Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression
Abstract Background Extra-mammary Paget’s disease of the vulva (EMPDV) is an infrequent chronic disease that often recurs. The aim of the study was to assess the presence of neoangiogenesis and the expression of epithelial-mesenchymal transition (EMT) markers in EMPDV, and their potential correlatio...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | Diagnostic Pathology |
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| Online Access: | http://link.springer.com/article/10.1186/s13000-017-0680-x |
| _version_ | 1828791571534839808 |
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| author | Lara Alessandrini Nicolò Clemente Tiziana Perin Giorgio Giorda Vincenzo Canzonieri Francesco Sopracordevole |
| author_facet | Lara Alessandrini Nicolò Clemente Tiziana Perin Giorgio Giorda Vincenzo Canzonieri Francesco Sopracordevole |
| author_sort | Lara Alessandrini |
| collection | DOAJ |
| description | Abstract Background Extra-mammary Paget’s disease of the vulva (EMPDV) is an infrequent chronic disease that often recurs. The aim of the study was to assess the presence of neoangiogenesis and the expression of epithelial-mesenchymal transition (EMT) markers in EMPDV, and their potential correlation with stromal invasion. Methods All the women consecutively treated for EMPDV at our Institute, between January 2011 and December 2014, were studied for neoangiogenesis, analysed by microvessel density (MVD) using antibodies against CD31 and CD34. Immunohistochemical expression of E- / N-cadherin, β-catenin and SLUG was also evaluated. In each slide, three fields with the highest number of capillaries and small venules were identified at low power. In these three fields, the highest vessel density (HVD) and the average vessel density (AVD) at 200× and 400× magnification were counted. Immunohistochemical reactions for non-vascular markers were semiquantitatively scored by two pathologists, using a three-tier scale. Results Seventeen cases of EMPDV (including 10 cases of invasive disease) were included. The AVD at 200× and 400× and the HVD at 400× magnification were significantly associated with invasive EMPDV (p = 0.02, 0.03, 0.03 respectively). No significant correlation between MVD, EMT-markers expression and risk of recurrence was detected. Conclusion These results indicate that MVD, as a measure of neoangiogenesis, may be associated with histological progression of EMPDV. EMT could also be linked to an invasive potential of EMPDV but larger series are required to confirm this hypothesis. |
| first_indexed | 2024-12-12T02:53:04Z |
| format | Article |
| id | doaj.art-30d80a0994e847c7b32d16df78303855 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-12-12T02:53:04Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-30d80a0994e847c7b32d16df783038552022-12-22T00:40:49ZengBMCDiagnostic Pathology1746-15962018-01-011311810.1186/s13000-017-0680-xExtramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expressionLara Alessandrini0Nicolò Clemente1Tiziana Perin2Giorgio Giorda3Vincenzo Canzonieri4Francesco Sopracordevole5Pathology Unit - CRO - IRCCS, National Cancer InstituteGynecological Oncology Unit - CRO - IRCCS, National Cancer InstitutePathology Unit - CRO - IRCCS, National Cancer InstituteGynecological Oncology Unit - CRO - IRCCS, National Cancer InstitutePathology Unit - CRO - IRCCS, National Cancer InstituteGynecological Oncology Unit - CRO - IRCCS, National Cancer InstituteAbstract Background Extra-mammary Paget’s disease of the vulva (EMPDV) is an infrequent chronic disease that often recurs. The aim of the study was to assess the presence of neoangiogenesis and the expression of epithelial-mesenchymal transition (EMT) markers in EMPDV, and their potential correlation with stromal invasion. Methods All the women consecutively treated for EMPDV at our Institute, between January 2011 and December 2014, were studied for neoangiogenesis, analysed by microvessel density (MVD) using antibodies against CD31 and CD34. Immunohistochemical expression of E- / N-cadherin, β-catenin and SLUG was also evaluated. In each slide, three fields with the highest number of capillaries and small venules were identified at low power. In these three fields, the highest vessel density (HVD) and the average vessel density (AVD) at 200× and 400× magnification were counted. Immunohistochemical reactions for non-vascular markers were semiquantitatively scored by two pathologists, using a three-tier scale. Results Seventeen cases of EMPDV (including 10 cases of invasive disease) were included. The AVD at 200× and 400× and the HVD at 400× magnification were significantly associated with invasive EMPDV (p = 0.02, 0.03, 0.03 respectively). No significant correlation between MVD, EMT-markers expression and risk of recurrence was detected. Conclusion These results indicate that MVD, as a measure of neoangiogenesis, may be associated with histological progression of EMPDV. EMT could also be linked to an invasive potential of EMPDV but larger series are required to confirm this hypothesis.http://link.springer.com/article/10.1186/s13000-017-0680-xPaget diseaseVulvaNeoangiogenesisEMTMicrovessel densityHVD |
| spellingShingle | Lara Alessandrini Nicolò Clemente Tiziana Perin Giorgio Giorda Vincenzo Canzonieri Francesco Sopracordevole Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression Diagnostic Pathology Paget disease Vulva Neoangiogenesis EMT Microvessel density HVD |
| title | Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression |
| title_full | Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression |
| title_fullStr | Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression |
| title_full_unstemmed | Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression |
| title_short | Extramammary Paget disease of the vulva: immunohistochemical analysis of neoangiogenesis and epithelial-mesenchymal transition markers expression |
| title_sort | extramammary paget disease of the vulva immunohistochemical analysis of neoangiogenesis and epithelial mesenchymal transition markers expression |
| topic | Paget disease Vulva Neoangiogenesis EMT Microvessel density HVD |
| url | http://link.springer.com/article/10.1186/s13000-017-0680-x |
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