HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway
Abstract Background Heat shock protein B7 (HSPB7), which belongs to small heat shock protein family, has been reported to be involved in diverse biological processes and diseases. However, whether HSPB7 regulates osteogenic differentiation of human adipose derived stem cells (hASCs) remains unexplor...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | http://link.springer.com/article/10.1186/s13287-020-01965-4 |
| _version_ | 1818245100773310464 |
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| author | Chanyuan Jin Ting Shuai Zhihui Tang |
| author_facet | Chanyuan Jin Ting Shuai Zhihui Tang |
| author_sort | Chanyuan Jin |
| collection | DOAJ |
| description | Abstract Background Heat shock protein B7 (HSPB7), which belongs to small heat shock protein family, has been reported to be involved in diverse biological processes and diseases. However, whether HSPB7 regulates osteogenic differentiation of human adipose derived stem cells (hASCs) remains unexplored. Methods The expression level of HSPB7 during the osteogenesis of hASCs was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. Lentivirus transfection was used to knock down or overexpress HSPB7, which enabled us to investigate the effect of HSPB7 on osteogenic differentiation of hASCs. U0126 and extracellular signal-regulated protein kinase 1/2 (ERK1/2) siRNA were used to identify the mechanism of the HSPB7/ERK1/2 axis in regulating osteogenic differentiation of hASCs. Moreover, ectopic bone formation in nude mice and osteoporosis mice model was used to investigate the effect of HSPB7 on osteogenesis in vivo. Results In this study, we found the expression of HSPB7 was significantly downregulated during the osteogenic differentiation of hASCs. HSPB7 knockdown remarkably promoted osteogenic differentiation of hASCs, while HSPB7 overexpression suppressed osteogenic differentiation of hASCs both in vitro and in vivo. Moreover, we discovered that the enhancing effect of HSPB7 knockdown on osteogenic differentiation was related to the activation of extracellular signal-regulated protein kinase (ERK) signaling pathway. Inhibition of ERK signaling pathway with U0126 or silencing ERK1/2 effectively blocked the stimulation of osteogenic differentiation induced by HSPB7 knockdown. Additionally, we found that HSPB7 expression was markedly increased in mouse bone marrow mesenchymal stem cells (mBMSCs) from the osteoporotic mice which suggested that HSPB7 might be utilized as a potential target in the development of effective therapeutic strategies to treat osteoporosis and other bone diseases. Conclusion Taken together, these findings uncover a previously unrecognized function of HSPB7 in regulating osteogenic differentiation of hASCs, partly via the ERK signaling pathway. |
| first_indexed | 2024-12-12T14:27:33Z |
| format | Article |
| id | doaj.art-30e59bc1c7154c8d87d9999df66be524 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-12T14:27:33Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-30e59bc1c7154c8d87d9999df66be5242022-12-22T00:21:38ZengBMCStem Cell Research & Therapy1757-65122020-10-0111111310.1186/s13287-020-01965-4HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathwayChanyuan Jin0Ting Shuai1Zhihui Tang2Second Clinical Division, Peking University School and Hospital of StomatologySecond Clinical Division, Peking University School and Hospital of StomatologySecond Clinical Division, Peking University School and Hospital of StomatologyAbstract Background Heat shock protein B7 (HSPB7), which belongs to small heat shock protein family, has been reported to be involved in diverse biological processes and diseases. However, whether HSPB7 regulates osteogenic differentiation of human adipose derived stem cells (hASCs) remains unexplored. Methods The expression level of HSPB7 during the osteogenesis of hASCs was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. Lentivirus transfection was used to knock down or overexpress HSPB7, which enabled us to investigate the effect of HSPB7 on osteogenic differentiation of hASCs. U0126 and extracellular signal-regulated protein kinase 1/2 (ERK1/2) siRNA were used to identify the mechanism of the HSPB7/ERK1/2 axis in regulating osteogenic differentiation of hASCs. Moreover, ectopic bone formation in nude mice and osteoporosis mice model was used to investigate the effect of HSPB7 on osteogenesis in vivo. Results In this study, we found the expression of HSPB7 was significantly downregulated during the osteogenic differentiation of hASCs. HSPB7 knockdown remarkably promoted osteogenic differentiation of hASCs, while HSPB7 overexpression suppressed osteogenic differentiation of hASCs both in vitro and in vivo. Moreover, we discovered that the enhancing effect of HSPB7 knockdown on osteogenic differentiation was related to the activation of extracellular signal-regulated protein kinase (ERK) signaling pathway. Inhibition of ERK signaling pathway with U0126 or silencing ERK1/2 effectively blocked the stimulation of osteogenic differentiation induced by HSPB7 knockdown. Additionally, we found that HSPB7 expression was markedly increased in mouse bone marrow mesenchymal stem cells (mBMSCs) from the osteoporotic mice which suggested that HSPB7 might be utilized as a potential target in the development of effective therapeutic strategies to treat osteoporosis and other bone diseases. Conclusion Taken together, these findings uncover a previously unrecognized function of HSPB7 in regulating osteogenic differentiation of hASCs, partly via the ERK signaling pathway.http://link.springer.com/article/10.1186/s13287-020-01965-4OsteogenesisBone formationHeat shock proteincvHSP |
| spellingShingle | Chanyuan Jin Ting Shuai Zhihui Tang HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway Stem Cell Research & Therapy Osteogenesis Bone formation Heat shock protein cvHSP |
| title | HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway |
| title_full | HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway |
| title_fullStr | HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway |
| title_full_unstemmed | HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway |
| title_short | HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway |
| title_sort | hspb7 regulates osteogenic differentiation of human adipose derived stem cells via erk signaling pathway |
| topic | Osteogenesis Bone formation Heat shock protein cvHSP |
| url | http://link.springer.com/article/10.1186/s13287-020-01965-4 |
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