Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
Summary: Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated c...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672100188X |
| _version_ | 1819098423669817344 |
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| author | Pawel Bieniasz-Krzywiec Rosa Martín-Pérez Carla Riera-Domingo Massimiliano Mazzone |
| author_facet | Pawel Bieniasz-Krzywiec Rosa Martín-Pérez Carla Riera-Domingo Massimiliano Mazzone |
| author_sort | Pawel Bieniasz-Krzywiec |
| collection | DOAJ |
| description | Summary: Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated cell sorting (FACS) of TAMs from murine orthotopic 4T1 breast tumors. This includes steps to separate PoEMs (podoplanin-expressing macrophages) and non-PoEMs (podoplanin-negative macrophages). Our FACS separation approach could also be used for other tumor types with TAM infiltration.For complete details on the use and execution of this protocol, please refer to Bieniasz-Krzywiec et al. (2019). |
| first_indexed | 2024-12-22T00:30:45Z |
| format | Article |
| id | doaj.art-30c3dd3b2bf94178ade65856ffb94ee6 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T00:30:45Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-30c3dd3b2bf94178ade65856ffb94ee62022-12-21T18:44:57ZengElsevierSTAR Protocols2666-16672021-06-0122100481Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumorsPawel Bieniasz-Krzywiec0Rosa Martín-Pérez1Carla Riera-Domingo2Massimiliano Mazzone3Laboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, VIB, Leuven B3000, Belgium; Laboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, Department of Oncology, KU Leuven, Leuven B3000, BelgiumLaboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, VIB, Leuven B3000, Belgium; Laboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, Department of Oncology, KU Leuven, Leuven B3000, BelgiumLaboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, VIB, Leuven B3000, Belgium; Laboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, Department of Oncology, KU Leuven, Leuven B3000, BelgiumLaboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, VIB, Leuven B3000, Belgium; Laboratory of Tumor Inflammation and Angiogenesis, Center for Cancer Biology, Department of Oncology, KU Leuven, Leuven B3000, Belgium; Corresponding authorSummary: Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated cell sorting (FACS) of TAMs from murine orthotopic 4T1 breast tumors. This includes steps to separate PoEMs (podoplanin-expressing macrophages) and non-PoEMs (podoplanin-negative macrophages). Our FACS separation approach could also be used for other tumor types with TAM infiltration.For complete details on the use and execution of this protocol, please refer to Bieniasz-Krzywiec et al. (2019).http://www.sciencedirect.com/science/article/pii/S266616672100188XCancerCell isolationFlow Cytometry/Mass CytometryImmunologyModel Organisms |
| spellingShingle | Pawel Bieniasz-Krzywiec Rosa Martín-Pérez Carla Riera-Domingo Massimiliano Mazzone Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors STAR Protocols Cancer Cell isolation Flow Cytometry/Mass Cytometry Immunology Model Organisms |
| title | Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors |
| title_full | Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors |
| title_fullStr | Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors |
| title_full_unstemmed | Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors |
| title_short | Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors |
| title_sort | isolation and separation of murine tumor associated macrophages tams subpopulations from orthotopic 4t1 breast tumors |
| topic | Cancer Cell isolation Flow Cytometry/Mass Cytometry Immunology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S266616672100188X |
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