Silicon microgrooves for contact guidance of human aortic endothelial cells
Background: Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium f...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Beilstein-Institut
2017-03-01
|
| Series: | Beilstein Journal of Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjnano.8.72 |
| _version_ | 1818942916650860544 |
|---|---|
| author | Sara Fernández-Castillejo Pilar Formentín Úrsula Catalán Josep Pallarès Lluís F. Marsal Rosa Solà |
| author_facet | Sara Fernández-Castillejo Pilar Formentín Úrsula Catalán Josep Pallarès Lluís F. Marsal Rosa Solà |
| author_sort | Sara Fernández-Castillejo |
| collection | DOAJ |
| description | Background: Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to use them as substrates to study cell growth and behaviour.Results: The influence of groove-shaped substrates on cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell adhesion, morphology (alignment, circularity and filopodia presence) and proliferation. Moreover, these structures exerted no cytotoxic effect.Conclusion: The results suggest that topographical patterning influences cell response. Silicon groove substrates can be used in developing medical devices with microscale features to mimic the endothelium in lineal vessels. |
| first_indexed | 2024-12-20T07:19:02Z |
| format | Article |
| id | doaj.art-30eac1adc98846aaa6db47ed713aa908 |
| institution | Directory Open Access Journal |
| issn | 2190-4286 |
| language | English |
| last_indexed | 2024-12-20T07:19:02Z |
| publishDate | 2017-03-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Nanotechnology |
| spelling | doaj.art-30eac1adc98846aaa6db47ed713aa9082022-12-21T19:48:43ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862017-03-018167568110.3762/bjnano.8.722190-4286-8-72Silicon microgrooves for contact guidance of human aortic endothelial cellsSara Fernández-Castillejo0Pilar Formentín1Úrsula Catalán2Josep Pallarès3Lluís F. Marsal4Rosa Solà5Unit of Lipids and Atherosclerosis Research, Department of Medicine and Surgery, Universitat Rovira I Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, SpainNano-electronic and Photonic Systems, Departament d’Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira I Virgili, Països Catalans 26, 43007 Tarragona, SpainUnit of Lipids and Atherosclerosis Research, Department of Medicine and Surgery, Universitat Rovira I Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, SpainNano-electronic and Photonic Systems, Departament d’Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira I Virgili, Països Catalans 26, 43007 Tarragona, SpainNano-electronic and Photonic Systems, Departament d’Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira I Virgili, Països Catalans 26, 43007 Tarragona, SpainUnit of Lipids and Atherosclerosis Research, Department of Medicine and Surgery, Universitat Rovira I Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, SpainBackground: Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to use them as substrates to study cell growth and behaviour.Results: The influence of groove-shaped substrates on cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell adhesion, morphology (alignment, circularity and filopodia presence) and proliferation. Moreover, these structures exerted no cytotoxic effect.Conclusion: The results suggest that topographical patterning influences cell response. Silicon groove substrates can be used in developing medical devices with microscale features to mimic the endothelium in lineal vessels.https://doi.org/10.3762/bjnano.8.72cell morphologycontact guidancemicrogroovessiliconhuman aortic endothelial cells (HAECs) |
| spellingShingle | Sara Fernández-Castillejo Pilar Formentín Úrsula Catalán Josep Pallarès Lluís F. Marsal Rosa Solà Silicon microgrooves for contact guidance of human aortic endothelial cells Beilstein Journal of Nanotechnology cell morphology contact guidance microgrooves silicon human aortic endothelial cells (HAECs) |
| title | Silicon microgrooves for contact guidance of human aortic endothelial cells |
| title_full | Silicon microgrooves for contact guidance of human aortic endothelial cells |
| title_fullStr | Silicon microgrooves for contact guidance of human aortic endothelial cells |
| title_full_unstemmed | Silicon microgrooves for contact guidance of human aortic endothelial cells |
| title_short | Silicon microgrooves for contact guidance of human aortic endothelial cells |
| title_sort | silicon microgrooves for contact guidance of human aortic endothelial cells |
| topic | cell morphology contact guidance microgrooves silicon human aortic endothelial cells (HAECs) |
| url | https://doi.org/10.3762/bjnano.8.72 |
| work_keys_str_mv | AT sarafernandezcastillejo siliconmicrogroovesforcontactguidanceofhumanaorticendothelialcells AT pilarformentin siliconmicrogroovesforcontactguidanceofhumanaorticendothelialcells AT ursulacatalan siliconmicrogroovesforcontactguidanceofhumanaorticendothelialcells AT joseppallares siliconmicrogroovesforcontactguidanceofhumanaorticendothelialcells AT lluisfmarsal siliconmicrogroovesforcontactguidanceofhumanaorticendothelialcells AT rosasola siliconmicrogroovesforcontactguidanceofhumanaorticendothelialcells |