The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique
(1) Background: the Miombo woodlands comprise the most important vegetation from southern Africa and are dominated by tree legumes with an ecology highly driven by fires. Here, we report on the characterization of bacterial communities from the rhizosphere of <i>Brachystegia boehmii</i>...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1562 |
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| author | Ivete Sandra Alberto Maquia Paula Fareleira Isabel Videira e. Castro Ricardo Soares Denise R. A. Brito Aires Afonso Mbanze Aniceto Chaúque Cristina Máguas Obinna T. Ezeokoli Natasha Sofia Ribeiro Isabel Marques Ana I. Ribeiro-Barros |
| author_facet | Ivete Sandra Alberto Maquia Paula Fareleira Isabel Videira e. Castro Ricardo Soares Denise R. A. Brito Aires Afonso Mbanze Aniceto Chaúque Cristina Máguas Obinna T. Ezeokoli Natasha Sofia Ribeiro Isabel Marques Ana I. Ribeiro-Barros |
| author_sort | Ivete Sandra Alberto Maquia |
| collection | DOAJ |
| description | (1) Background: the Miombo woodlands comprise the most important vegetation from southern Africa and are dominated by tree legumes with an ecology highly driven by fires. Here, we report on the characterization of bacterial communities from the rhizosphere of <i>Brachystegia boehmii</i> in different soil types from areas subjected to different regimes. (2) Methods: bacterial communities were identified through Illumina MiSeq sequencing (16S rRNA). <i>Vigna unguiculata</i> was used as a trap to capture nitrogen-fixing bacteria and culture-dependent methods in selective media were used to isolate plant growth promoting bacteria (PGPB). PGP traits were analysed and molecular taxonomy of the purified isolates was performed. (3) Results: Bacterial communities in the Miombo rhizosphere are highly diverse and driven by soil type and fire regime. Independent of the soil or fire regime, the functional diversity was high, and the different consortia maintained the general functions. A diverse pool of diazotrophs was isolated, and included symbiotic (e.g., <i>Mesorhizobium</i> sp., <i>Neorhizobium galegae</i>, <i>Rhizobium</i> sp., and <i>Ensifer</i> adhaerens), and non-symbiotic (e.g., <i>Agrobacterium</i> sp., <i>Burkholderia</i> sp., <i>Cohnella</i> sp., <i>Microvirga</i> sp., <i>Pseudomonas</i> sp., and <i>Stenotrophomonas</i> sp.) bacteria. Several isolates presented cumulative PGP traits. (4) Conclusions: Although the dynamics of bacterial communities from the Miombo rhizosphere is driven by fire, the maintenance of high levels of diversity and functions remain unchanged, constituting a source of promising bacteria in terms of plant-beneficial activities such as mobilization and acquisition of nutrients, mitigation of abiotic stress, and modulation of plant hormone levels. |
| first_indexed | 2024-03-10T08:34:57Z |
| format | Article |
| id | doaj.art-30cc6f54c36e44438eae95c4542d93f8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:57Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-30cc6f54c36e44438eae95c4542d93f82023-11-22T08:45:33ZengMDPI AGMicroorganisms2076-26072021-07-0198156210.3390/microorganisms9081562The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, MozambiqueIvete Sandra Alberto Maquia0Paula Fareleira1Isabel Videira e. Castro2Ricardo Soares3Denise R. A. Brito4Aires Afonso Mbanze5Aniceto Chaúque6Cristina Máguas7Obinna T. Ezeokoli8Natasha Sofia Ribeiro9Isabel Marques10Ana I. Ribeiro-Barros11Forest Research Center, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, PortugalNational Institute of Agricultural and Veterinary Research, I.P. (INIAV, I.P), 2780-157 Oeiras, PortugalNational Institute of Agricultural and Veterinary Research, I.P. (INIAV, I.P), 2780-157 Oeiras, PortugalNational Institute of Agricultural and Veterinary Research, I.P. (INIAV, I.P), 2780-157 Oeiras, PortugalBiotechnology Center, Eduardo Mondlane University, Maputo 3453, MozambiqueFaculty of Agrarian Science, Lúrio University, Niassa 3302, MozambiqueFaculty of Agronomy and Forest Engineering, Eduardo Mondlane University, Maputo 3453, MozambiqueCentre for Ecology, Evolution and Environmental Changes (cE3c), Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, PortugalDepartment of Microbiology and Biochemistry, University of the Free State, Bloemfontein 9300, South AfricaFaculty of Agronomy and Forest Engineering, Eduardo Mondlane University, Maputo 3453, MozambiqueForest Research Center, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, PortugalForest Research Center, School of Agriculture, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal(1) Background: the Miombo woodlands comprise the most important vegetation from southern Africa and are dominated by tree legumes with an ecology highly driven by fires. Here, we report on the characterization of bacterial communities from the rhizosphere of <i>Brachystegia boehmii</i> in different soil types from areas subjected to different regimes. (2) Methods: bacterial communities were identified through Illumina MiSeq sequencing (16S rRNA). <i>Vigna unguiculata</i> was used as a trap to capture nitrogen-fixing bacteria and culture-dependent methods in selective media were used to isolate plant growth promoting bacteria (PGPB). PGP traits were analysed and molecular taxonomy of the purified isolates was performed. (3) Results: Bacterial communities in the Miombo rhizosphere are highly diverse and driven by soil type and fire regime. Independent of the soil or fire regime, the functional diversity was high, and the different consortia maintained the general functions. A diverse pool of diazotrophs was isolated, and included symbiotic (e.g., <i>Mesorhizobium</i> sp., <i>Neorhizobium galegae</i>, <i>Rhizobium</i> sp., and <i>Ensifer</i> adhaerens), and non-symbiotic (e.g., <i>Agrobacterium</i> sp., <i>Burkholderia</i> sp., <i>Cohnella</i> sp., <i>Microvirga</i> sp., <i>Pseudomonas</i> sp., and <i>Stenotrophomonas</i> sp.) bacteria. Several isolates presented cumulative PGP traits. (4) Conclusions: Although the dynamics of bacterial communities from the Miombo rhizosphere is driven by fire, the maintenance of high levels of diversity and functions remain unchanged, constituting a source of promising bacteria in terms of plant-beneficial activities such as mobilization and acquisition of nutrients, mitigation of abiotic stress, and modulation of plant hormone levels.https://www.mdpi.com/2076-2607/9/8/1562Miombofire16SrRNArhizosphereplant growth promoting bacteria<i>Brachystegia boehmii</i> |
| spellingShingle | Ivete Sandra Alberto Maquia Paula Fareleira Isabel Videira e. Castro Ricardo Soares Denise R. A. Brito Aires Afonso Mbanze Aniceto Chaúque Cristina Máguas Obinna T. Ezeokoli Natasha Sofia Ribeiro Isabel Marques Ana I. Ribeiro-Barros The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique Microorganisms Miombo fire 16SrRNA rhizosphere plant growth promoting bacteria <i>Brachystegia boehmii</i> |
| title | The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique |
| title_full | The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique |
| title_fullStr | The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique |
| title_full_unstemmed | The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique |
| title_short | The Nexus between Fire and Soil Bacterial Diversity in the African Miombo Woodlands of Niassa Special Reserve, Mozambique |
| title_sort | nexus between fire and soil bacterial diversity in the african miombo woodlands of niassa special reserve mozambique |
| topic | Miombo fire 16SrRNA rhizosphere plant growth promoting bacteria <i>Brachystegia boehmii</i> |
| url | https://www.mdpi.com/2076-2607/9/8/1562 |
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