Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis
<b> </b>Basic helix-loop-helix (BHLH) transcription factors differentiated embryonic chondrocyte gene 1 (DEC1) and gene 2 (DEC2) regulate circadian rhythms, apoptosis, epithelial mesenchymal transition (EMT), invasions and metastases in various kinds of cancer. The stem cell markers SOX2...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Clocks & Sleep |
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| Online Access: | https://www.mdpi.com/2624-5175/2/1/4 |
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| author | Fuyuki Sato Ujjal K. Bhawal Nao Sugiyama Shoko Osaki Kosuke Oikawa Yasuteru Muragaki |
| author_facet | Fuyuki Sato Ujjal K. Bhawal Nao Sugiyama Shoko Osaki Kosuke Oikawa Yasuteru Muragaki |
| author_sort | Fuyuki Sato |
| collection | DOAJ |
| description | <b> </b>Basic helix-loop-helix (BHLH) transcription factors differentiated embryonic chondrocyte gene 1 (DEC1) and gene 2 (DEC2) regulate circadian rhythms, apoptosis, epithelial mesenchymal transition (EMT), invasions and metastases in various kinds of cancer. The stem cell markers SOX2 and c-MYC are involved in the regulation of apoptosis and poor prognosis. In cervical cancer, however, their roles are not well elucidated yet. To determine the function of these genes in human cervical cancer, we examined the expression of DEC1, DEC2, SOX2 and c-MYC in human cervical cancer tissues. In immunohistochemistry, they were strongly expressed in cancer cells compared with in non-cancerous cells. Notably, the strong rate of DEC1 and SOX2 expressions were over 80% among 20 cases. We further examined the roles of DEC1 and DEC2 in apoptosis. Human cervical cancer HeLa and SiHa cells were treated with cisplatin—HeLa cells were sensitive to apoptosis, but SiHa cells were resistant. DEC1 expression decreased in the cisplatin-treated HeLa cells, but had little effect on SiHa cells. Combination treatment of DEC1 overexpression and cisplatin inhibited apoptosis and affected SOX2 and c-MYC expressions in HeLa cells. Meanwhile, DEC2 overexpression had little effect on apoptosis and on SOX2 and c-MYC expressions. We conclude that DEC1 has anti-apoptotic effects and regulates SOX2 and c-MYC expressions on apoptosis. |
| first_indexed | 2024-04-12T05:31:07Z |
| format | Article |
| id | doaj.art-30d0bb6469c24a709a54299e9d66694f |
| institution | Directory Open Access Journal |
| issn | 2624-5175 |
| language | English |
| last_indexed | 2024-04-12T05:31:07Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Clocks & Sleep |
| spelling | doaj.art-30d0bb6469c24a709a54299e9d66694f2022-12-22T03:46:04ZengMDPI AGClocks & Sleep2624-51752020-01-0121263810.3390/clockssleep2010004clockssleep2010004Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and ApoptosisFuyuki Sato0Ujjal K. Bhawal1Nao Sugiyama2Shoko Osaki3Kosuke Oikawa4Yasuteru Muragaki5Department of Pathology, Wakayama Medical University School of Medicine, Wakayama 641-8509, JapanDepartment of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, JapanDepartment of Pathology, Wakayama Medical University School of Medicine, Wakayama 641-8509, JapanDepartment of Pathology, Wakayama Medical University School of Medicine, Wakayama 641-8509, JapanDepartment of Pathology, Wakayama Medical University School of Medicine, Wakayama 641-8509, JapanDepartment of Pathology, Wakayama Medical University School of Medicine, Wakayama 641-8509, Japan<b> </b>Basic helix-loop-helix (BHLH) transcription factors differentiated embryonic chondrocyte gene 1 (DEC1) and gene 2 (DEC2) regulate circadian rhythms, apoptosis, epithelial mesenchymal transition (EMT), invasions and metastases in various kinds of cancer. The stem cell markers SOX2 and c-MYC are involved in the regulation of apoptosis and poor prognosis. In cervical cancer, however, their roles are not well elucidated yet. To determine the function of these genes in human cervical cancer, we examined the expression of DEC1, DEC2, SOX2 and c-MYC in human cervical cancer tissues. In immunohistochemistry, they were strongly expressed in cancer cells compared with in non-cancerous cells. Notably, the strong rate of DEC1 and SOX2 expressions were over 80% among 20 cases. We further examined the roles of DEC1 and DEC2 in apoptosis. Human cervical cancer HeLa and SiHa cells were treated with cisplatin—HeLa cells were sensitive to apoptosis, but SiHa cells were resistant. DEC1 expression decreased in the cisplatin-treated HeLa cells, but had little effect on SiHa cells. Combination treatment of DEC1 overexpression and cisplatin inhibited apoptosis and affected SOX2 and c-MYC expressions in HeLa cells. Meanwhile, DEC2 overexpression had little effect on apoptosis and on SOX2 and c-MYC expressions. We conclude that DEC1 has anti-apoptotic effects and regulates SOX2 and c-MYC expressions on apoptosis.https://www.mdpi.com/2624-5175/2/1/4dec1sox2c-mycimmunohistochemistrycervical cancer |
| spellingShingle | Fuyuki Sato Ujjal K. Bhawal Nao Sugiyama Shoko Osaki Kosuke Oikawa Yasuteru Muragaki Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis Clocks & Sleep dec1 sox2 c-myc immunohistochemistry cervical cancer |
| title | Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis |
| title_full | Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis |
| title_fullStr | Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis |
| title_full_unstemmed | Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis |
| title_short | Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis |
| title_sort | potential role of dec1 in cervical cancer cells involving overexpression and apoptosis |
| topic | dec1 sox2 c-myc immunohistochemistry cervical cancer |
| url | https://www.mdpi.com/2624-5175/2/1/4 |
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