<i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System

Bovine leptospirosis is an infectious zoonotic disease causing reproductive problems and economic losses in livestock. This work reports, for the first time in Sicily (South Italy), an outbreak of <i>Leptospira interrogans</i> serogroup Pomona that occurred in cattle farms within the Neb...

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Main Authors: Antonino Aliberti, Valeria Blanda, Vincenzo Di Marco Lo Presti, Giusi Macaluso, Paola Galluzzo, Cristina Bertasio, Carmela Sciacca, Francesca Arcuri, Rosalia D’Agostino, Dorotea Ippolito, Flavia Pruiti Ciarello, Alessandra Torina, Francesca Grippi
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Veterinary Sciences
Subjects:
Online Access:https://www.mdpi.com/2306-7381/9/2/83
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author Antonino Aliberti
Valeria Blanda
Vincenzo Di Marco Lo Presti
Giusi Macaluso
Paola Galluzzo
Cristina Bertasio
Carmela Sciacca
Francesca Arcuri
Rosalia D’Agostino
Dorotea Ippolito
Flavia Pruiti Ciarello
Alessandra Torina
Francesca Grippi
author_facet Antonino Aliberti
Valeria Blanda
Vincenzo Di Marco Lo Presti
Giusi Macaluso
Paola Galluzzo
Cristina Bertasio
Carmela Sciacca
Francesca Arcuri
Rosalia D’Agostino
Dorotea Ippolito
Flavia Pruiti Ciarello
Alessandra Torina
Francesca Grippi
author_sort Antonino Aliberti
collection DOAJ
description Bovine leptospirosis is an infectious zoonotic disease causing reproductive problems and economic losses in livestock. This work reports, for the first time in Sicily (South Italy), an outbreak of <i>Leptospira interrogans</i> serogroup Pomona that occurred in cattle farms within the Nebrodi Park and was mainly characterized by full-term abortion. Blood and urine samples were collected at different time points from animals of six different farms (Farms A–F) sharing the same grazing area. Research of antibodies against pathogenic <i>Leptospira</i> species in serum samples was carried out via Micro Agglutination Test (MAT). Urine samples were subjected to pathogen isolation and molecular analyses via TaqMan Real Time-PCR. Genotyping of <i>Leptospira</i> species was obtained by Multi-locus sequence typing. MAT detected antibodies against <i>Leptospira interrogans</i> serogroup Pomona in serum samples of all the farms. Pathogenic <i>Leptospira</i> spp. DNA and culture isolation was obtained from urine samples. Genotyping confirmed the excretion of <i>L. interrogans</i> serogroup Pomona. This study describes clinical manifestations, diagnostic implications and epidemiological characteristics of an outbreak in cattle due to <i>L. interrogans</i> Pomona in a protected multi-host area, where domestic and wild animals share the same habitat, suggesting a role of wild species in transmission and persistence of Pomona serogroup among cattle.
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spelling doaj.art-30d6a139702a4c22a6ad83aa3d7d2bba2023-11-23T22:28:22ZengMDPI AGVeterinary Sciences2306-73812022-02-01928310.3390/vetsci9020083<i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical SystemAntonino Aliberti0Valeria Blanda1Vincenzo Di Marco Lo Presti2Giusi Macaluso3Paola Galluzzo4Cristina Bertasio5Carmela Sciacca6Francesca Arcuri7Rosalia D’Agostino8Dorotea Ippolito9Flavia Pruiti Ciarello10Alessandra Torina11Francesca Grippi12Istituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia, 90129 Palermo, ItalyBovine leptospirosis is an infectious zoonotic disease causing reproductive problems and economic losses in livestock. This work reports, for the first time in Sicily (South Italy), an outbreak of <i>Leptospira interrogans</i> serogroup Pomona that occurred in cattle farms within the Nebrodi Park and was mainly characterized by full-term abortion. Blood and urine samples were collected at different time points from animals of six different farms (Farms A–F) sharing the same grazing area. Research of antibodies against pathogenic <i>Leptospira</i> species in serum samples was carried out via Micro Agglutination Test (MAT). Urine samples were subjected to pathogen isolation and molecular analyses via TaqMan Real Time-PCR. Genotyping of <i>Leptospira</i> species was obtained by Multi-locus sequence typing. MAT detected antibodies against <i>Leptospira interrogans</i> serogroup Pomona in serum samples of all the farms. Pathogenic <i>Leptospira</i> spp. DNA and culture isolation was obtained from urine samples. Genotyping confirmed the excretion of <i>L. interrogans</i> serogroup Pomona. This study describes clinical manifestations, diagnostic implications and epidemiological characteristics of an outbreak in cattle due to <i>L. interrogans</i> Pomona in a protected multi-host area, where domestic and wild animals share the same habitat, suggesting a role of wild species in transmission and persistence of Pomona serogroup among cattle.https://www.mdpi.com/2306-7381/9/2/83<i>Leptospira interrogans</i> serogroup PomonacattleoutbreakSicily
spellingShingle Antonino Aliberti
Valeria Blanda
Vincenzo Di Marco Lo Presti
Giusi Macaluso
Paola Galluzzo
Cristina Bertasio
Carmela Sciacca
Francesca Arcuri
Rosalia D’Agostino
Dorotea Ippolito
Flavia Pruiti Ciarello
Alessandra Torina
Francesca Grippi
<i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System
Veterinary Sciences
<i>Leptospira interrogans</i> serogroup Pomona
cattle
outbreak
Sicily
title <i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System
title_full <i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System
title_fullStr <i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System
title_full_unstemmed <i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System
title_short <i>Leptospira interrogans</i> Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical System
title_sort i leptospira interrogans i serogroup pomona in a dairy cattle farm in a multi host zootechnical system
topic <i>Leptospira interrogans</i> serogroup Pomona
cattle
outbreak
Sicily
url https://www.mdpi.com/2306-7381/9/2/83
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