Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers
Aim: The study investigated the effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal (FCPMO) on the immune responses, antioxidative status, biochemical parameters, and intestinal ecology of broiler chickens. Materials and Methods: Four hundred Lohmann broiler chicken...
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| Format: | Article |
| Language: | English |
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Veterinary World
2020-02-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.13/February-2020/23.pdf |
| _version_ | 1818622066068291584 |
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| author | Sugiharto Sugiharto Endang Widiastuti Isroli Isroli Turrini Yudiarti Tri A. Sartono Hanny I. Wahyuni |
| author_facet | Sugiharto Sugiharto Endang Widiastuti Isroli Isroli Turrini Yudiarti Tri A. Sartono Hanny I. Wahyuni |
| author_sort | Sugiharto Sugiharto |
| collection | DOAJ |
| description | Aim: The study investigated the effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal (FCPMO) on the immune responses, antioxidative status, biochemical parameters, and intestinal ecology of broiler chickens.
Materials and Methods: Four hundred Lohmann broiler chickens were distributed to four groups of diets including CONT (corn-soybean-based feed with no additive), BACI (corn-soybean-based diet supplemented with 0.1% zinc bacitracin), FERM (diet containing 20% FCPMO), and FERB (diet containing 20% FCPMO and added with 0.1% Bacillus subtilis). At days 4, 14, and 19, the chicks were vaccinated using commercial Newcastle disease-infectious bursal disease (ND-IBD), IBD, and ND vaccines, respectively. At day 35, blood was sampled and digesta was obtained from the ileum and caecum. Furthermore, the duodenal segment was obtained.
Results: The BACI, FERM, and FERB groups had higher (p<0.05) serum superoxide dismutase activity than control. The malondialdehyde was lower (p=0.07) in BACI, FERM, and FERB than that in CONT. The BACI and FERM had lower (p<0.05) leukocytes and lymphocytes than CONT. The hemoglobin, erythrocytes, and hematocrit were lower (p<0.05) in BACI and FERM than those in CONT and FERB. Serum total triglyceride was lower (p<0.05) in FERM and FERB than that in CONT. The FERM and FERB had higher (p<0.05) albumin levels. Serum globulin level was lower (p<0.05) in FERB than that in BACI, but did not differ from that in CONT and FERM. The numbers of coliform, lactose-negative enterobacteria and enterobacteria were lower (p<0.05) in FERB than that in other treatment groups. Crypt depth (CD) was higher (p<0.05) in FERM, while the villi height to CD ratio was lower (p<0.05) in FERM than that in CONT and FERB. The treatments showed no effect (p>0.05) on cecal volatile fatty acids production.
Conclusion: Feeding FCPMO improved immune responses, antioxidative status, and physiological conditions, but had less effect on the intestinal ecology of broilers. |
| first_indexed | 2024-12-16T18:19:15Z |
| format | Article |
| id | doaj.art-30c14d8a855e480aa35a963cea33a1cf |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T18:19:15Z |
| publishDate | 2020-02-01 |
| publisher | Veterinary World |
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| series | Veterinary World |
| spelling | doaj.art-30c14d8a855e480aa35a963cea33a1cf2022-12-21T22:21:36ZengVeterinary WorldVeterinary World0972-89882231-09162020-02-0113239239910.14202/vetworld.2020.392-399Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilersSugiharto Sugiharto0Endang Widiastuti1Isroli Isroli2Turrini Yudiarti3Tri A. Sartono4Hanny I. Wahyuni5Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.Department of Animal Science, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Central Java, Indonesia.Aim: The study investigated the effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal (FCPMO) on the immune responses, antioxidative status, biochemical parameters, and intestinal ecology of broiler chickens. Materials and Methods: Four hundred Lohmann broiler chickens were distributed to four groups of diets including CONT (corn-soybean-based feed with no additive), BACI (corn-soybean-based diet supplemented with 0.1% zinc bacitracin), FERM (diet containing 20% FCPMO), and FERB (diet containing 20% FCPMO and added with 0.1% Bacillus subtilis). At days 4, 14, and 19, the chicks were vaccinated using commercial Newcastle disease-infectious bursal disease (ND-IBD), IBD, and ND vaccines, respectively. At day 35, blood was sampled and digesta was obtained from the ileum and caecum. Furthermore, the duodenal segment was obtained. Results: The BACI, FERM, and FERB groups had higher (p<0.05) serum superoxide dismutase activity than control. The malondialdehyde was lower (p=0.07) in BACI, FERM, and FERB than that in CONT. The BACI and FERM had lower (p<0.05) leukocytes and lymphocytes than CONT. The hemoglobin, erythrocytes, and hematocrit were lower (p<0.05) in BACI and FERM than those in CONT and FERB. Serum total triglyceride was lower (p<0.05) in FERM and FERB than that in CONT. The FERM and FERB had higher (p<0.05) albumin levels. Serum globulin level was lower (p<0.05) in FERB than that in BACI, but did not differ from that in CONT and FERM. The numbers of coliform, lactose-negative enterobacteria and enterobacteria were lower (p<0.05) in FERB than that in other treatment groups. Crypt depth (CD) was higher (p<0.05) in FERM, while the villi height to CD ratio was lower (p<0.05) in FERM than that in CONT and FERB. The treatments showed no effect (p>0.05) on cecal volatile fatty acids production. Conclusion: Feeding FCPMO improved immune responses, antioxidative status, and physiological conditions, but had less effect on the intestinal ecology of broilers.http://www.veterinaryworld.org/Vol.13/February-2020/23.pdfantioxidantbroilerfermented feedhealth |
| spellingShingle | Sugiharto Sugiharto Endang Widiastuti Isroli Isroli Turrini Yudiarti Tri A. Sartono Hanny I. Wahyuni Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers Veterinary World antioxidant broiler fermented feed health |
| title | Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers |
| title_full | Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers |
| title_fullStr | Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers |
| title_full_unstemmed | Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers |
| title_short | Effect of feeding fermented mixture of cassava pulp and Moringa oleifera leaf meal on immune responses, antioxidative status, biochemistry indices, and intestinal ecology of broilers |
| title_sort | effect of feeding fermented mixture of cassava pulp and moringa oleifera leaf meal on immune responses antioxidative status biochemistry indices and intestinal ecology of broilers |
| topic | antioxidant broiler fermented feed health |
| url | http://www.veterinaryworld.org/Vol.13/February-2020/23.pdf |
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