Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma
<p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinases (MMPs) are one of the major classes of proteolytic enzymes involved in tumor invasion and metastasis and are inhibited by naturally occurring tissue inhibitors of metalloproteinases (TIMPs). {AU Query: Pleas...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2013-01-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | http://www.wjso.com/content/11/1/1 |
| _version_ | 1818389358798962688 |
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| author | Qiao Zhen-kui Li Yan-long Lu Hong-tao Wang Ke-liang Xu Wan-hai |
| author_facet | Qiao Zhen-kui Li Yan-long Lu Hong-tao Wang Ke-liang Xu Wan-hai |
| author_sort | Qiao Zhen-kui |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinases (MMPs) are one of the major classes of proteolytic enzymes involved in tumor invasion and metastasis and are inhibited by naturally occurring tissue inhibitors of metalloproteinases (TIMPs). {AU Query: Please verify that corrections made to previous sentence did not alter intended meaning}. In this study, we examined the expression of MMP-2, MMP-9, membrane-type 1 (MT1)-MMP, TIMP-1, and TIMP-2 in renal tissue samples of renal cell cancer and examined the correlation between their expression and clinicopathological parameters.</p> <p>Methods</p> <p>Renal tissue samples from 76 patients with renal cell carcinoma were available for this study. To determine the expression of MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out on tumor and normal tissues.</p> <p>Results</p> <p>Mean MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA expression in the renal cell carcinomas was significantly higher than in the normal renal tissue (<it>P</it> <0.05). The RT-PCR data of MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 did not show any significant correlation with tumor type or pathologic grade of renal cell carcinoma. MMP-2, MMP-9 and MT1-MMP mRNA expression increased significantly with the TNM stage of the tumor.</p> <p>Conclusions</p> <p>Mean MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA expression in the renal cell carcinomas was significantly higher than in the normal renal tissue.</p> |
| first_indexed | 2024-12-14T04:40:28Z |
| format | Article |
| id | doaj.art-30e09ad9d2ea4800930efab3b689e5e5 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-14T04:40:28Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-30e09ad9d2ea4800930efab3b689e5e52022-12-21T23:16:48ZengBMCWorld Journal of Surgical Oncology1477-78192013-01-01111110.1186/1477-7819-11-1Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinomaQiao Zhen-kuiLi Yan-longLu Hong-taoWang Ke-liangXu Wan-hai<p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinases (MMPs) are one of the major classes of proteolytic enzymes involved in tumor invasion and metastasis and are inhibited by naturally occurring tissue inhibitors of metalloproteinases (TIMPs). {AU Query: Please verify that corrections made to previous sentence did not alter intended meaning}. In this study, we examined the expression of MMP-2, MMP-9, membrane-type 1 (MT1)-MMP, TIMP-1, and TIMP-2 in renal tissue samples of renal cell cancer and examined the correlation between their expression and clinicopathological parameters.</p> <p>Methods</p> <p>Renal tissue samples from 76 patients with renal cell carcinoma were available for this study. To determine the expression of MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out on tumor and normal tissues.</p> <p>Results</p> <p>Mean MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA expression in the renal cell carcinomas was significantly higher than in the normal renal tissue (<it>P</it> <0.05). The RT-PCR data of MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 did not show any significant correlation with tumor type or pathologic grade of renal cell carcinoma. MMP-2, MMP-9 and MT1-MMP mRNA expression increased significantly with the TNM stage of the tumor.</p> <p>Conclusions</p> <p>Mean MMP-2, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA expression in the renal cell carcinomas was significantly higher than in the normal renal tissue.</p>http://www.wjso.com/content/11/1/1Matrix metalloproteinaseTissue inhibitor of metalloproteinaseSemi-quantitative RT-PCRRenal cell carcinoma |
| spellingShingle | Qiao Zhen-kui Li Yan-long Lu Hong-tao Wang Ke-liang Xu Wan-hai Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma World Journal of Surgical Oncology Matrix metalloproteinase Tissue inhibitor of metalloproteinase Semi-quantitative RT-PCR Renal cell carcinoma |
| title | Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma |
| title_full | Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma |
| title_fullStr | Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma |
| title_full_unstemmed | Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma |
| title_short | Expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma |
| title_sort | expression of tissue levels of matrix metalloproteinases and tissue inhibitors of metalloproteinases in renal cell carcinoma |
| topic | Matrix metalloproteinase Tissue inhibitor of metalloproteinase Semi-quantitative RT-PCR Renal cell carcinoma |
| url | http://www.wjso.com/content/11/1/1 |
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