anjos Maria Júlia Sudária Vinícius Silva Castro Eduardo Eustáquio de Souza Figueiredo Ricardo César Tavares Carvalho |
| author_facet | Taís Ramalho dos Anjos Maria Júlia Sudária Vinícius Silva Castro Eduardo Eustáquio de Souza Figueiredo Ricardo César Tavares Carvalho |
| author_sort | Taís Ramalho dos Anjos |
| collection | DOAJ |
| description | Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis, a species belonging to the Mycobacterium tuberculosis complex (MTC) group. Direct bTB diagnosis from suggestive lesions can be performed by nested q-PCR targeting the Rv2807 gene present in the MTC group, as well as the TbD1 gene, present in M. bovis. In this context, the aim of the present study was to assess the importance of considering positive MTC results for the Rv2807 target gene obtained through the nested real time polymerase chain reaction (nested q-PCR) applied to samples obtained directly from suspected bTB lesions. A total of 174 samples of suggestive bTB caseous lesions were obtained during cattle slaughter in slaughterhouses in the state of Mato Grosso, Brazil. DNA was extracted from the lesions and nested q-PCR was performed to detect both MTC and M. bovis. Both samples positive for the Rv2807 (41/174) and TbD1 (29/174) were submitted to bacterial culturing (23/41), and the DNA of the isolates (23) was extracted and submitted again to nested q-PCR. The Rv2807 gene (MTC) was previously amplified by nested q-PCR directly from the lesions, although the TbD1 gene specific for M. bovis was not amplified previously in four of the successfully isolated samples (4/23), only following isolation, and only the Rv2807 gene was amplified before and after isolation. In conclusion, the target gene Rv2807 (MTC) exhibited higher positivity in the analyzed samples compared to the TbD1 gene (M. bovis). |
| first_indexed | 2024-04-09T23:48:57Z |
| format | Article |
| id | doaj.art-30bfcde56c0d4846a76a6fdc024000c2 |
| institution | Directory Open Access Journal |
| issn | 1981-5484 |
| language | English |
| last_indexed | 2024-04-09T23:48:57Z |
| publishDate | 2023-03-01 |
| publisher | Universidade Federal Rural do Semi-Árido (UFERSA) |
| record_format | Article |
| series | Acta Veterinaria Brasilica |
| spelling | doaj.art-30bfcde56c0d4846a76a6fdc024000c22023-03-17T16:41:32ZengUniversidade Federal Rural do Semi-Árido (UFERSA)Acta Veterinaria Brasilica1981-54842023-03-0116430931210.21708/avb.2022.16.4.10988The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesionsTaís Ramalho dos Anjos0https://orcid.org/0000-0002-4304-1676Maria Júlia Sudária1https://orcid.org/0000-0002-4164-8853Vinícius Silva Castro2https://orcid.org/0000-0002-4746-0237Eduardo Eustáquio de Souza Figueiredo3https://orcid.org/0000-0002-2608-4324Ricardo César Tavares Carvalho4https://orcid.org/0000-0002-0666-7135Programa de Pós-graduação em Biociência Animal - Stricto Sensu, Faculdade de Medicina Veterinária, Universidade de Cuiabá (UNIC), Cuiabá/MT, Brazil.Programa de Pós-graduação em Biociência Animal - Stricto Sensu, Faculdade de Medicina Veterinária, Universidade de Cuiabá (UNIC), Cuiabá/MT, Brazil.Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada, T1K 3M4.Laboratório de Microbiologia Molecular de Alimentos, Faculdade de Nutrição, Universidade Federal de Mato Grosso (UFMT), Cuiabá/MT, Brazil.Programa de Pós-graduação em Biociência Animal - Stricto Sensu, Faculdade de Medicina Veterinária, Universidade de Cuiabá (UNIC), Cuiabá/MT, Brazil.Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis, a species belonging to the Mycobacterium tuberculosis complex (MTC) group. Direct bTB diagnosis from suggestive lesions can be performed by nested q-PCR targeting the Rv2807 gene present in the MTC group, as well as the TbD1 gene, present in M. bovis. In this context, the aim of the present study was to assess the importance of considering positive MTC results for the Rv2807 target gene obtained through the nested real time polymerase chain reaction (nested q-PCR) applied to samples obtained directly from suspected bTB lesions. A total of 174 samples of suggestive bTB caseous lesions were obtained during cattle slaughter in slaughterhouses in the state of Mato Grosso, Brazil. DNA was extracted from the lesions and nested q-PCR was performed to detect both MTC and M. bovis. Both samples positive for the Rv2807 (41/174) and TbD1 (29/174) were submitted to bacterial culturing (23/41), and the DNA of the isolates (23) was extracted and submitted again to nested q-PCR. The Rv2807 gene (MTC) was previously amplified by nested q-PCR directly from the lesions, although the TbD1 gene specific for M. bovis was not amplified previously in four of the successfully isolated samples (4/23), only following isolation, and only the Rv2807 gene was amplified before and after isolation. In conclusion, the target gene Rv2807 (MTC) exhibited higher positivity in the analyzed samples compared to the TbD1 gene (M. bovis).https://periodicos.ufersa.edu.br/acta/article/view/10988nested real time pcrmolecular diagnosismycobacterium tuberculosis complexzoonosis |
| spellingShingle | Taís Ramalho dos Anjos Maria Júlia Sudária Vinícius Silva Castro Eduardo Eustáquio de Souza Figueiredo Ricardo César Tavares Carvalho The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions Acta Veterinaria Brasilica nested real time pcr molecular diagnosis mycobacterium tuberculosis complex zoonosis |
| title | The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions |
| title_full | The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions |
| title_fullStr | The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions |
| title_full_unstemmed | The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions |
| title_short | The Rv2807 target gene: a determining factor to directly detect Mycobacterium bovis from suspected bovine tuberculosis lesions |
| title_sort | rv2807 target gene a determining factor to directly detect mycobacterium bovis from suspected bovine tuberculosis lesions |
| topic | nested real time pcr molecular diagnosis mycobacterium tuberculosis complex zoonosis |
| url | https://periodicos.ufersa.edu.br/acta/article/view/10988 |
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