Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors

Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors

Summary: Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated c...

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Bibliographic Details
Main Authors: Pawel Bieniasz-Krzywiec, Rosa Martín-Pérez, Carla Riera-Domingo, Massimiliano Mazzone
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cancer
Cell isolation
Flow Cytometry/Mass Cytometry
Immunology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100188X

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