Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon
Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2009-12-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916302546 |
| _version_ | 1819090550649782272 |
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| author | BUDI SETIADI DARYONO KENJI WAKUI KEIKO T. NATSUAKI |
| author_facet | BUDI SETIADI DARYONO KENJI WAKUI KEIKO T. NATSUAKI |
| author_sort | BUDI SETIADI DARYONO |
| collection | DOAJ |
| description | Two random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. |
| first_indexed | 2024-12-21T22:25:37Z |
| format | Article |
| id | doaj.art-30ec3ce82c9b46bd94cc0b3ff8da5731 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-21T22:25:37Z |
| publishDate | 2009-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-30ec3ce82c9b46bd94cc0b3ff8da57312022-12-21T18:48:13ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192009-12-0116414214610.4308/hjb.16.4.142Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in MelonBUDI SETIADI DARYONO0KENJI WAKUI1KEIKO T. NATSUAKI2Genetics Laboratory, Faculty of Biology, Gadjah Mada University, Yogyakarta 55281, IndonesiaLaboratory of Genetics and Plant Breeding, Department of Bioproduction Technology, Tokyo University of Agriculture, Tokyo 156-8502, JapanLaboratory of Tropical Plant Protection, Graduate School of Agriculture, Tokyo University of Agriculture, Tokyo 156-8502, JapanTwo random amplified polymorphic DNA (RAPD) markers linked to CMV-B2 resistance gene (Creb-2) in melon cultivar Yamatouri were cloned and sequenced to design sequence characterized amplified region (SCAR) markers for detection of CMV-B2 resistance gene (Creb-2) in melon. SCOPE14 derived from OPE-14 yielded a single DNA band at 541 bp, while SCAPB05 derived from APB-05, yielded a single DNA band at 1,046 bp, respectively. Segregation of SCOPE14 and SCAPB05 markers in bulk of F2 plants demonstrated that they were co-segregated with RAPD markers from which the SCAR markers were originated. Furthermore, results of SCAR analysis in diverse melons showed SCAPB05 primers obtained a single 1,046 bp linked to Creb-2 in resistant cultivars Sanuki-shirouri and Kohimeuri. However, SCOPE14 failed to detect Creb-2 in diverse melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to CMV-B2 by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.http://www.sciencedirect.com/science/article/pii/S1978301916302546Cucumis melo L.Creb-2RAPDSCAR |
| spellingShingle | BUDI SETIADI DARYONO KENJI WAKUI KEIKO T. NATSUAKI Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon Hayati Journal of Biosciences Cucumis melo L. Creb-2 RAPD SCAR |
| title | Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon |
| title_full | Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon |
| title_fullStr | Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon |
| title_full_unstemmed | Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon |
| title_short | Development of Random Amplified Polymorphism DNA Markers Linked to CMV-B2 Resistance Gene in Melon |
| title_sort | development of random amplified polymorphism dna markers linked to cmv b2 resistance gene in melon |
| topic | Cucumis melo L. Creb-2 RAPD SCAR |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916302546 |
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