Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice
Background: The novel coronavirus disease 2019 (COVID-19) has raised many concerns among populations worldwide due to its high mortality and morbidity with the lack of treatment options. Many attempts to find preventive and curable approaches have been proposed to prevent infections. The consumption...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266714252300115X |
| _version_ | 1827581912642748416 |
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| author | Minh-Nhut Pham Phu-Tuong Nguyen-Dung Thi-Kim-Nga Nguyen Viet-Hung Tran Nguyen-Truong-Thang Phan Thi-Hong-Tuoi Do Thuy-Linh Hoang Thi-To-Uyen Nguyen Phu-Tho Nguyen Huu-Thanh Nguyen |
| author_facet | Minh-Nhut Pham Phu-Tuong Nguyen-Dung Thi-Kim-Nga Nguyen Viet-Hung Tran Nguyen-Truong-Thang Phan Thi-Hong-Tuoi Do Thuy-Linh Hoang Thi-To-Uyen Nguyen Phu-Tho Nguyen Huu-Thanh Nguyen |
| author_sort | Minh-Nhut Pham |
| collection | DOAJ |
| description | Background: The novel coronavirus disease 2019 (COVID-19) has raised many concerns among populations worldwide due to its high mortality and morbidity with the lack of treatment options. Many attempts to find preventive and curable approaches have been proposed to prevent infections. The consumption of herbal products or plant extracts was recommended to modulate the body's immunity and reduce the risk of diseases. Methods: In the current study, we evaluated the anti-viral effects of a mixed herbal formula, named Ssanti-Covir, which was obtained from a combination of 8 herbal species extensively employed in traditional medicine in Vietnam. The objective was to investigate the potential efficacy of Ssanti-Covir against COVID-19 infections in vero E6 cells, as well as its immunostimulatory effects on immunosuppressive mice induced by cyclophosphamide (CP). Results: The results of the study revealed that Ssanti-Covir contained significant amounts of andrographolide, silybin A, and silybin B, with concentrations of 137.08 mg/g, 72.01 mg/g, and 81.62 mg/g, respectively. Pre-incubation of Ssanti-Covir at various dilution concentrations effectively prevented cells from being infected by COVID-19, with a viral titer of 100 TCID50/0.1 ml. Oral administration of Ssanti-Covir at doses of 384 mg/kg and 576 mg/kg body weight resulted in a significant increase in the relative weight of the spleen and thymus in mice induced with CP, with p-values of <0.05 and <0.01, respectively, as well as elevated levels of peripheral white blood cells, lymphocytes, neutrophils, and monocytes. Furthermore, all three dosage regimens (192 mg/kg, 384 mg/kg, and 576 mg/kg) also elevated the levels of IL-4, IL-6, TNF-α, IFN-γ cytokines, and IgG in the mice. Conclusion: The research findings suggest that the use of Ssanti-Covir exhibits significant efficacy in preventing COVID-19 infections, while also demonstrating immunostimulatory effects against the CP-induced immunosuppressive model. Consequently, Ssanti-Covir could be employed as a valuable preventive measure or adjuvant treatment for SARS-CoV-2 infections. |
| first_indexed | 2024-03-08T22:43:04Z |
| format | Article |
| id | doaj.art-30e5ad3241334eecb19d620107f545f0 |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-03-08T22:43:04Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-30e5ad3241334eecb19d620107f545f02023-12-17T06:43:01ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252023-12-019100329Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated miceMinh-Nhut Pham0Phu-Tuong Nguyen-Dung1Thi-Kim-Nga Nguyen2Viet-Hung Tran3Nguyen-Truong-Thang Phan4Thi-Hong-Tuoi Do5Thuy-Linh Hoang6Thi-To-Uyen Nguyen7Phu-Tho Nguyen8Huu-Thanh Nguyen9Institute for Coastal Herbal Medicine, SSAVIGROUP Joint Stock Company, Ben Tre, Viet Nam; Hutech Institute of Applied Science, HUTECH University, Ho Chi Minh City, Viet NamInstitute for Coastal Herbal Medicine, SSAVIGROUP Joint Stock Company, Ben Tre, Viet NamInstitute for Coastal Herbal Medicine, SSAVIGROUP Joint Stock Company, Ben Tre, Viet NamInstitute of Drug Quality Control-Ho Chi Minh City (IDQC HCMC), Ho Chi Minh City 700000, Viet NamInstitute of Drug Quality Control-Ho Chi Minh City (IDQC HCMC), Ho Chi Minh City 700000, Viet NamFaculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City (UMP HCMC), Ho Chi Minh City 700000, Viet NamPasteur Institute in Ho Chi Minh City (PI HCMC), Ho Chi Minh City 700000, Viet NamDong Thap Medical College, Dong Thap, Viet NamAn Giang University, An Giang, Viet Nam; Vietnam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet Nam; Corresponding author at: An Giang University, An Giang, Viet Nam.An Giang University, An Giang, Viet Nam; Vietnam National University Ho Chi Minh City (VNU-HCM), Ho Chi Minh City, Viet NamBackground: The novel coronavirus disease 2019 (COVID-19) has raised many concerns among populations worldwide due to its high mortality and morbidity with the lack of treatment options. Many attempts to find preventive and curable approaches have been proposed to prevent infections. The consumption of herbal products or plant extracts was recommended to modulate the body's immunity and reduce the risk of diseases. Methods: In the current study, we evaluated the anti-viral effects of a mixed herbal formula, named Ssanti-Covir, which was obtained from a combination of 8 herbal species extensively employed in traditional medicine in Vietnam. The objective was to investigate the potential efficacy of Ssanti-Covir against COVID-19 infections in vero E6 cells, as well as its immunostimulatory effects on immunosuppressive mice induced by cyclophosphamide (CP). Results: The results of the study revealed that Ssanti-Covir contained significant amounts of andrographolide, silybin A, and silybin B, with concentrations of 137.08 mg/g, 72.01 mg/g, and 81.62 mg/g, respectively. Pre-incubation of Ssanti-Covir at various dilution concentrations effectively prevented cells from being infected by COVID-19, with a viral titer of 100 TCID50/0.1 ml. Oral administration of Ssanti-Covir at doses of 384 mg/kg and 576 mg/kg body weight resulted in a significant increase in the relative weight of the spleen and thymus in mice induced with CP, with p-values of <0.05 and <0.01, respectively, as well as elevated levels of peripheral white blood cells, lymphocytes, neutrophils, and monocytes. Furthermore, all three dosage regimens (192 mg/kg, 384 mg/kg, and 576 mg/kg) also elevated the levels of IL-4, IL-6, TNF-α, IFN-γ cytokines, and IgG in the mice. Conclusion: The research findings suggest that the use of Ssanti-Covir exhibits significant efficacy in preventing COVID-19 infections, while also demonstrating immunostimulatory effects against the CP-induced immunosuppressive model. Consequently, Ssanti-Covir could be employed as a valuable preventive measure or adjuvant treatment for SARS-CoV-2 infections.http://www.sciencedirect.com/science/article/pii/S266714252300115XAndrographolideImmunostimulatory effectPlant extractSARS-CoV-2Silybin |
| spellingShingle | Minh-Nhut Pham Phu-Tuong Nguyen-Dung Thi-Kim-Nga Nguyen Viet-Hung Tran Nguyen-Truong-Thang Phan Thi-Hong-Tuoi Do Thuy-Linh Hoang Thi-To-Uyen Nguyen Phu-Tho Nguyen Huu-Thanh Nguyen Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice Pharmacological Research - Modern Chinese Medicine Andrographolide Immunostimulatory effect Plant extract SARS-CoV-2 Silybin |
| title | Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice |
| title_full | Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice |
| title_fullStr | Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice |
| title_full_unstemmed | Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice |
| title_short | Antiviral and immunostimulatory effects of Ssanti-Covir, a mixed herbal formulation, in cyclophosphamide-treated mice |
| title_sort | antiviral and immunostimulatory effects of ssanti covir a mixed herbal formulation in cyclophosphamide treated mice |
| topic | Andrographolide Immunostimulatory effect Plant extract SARS-CoV-2 Silybin |
| url | http://www.sciencedirect.com/science/article/pii/S266714252300115X |
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