Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis
Introduction: Hemicellulose is an important component in lignocellulose materials, which is second only to cellulose, accounting for 15%–35% of the dry weight of plants. In the current situation of energy shortage, making full use of lignocellulose materials to produce fuel ethanol has become an imp...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1095323/full |
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| author | Ning Zhang Ning Zhang Yue Jiang Yue Jiang Yun-Juan Sun Yun-Juan Sun Jian-Chun Jiang Jian-Chun Jiang Ya-Juan Tong Ya-Juan Tong |
| author_facet | Ning Zhang Ning Zhang Yue Jiang Yue Jiang Yun-Juan Sun Yun-Juan Sun Jian-Chun Jiang Jian-Chun Jiang Ya-Juan Tong Ya-Juan Tong |
| author_sort | Ning Zhang |
| collection | DOAJ |
| description | Introduction: Hemicellulose is an important component in lignocellulose materials, which is second only to cellulose, accounting for 15%–35% of the dry weight of plants. In the current situation of energy shortage, making full use of lignocellulose materials to produce fuel ethanol has become an important way to solve the energy problem. Xylanase plays a crucial role in the utilization of hemicellulose. It is a necessary means to reduce the cost of hemicellulose utilization by improving the activity of xylanase. Moreover, most naturally xylanases are mesophilic enzymes, which limits their industrial application.Methods:In this study, Myceliophthora thermophila was used to produce xylanases and a thermostable mutant M 2103 was obtained by atmospheric room temperature plasma (ARTP) mutagenesis. The research work started with exploring the effects of ARTP mutagenesis on the antioxidase system [superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), and antioxidant capacity (AOC)] of M. thermophile, and found that superoxide dismutase activity increased by 221.13%, and polyphenol oxidase activity increased by 486.04% as compared with the original strain when the implantation time was 300 s. So as to determine the conditions for subsequent mutagenesis.Results and Discussion:For the mutant M 2103, the reaction temperature for xylanase production remained stable in the range of 70°C–85°C. Its optimum temperature was 75°C, which was 15°C higher than that of the original strain. And its xylanase activity increased by 21.71% as compared with the original strain. M 2103 displayed a significantly higher relative xylanase activity than the original strain in the acidic (pH 4.0–7.0) range, and the xylanase activity was relatively stable in the pH range of 6.0–8.5. These results provide an alternative biocatalyst for the production of xylooligosaccharide, and a potential usage of ARTP in the mutagenesis of thermostable mutant. |
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| language | English |
| last_indexed | 2024-04-11T00:49:50Z |
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| spelling | doaj.art-30dc6fa4e0c04e458c9c22a3f96cef512023-01-05T09:13:06ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.10953231095323Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesisNing Zhang0Ning Zhang1Yue Jiang2Yue Jiang3Yun-Juan Sun4Yun-Juan Sun5Jian-Chun Jiang6Jian-Chun Jiang7Ya-Juan Tong8Ya-Juan Tong9Key Lab of Biomass Energy and Material, Key Lab of Chemical Engineering of Forest Products, National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resource, National Forestry and Grassland Administration, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu, ChinaCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaKey Lab of Biomass Energy and Material, Key Lab of Chemical Engineering of Forest Products, National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resource, National Forestry and Grassland Administration, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu, ChinaCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaKey Lab of Biomass Energy and Material, Key Lab of Chemical Engineering of Forest Products, National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resource, National Forestry and Grassland Administration, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu, ChinaCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaKey Lab of Biomass Energy and Material, Key Lab of Chemical Engineering of Forest Products, National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resource, National Forestry and Grassland Administration, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu, ChinaCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaKey Lab of Biomass Energy and Material, Key Lab of Chemical Engineering of Forest Products, National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resource, National Forestry and Grassland Administration, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry (CAF), Nanjing, Jiangsu, ChinaCo-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, ChinaIntroduction: Hemicellulose is an important component in lignocellulose materials, which is second only to cellulose, accounting for 15%–35% of the dry weight of plants. In the current situation of energy shortage, making full use of lignocellulose materials to produce fuel ethanol has become an important way to solve the energy problem. Xylanase plays a crucial role in the utilization of hemicellulose. It is a necessary means to reduce the cost of hemicellulose utilization by improving the activity of xylanase. Moreover, most naturally xylanases are mesophilic enzymes, which limits their industrial application.Methods:In this study, Myceliophthora thermophila was used to produce xylanases and a thermostable mutant M 2103 was obtained by atmospheric room temperature plasma (ARTP) mutagenesis. The research work started with exploring the effects of ARTP mutagenesis on the antioxidase system [superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), polyphenol oxidase (PPO), and antioxidant capacity (AOC)] of M. thermophile, and found that superoxide dismutase activity increased by 221.13%, and polyphenol oxidase activity increased by 486.04% as compared with the original strain when the implantation time was 300 s. So as to determine the conditions for subsequent mutagenesis.Results and Discussion:For the mutant M 2103, the reaction temperature for xylanase production remained stable in the range of 70°C–85°C. Its optimum temperature was 75°C, which was 15°C higher than that of the original strain. And its xylanase activity increased by 21.71% as compared with the original strain. M 2103 displayed a significantly higher relative xylanase activity than the original strain in the acidic (pH 4.0–7.0) range, and the xylanase activity was relatively stable in the pH range of 6.0–8.5. These results provide an alternative biocatalyst for the production of xylooligosaccharide, and a potential usage of ARTP in the mutagenesis of thermostable mutant.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1095323/fullxylanaseMyceliophthora thermophilathermotolerant mutantantioxidaseARTP |
| spellingShingle | Ning Zhang Ning Zhang Yue Jiang Yue Jiang Yun-Juan Sun Yun-Juan Sun Jian-Chun Jiang Jian-Chun Jiang Ya-Juan Tong Ya-Juan Tong Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis Frontiers in Bioengineering and Biotechnology xylanase Myceliophthora thermophila thermotolerant mutant antioxidase ARTP |
| title | Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis |
| title_full | Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis |
| title_fullStr | Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis |
| title_full_unstemmed | Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis |
| title_short | Breeding of a thermostable xylanase-producing strain of Myceliophthora thermophila by atmospheric room temperature plasma (ARTP) mutagenesis |
| title_sort | breeding of a thermostable xylanase producing strain of myceliophthora thermophila by atmospheric room temperature plasma artp mutagenesis |
| topic | xylanase Myceliophthora thermophila thermotolerant mutant antioxidase ARTP |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1095323/full |
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