Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex
Megavoltage radiotherapy and cisplatin-based chemotherapy are the primary glioblastoma treatments. Novel nanoparticles have been designed to reduce adverse effects and boost therapeutic effectiveness. In the present study, we synthesized the SPIO@AuNP-Cisplatin-Alginate (SACA) nanocomplex, composed...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402301054X |
| _version_ | 1797851901433217024 |
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| author | Mahdie Mousavi Fereshteh Koosha Ali Neshastehriz |
| author_facet | Mahdie Mousavi Fereshteh Koosha Ali Neshastehriz |
| author_sort | Mahdie Mousavi |
| collection | DOAJ |
| description | Megavoltage radiotherapy and cisplatin-based chemotherapy are the primary glioblastoma treatments. Novel nanoparticles have been designed to reduce adverse effects and boost therapeutic effectiveness. In the present study, we synthesized the SPIO@AuNP-Cisplatin-Alginate (SACA) nanocomplex, composed of a SPIO core, a gold shell, and an alginate coating. SACA was characterized using transmission electron microscopy (TEM) and dynamic light scattering (DLS). U87-MG human glioblastoma cells and the HGF cell line (a healthy primary gingival fibroblast) were treated in multiple groups by a combination of SACA, cisplatin, and 6 MV X-ray. The MTT assay was used to assess the cytotoxicity of cisplatin and SACA (at various concentrations and for 4 h). Following the treatments, apoptosis and cell viability were evaluated in each treatment group using flow cytometry and the MTT assay, respectively. The findings demonstrated that the combination of SACA and 6 MV X-rays (at the doses of 2 and 4 Gy) drastically decreased the viability of U87MG cells, whereas the viability of HGF cells remained unchanged. Moreover, U87MG cells treated with SACA in combination with radiation exhibited a significant increase in apoptosis, demonstrating that this nanocomplex effectively boosted the radiosensitivity of cancer cells. Even though additional in vivo studies are needed, these findings suggest that SACA might be used as a radiosensitizer nanoparticle in the therapy of brain tumors. |
| first_indexed | 2024-04-09T19:25:20Z |
| format | Article |
| id | doaj.art-30ebcc5b09a84889a50d30f3681686bc |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:25:20Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-30ebcc5b09a84889a50d30f3681686bc2023-04-05T08:17:27ZengElsevierHeliyon2405-84402023-03-0193e13847Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplexMahdie Mousavi0Fereshteh Koosha1Ali Neshastehriz2Radiation Biology Research Center, Iran University of Medical Science (IUMS), Tehran, Iran; Radiation Science Department, Iran University of Medical Science (IUMS), Tehran, IranDepartment of Radiology Technology, school of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author. Department of Radiology Technology, Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Darband St, Ghods Sq., Tehran, Iran.Radiation Biology Research Center, Iran University of Medical Science (IUMS), Tehran, Iran; Radiation Science Department, Iran University of Medical Science (IUMS), Tehran, Iran; Corresponding author. Radiation Science Department, Iran University of Medical Sciences, Iran.Megavoltage radiotherapy and cisplatin-based chemotherapy are the primary glioblastoma treatments. Novel nanoparticles have been designed to reduce adverse effects and boost therapeutic effectiveness. In the present study, we synthesized the SPIO@AuNP-Cisplatin-Alginate (SACA) nanocomplex, composed of a SPIO core, a gold shell, and an alginate coating. SACA was characterized using transmission electron microscopy (TEM) and dynamic light scattering (DLS). U87-MG human glioblastoma cells and the HGF cell line (a healthy primary gingival fibroblast) were treated in multiple groups by a combination of SACA, cisplatin, and 6 MV X-ray. The MTT assay was used to assess the cytotoxicity of cisplatin and SACA (at various concentrations and for 4 h). Following the treatments, apoptosis and cell viability were evaluated in each treatment group using flow cytometry and the MTT assay, respectively. The findings demonstrated that the combination of SACA and 6 MV X-rays (at the doses of 2 and 4 Gy) drastically decreased the viability of U87MG cells, whereas the viability of HGF cells remained unchanged. Moreover, U87MG cells treated with SACA in combination with radiation exhibited a significant increase in apoptosis, demonstrating that this nanocomplex effectively boosted the radiosensitivity of cancer cells. Even though additional in vivo studies are needed, these findings suggest that SACA might be used as a radiosensitizer nanoparticle in the therapy of brain tumors.http://www.sciencedirect.com/science/article/pii/S240584402301054XRadiotherapyChemotherapyCisplatinGold nanoparticleGlioma |
| spellingShingle | Mahdie Mousavi Fereshteh Koosha Ali Neshastehriz Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex Heliyon Radiotherapy Chemotherapy Cisplatin Gold nanoparticle Glioma |
| title | Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex |
| title_full | Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex |
| title_fullStr | Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex |
| title_full_unstemmed | Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex |
| title_short | Chemo-radiation therapy of U87-MG glioblastoma cells using SPIO@AuNP-Cisplatin-Alginate nanocomplex |
| title_sort | chemo radiation therapy of u87 mg glioblastoma cells using spio aunp cisplatin alginate nanocomplex |
| topic | Radiotherapy Chemotherapy Cisplatin Gold nanoparticle Glioma |
| url | http://www.sciencedirect.com/science/article/pii/S240584402301054X |
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