Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior
Summary: The gut microbiota can affect how animals respond to ingested toxins, such as ethanol, which is prevalent in the diets of diverse animals and often leads to negative health outcomes in humans. Ethanol is a complex dietary factor because it acts as a toxin, behavioral manipulator, and nutrit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422200270X |
| _version_ | 1828858713059885056 |
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| author | James Angus Chandler Lina Victoria Innocent Daniel Jonathan Martinez Isaac Li Huang Jane Lani Yang Michael Bruce Eisen William Basil Ludington |
| author_facet | James Angus Chandler Lina Victoria Innocent Daniel Jonathan Martinez Isaac Li Huang Jane Lani Yang Michael Bruce Eisen William Basil Ludington |
| author_sort | James Angus Chandler |
| collection | DOAJ |
| description | Summary: The gut microbiota can affect how animals respond to ingested toxins, such as ethanol, which is prevalent in the diets of diverse animals and often leads to negative health outcomes in humans. Ethanol is a complex dietary factor because it acts as a toxin, behavioral manipulator, and nutritional source, with both direct effects on the host as well as indirect ones through the microbiome. Here, we developed a model for chronic, non-intoxicating ethanol ingestion in the adult fruit fly, Drosophila melanogaster, and paired this with the tractability of the fly gut microbiota, which can be experimentally removed. We linked numerous physiological, behavioral, and transcriptional variables to fly fitness, including a combination of intestinal barrier integrity, stored triglyceride levels, feeding behavior, and the immunodeficiency pathway. Our results reveal a complex tradeoff between lifespan and fecundity that is microbiome-dependent and modulated by dietary ethanol and feeding behavior. |
| first_indexed | 2024-12-13T02:03:27Z |
| format | Article |
| id | doaj.art-30e2b09d951b4ee8bc2257f59908faef |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-13T02:03:27Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-30e2b09d951b4ee8bc2257f59908faef2022-12-22T00:03:13ZengElsevieriScience2589-00422022-04-01254104000Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behaviorJames Angus Chandler0Lina Victoria Innocent1Daniel Jonathan Martinez2Isaac Li Huang3Jane Lani Yang4Michael Bruce Eisen5William Basil Ludington6Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Corresponding authorDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USADepartment of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USADepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USADepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USADepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Department of Integrative Biology, University of California, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USADepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, USA; Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA; Corresponding authorSummary: The gut microbiota can affect how animals respond to ingested toxins, such as ethanol, which is prevalent in the diets of diverse animals and often leads to negative health outcomes in humans. Ethanol is a complex dietary factor because it acts as a toxin, behavioral manipulator, and nutritional source, with both direct effects on the host as well as indirect ones through the microbiome. Here, we developed a model for chronic, non-intoxicating ethanol ingestion in the adult fruit fly, Drosophila melanogaster, and paired this with the tractability of the fly gut microbiota, which can be experimentally removed. We linked numerous physiological, behavioral, and transcriptional variables to fly fitness, including a combination of intestinal barrier integrity, stored triglyceride levels, feeding behavior, and the immunodeficiency pathway. Our results reveal a complex tradeoff between lifespan and fecundity that is microbiome-dependent and modulated by dietary ethanol and feeding behavior.http://www.sciencedirect.com/science/article/pii/S258900422200270XBiological sciencesGeneticsMicrobiologyMicrobiomeMolecular biologyPhysiology |
| spellingShingle | James Angus Chandler Lina Victoria Innocent Daniel Jonathan Martinez Isaac Li Huang Jane Lani Yang Michael Bruce Eisen William Basil Ludington Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior iScience Biological sciences Genetics Microbiology Microbiome Molecular biology Physiology |
| title | Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior |
| title_full | Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior |
| title_fullStr | Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior |
| title_full_unstemmed | Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior |
| title_short | Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior |
| title_sort | microbiome by ethanol interactions impact drosophila melanogaster fitness physiology and behavior |
| topic | Biological sciences Genetics Microbiology Microbiome Molecular biology Physiology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422200270X |
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