Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism
ObjectiveThe aim of this study was to detect botulinum toxin and Clostridium botulinum （C.botulinum） in 30 batches of infant formula milk powder obtained from an enterprise and to analyze the whole genome of the strain of C. botulinum type B isolated from the samples.MethodsThe samples were subjecte...
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| Format: | Article |
| Language: | zho |
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The Editorial Office of Chinese Journal of Food Hygiene
2023-10-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | https://www.zgspws.com/zgspwszz/article/abstract/202310011?st=article_issue |
| _version_ | 1827346686064721920 |
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| author | LUO Haipeng QU Hongren DING Bo REN Xiu ZHAO Linna BAI Jichao WANG Yaping LIN Lan CUI Shenghui |
| author_facet | LUO Haipeng QU Hongren DING Bo REN Xiu ZHAO Linna BAI Jichao WANG Yaping LIN Lan CUI Shenghui |
| author_sort | LUO Haipeng |
| collection | DOAJ |
| description | ObjectiveThe aim of this study was to detect botulinum toxin and Clostridium botulinum （C.botulinum） in 30 batches of infant formula milk powder obtained from an enterprise and to analyze the whole genome of the strain of C. botulinum type B isolated from the samples.MethodsThe samples were subjected to C. botulinum isolation and botulinum toxin typing experiments according to the “National standard for food safety microbiological examination of C. botulinum and botulinum toxin” （GB 4789.12—2016）. The whole genomes of the isolated strains were sequenced and their genetic characteristics were analyzed.ResultsBotulinum toxin was not detected in any of the 30 batches of samples. After intraperitoneal injection of the enrichment solution into mice， four batches of milk powder samples induced symptoms of botulism， but C. botulinum was isolated from only one batch of milk powder samples. Genome sequencing revealed that the bacterium was C. botulinum group Ⅰ， type B， the toxin gene cluster was type Ha， and the toxin gene was subtype B2.ConclusionThe detection of C. botulinum in infant formula milk powder with a complex background of microorganisms should not be based on the isolation of bacteria as the gold standard but should be confirmed by a mouse toxicity test of the enrichment solution combined with a botulinum toxin antiserum protection test. Whole genome sequencing can accurately identify strains and analyze relevant genetic characteristics， providing reliable technical support for handling poisoning events. |
| first_indexed | 2024-03-07T23:35:14Z |
| format | Article |
| id | doaj.art-30d4cb5e20434fad9a47a75d7afcd132 |
| institution | Directory Open Access Journal |
| issn | 1004-8456 |
| language | zho |
| last_indexed | 2024-03-07T23:35:14Z |
| publishDate | 2023-10-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj.art-30d4cb5e20434fad9a47a75d7afcd1322024-02-20T07:51:14ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562023-10-0135101475148110.13590/j.cjfh.2023.10.0111004-8456（2023）10-1475-07Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulismLUO Haipeng0QU Hongren1DING Bo2REN Xiu3ZHAO Linna4BAI Jichao5WANG Yaping6LIN Lan7CUI Shenghui8China National Institute for Food and Drug Control, Beijing 100050, ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaYantai Food and Drug Inspection and Testing Center, Shandong Yantai 264003,ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaChina National Institute for Food and Drug Control, Beijing 100050, ChinaObjectiveThe aim of this study was to detect botulinum toxin and Clostridium botulinum （C.botulinum） in 30 batches of infant formula milk powder obtained from an enterprise and to analyze the whole genome of the strain of C. botulinum type B isolated from the samples.MethodsThe samples were subjected to C. botulinum isolation and botulinum toxin typing experiments according to the “National standard for food safety microbiological examination of C. botulinum and botulinum toxin” （GB 4789.12—2016）. The whole genomes of the isolated strains were sequenced and their genetic characteristics were analyzed.ResultsBotulinum toxin was not detected in any of the 30 batches of samples. After intraperitoneal injection of the enrichment solution into mice， four batches of milk powder samples induced symptoms of botulism， but C. botulinum was isolated from only one batch of milk powder samples. Genome sequencing revealed that the bacterium was C. botulinum group Ⅰ， type B， the toxin gene cluster was type Ha， and the toxin gene was subtype B2.ConclusionThe detection of C. botulinum in infant formula milk powder with a complex background of microorganisms should not be based on the isolation of bacteria as the gold standard but should be confirmed by a mouse toxicity test of the enrichment solution combined with a botulinum toxin antiserum protection test. Whole genome sequencing can accurately identify strains and analyze relevant genetic characteristics， providing reliable technical support for handling poisoning events.https://www.zgspws.com/zgspwszz/article/abstract/202310011?st=article_issueclostridium botulinumgenomebotulinum toxin typing |
| spellingShingle | LUO Haipeng QU Hongren DING Bo REN Xiu ZHAO Linna BAI Jichao WANG Yaping LIN Lan CUI Shenghui Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism Zhongguo shipin weisheng zazhi clostridium botulinum genome botulinum toxin typing |
| title | Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism |
| title_full | Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism |
| title_fullStr | Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism |
| title_full_unstemmed | Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism |
| title_short | Isolation and typing of Clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism |
| title_sort | isolation and typing of clostridium botulinum from milk powder of an enterprise associated with a case of infant botulism |
| topic | clostridium botulinum genome botulinum toxin typing |
| url | https://www.zgspws.com/zgspwszz/article/abstract/202310011?st=article_issue |
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