Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers
Medicago sativa (Alfalfa) is valued for its high nutrient content as livestock feed for the production of dairy and meat products globally. The genetic improvement of alfalfa is limited because it is an allogamous autotetraploid. In this study, Inter-simple sequence repeat (ISSR) and start codon tar...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2023.2194187 |
| _version_ | 1797215141106810880 |
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| author | Othman R. Alzahrani M. Ali Alshehri A. Alasmari S. D. Ibrahim Atif A. Oyouni Zahid H. Siddiqui |
| author_facet | Othman R. Alzahrani M. Ali Alshehri A. Alasmari S. D. Ibrahim Atif A. Oyouni Zahid H. Siddiqui |
| author_sort | Othman R. Alzahrani |
| collection | DOAJ |
| description | Medicago sativa (Alfalfa) is valued for its high nutrient content as livestock feed for the production of dairy and meat products globally. The genetic improvement of alfalfa is limited because it is an allogamous autotetraploid. In this study, Inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) markers were used to assess the extent of genetic divergence between sixteen cultivars: twelve from Saudi Arabia and four from Egypt. The genetic diversity was assessed using eleven ISSR and eight SCoT primers. The ISSR primers produce 163 amplified bands with an average of 14.82 bands per primer and 60% polymorphism. The SCoT primers reveal 150 bands, with an average of 18.75 bands per primer and 77% polymorphism. The dendrogram clustered the Saudi and Egyptian cultivars into two distinct populations. The information revealed here will help in the breeding program of alfalfa, which will produce more drought tolerant and high-yielding varieties. |
| first_indexed | 2024-03-10T08:13:26Z |
| format | Article |
| id | doaj.art-30e80ce1e3d047daa8e2c1849d1def29 |
| institution | Directory Open Access Journal |
| issn | 1658-3655 |
| language | English |
| last_indexed | 2024-04-24T11:25:21Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj.art-30e80ce1e3d047daa8e2c1849d1def292024-04-10T20:17:48ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552023-12-0117110.1080/16583655.2023.2194187Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markersOthman R. Alzahrani0M. Ali Alshehri1A. Alasmari2S. D. Ibrahim3Atif A. Oyouni4Zahid H. Siddiqui5Department of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaAgricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, EgyptDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaDepartment of Biology, Faculty of Science, University of Tabuk, Tabuk, Saudi ArabiaMedicago sativa (Alfalfa) is valued for its high nutrient content as livestock feed for the production of dairy and meat products globally. The genetic improvement of alfalfa is limited because it is an allogamous autotetraploid. In this study, Inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) markers were used to assess the extent of genetic divergence between sixteen cultivars: twelve from Saudi Arabia and four from Egypt. The genetic diversity was assessed using eleven ISSR and eight SCoT primers. The ISSR primers produce 163 amplified bands with an average of 14.82 bands per primer and 60% polymorphism. The SCoT primers reveal 150 bands, with an average of 18.75 bands per primer and 77% polymorphism. The dendrogram clustered the Saudi and Egyptian cultivars into two distinct populations. The information revealed here will help in the breeding program of alfalfa, which will produce more drought tolerant and high-yielding varieties.https://www.tandfonline.com/doi/10.1080/16583655.2023.2194187Genetic diversityalfalfaISSRSCoT Markersgenetic improvement |
| spellingShingle | Othman R. Alzahrani M. Ali Alshehri A. Alasmari S. D. Ibrahim Atif A. Oyouni Zahid H. Siddiqui Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers Journal of Taibah University for Science Genetic diversity alfalfa ISSR SCoT Markers genetic improvement |
| title | Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers |
| title_full | Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers |
| title_fullStr | Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers |
| title_full_unstemmed | Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers |
| title_short | Evaluation of genetic diversity among Saudi Arabian and Egyptian cultivars of alfalfa (Medicago sativa L.) using ISSR and SCoT markers |
| title_sort | evaluation of genetic diversity among saudi arabian and egyptian cultivars of alfalfa medicago sativa l using issr and scot markers |
| topic | Genetic diversity alfalfa ISSR SCoT Markers genetic improvement |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2023.2194187 |
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