A Portable Device for Simple Exosome Separation from Biological Samples
Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/10/1182 |
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| author | Wenwen Chen Yingying Xie Yuang Chang Yuhai Xu Mengqian Zhao Pengwei Deng Jianhua Qin Hongjing Li |
| author_facet | Wenwen Chen Yingying Xie Yuang Chang Yuhai Xu Mengqian Zhao Pengwei Deng Jianhua Qin Hongjing Li |
| author_sort | Wenwen Chen |
| collection | DOAJ |
| description | Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis. |
| first_indexed | 2024-03-10T06:22:48Z |
| format | Article |
| id | doaj.art-30e9b735a2b040718b9a4f5014e56994 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T06:22:48Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-30e9b735a2b040718b9a4f5014e569942023-11-22T19:11:06ZengMDPI AGMicromachines2072-666X2021-09-011210118210.3390/mi12101182A Portable Device for Simple Exosome Separation from Biological SamplesWenwen Chen0Yingying Xie1Yuang Chang2Yuhai Xu3Mengqian Zhao4Pengwei Deng5Jianhua Qin6Hongjing Li7Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaFirst Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaFirst Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaDalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, ChinaFirst Affiliated Hospital of Dalian Medical University, Dalian 116011, ChinaExosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis.https://www.mdpi.com/2072-666X/12/10/1182exosomeseparationchitosan scaffold |
| spellingShingle | Wenwen Chen Yingying Xie Yuang Chang Yuhai Xu Mengqian Zhao Pengwei Deng Jianhua Qin Hongjing Li A Portable Device for Simple Exosome Separation from Biological Samples Micromachines exosome separation chitosan scaffold |
| title | A Portable Device for Simple Exosome Separation from Biological Samples |
| title_full | A Portable Device for Simple Exosome Separation from Biological Samples |
| title_fullStr | A Portable Device for Simple Exosome Separation from Biological Samples |
| title_full_unstemmed | A Portable Device for Simple Exosome Separation from Biological Samples |
| title_short | A Portable Device for Simple Exosome Separation from Biological Samples |
| title_sort | portable device for simple exosome separation from biological samples |
| topic | exosome separation chitosan scaffold |
| url | https://www.mdpi.com/2072-666X/12/10/1182 |
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