Morphological evidence for phages in <it>Xylella fastidiosa</it>
<p>Abstract</p> <p>Presumptive phage particles associated with <it>Xylella fastidiosa </it>strain Temecula-1 grown in PW broth were observed by transmission electron microscopy (TEM) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and...
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| Format: | Article |
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BMC
2008-06-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/5/1/75 |
| _version_ | 1818662957446332416 |
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| author | Civerolo Edwin L Chen Jianchi |
| author_facet | Civerolo Edwin L Chen Jianchi |
| author_sort | Civerolo Edwin L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Presumptive phage particles associated with <it>Xylella fastidiosa </it>strain Temecula-1 grown in PW broth were observed by transmission electron microscopy (TEM) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and in partially purified preparations from CsCl equilibrium centrifugation density gradients. Ultrathin-sectioned cell pellets contained icosahedral particles of about 45 nm in diameter. Samples collected from CsCl density gradients revealed mostly non-tailed icosahedral but also tailed particles. The icosahedral particles could be divided into two types: a large type (about 45 nm) and a small type (about 30 nm). Filamentous phage-like particles (17 × 120 to 6,300 nm) were also observed. The presence of different types of phage-like particles resembling to those in several bacteriophage families provides new physical evidence, in addition to <it>X. fastidiosa </it>genomic information, that <it>X. fastidiosa </it>possesses active phages. This is the first report of phage particles released in <it>X. fastidiosa </it>cultures.</p> |
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| format | Article |
| id | doaj.art-30ec448c6d8b4bf2a17979ebe49d67f3 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-17T05:09:12Z |
| publishDate | 2008-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-30ec448c6d8b4bf2a17979ebe49d67f32022-12-21T22:02:18ZengBMCVirology Journal1743-422X2008-06-01517510.1186/1743-422X-5-75Morphological evidence for phages in <it>Xylella fastidiosa</it>Civerolo Edwin LChen Jianchi<p>Abstract</p> <p>Presumptive phage particles associated with <it>Xylella fastidiosa </it>strain Temecula-1 grown in PW broth were observed by transmission electron microscopy (TEM) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and in partially purified preparations from CsCl equilibrium centrifugation density gradients. Ultrathin-sectioned cell pellets contained icosahedral particles of about 45 nm in diameter. Samples collected from CsCl density gradients revealed mostly non-tailed icosahedral but also tailed particles. The icosahedral particles could be divided into two types: a large type (about 45 nm) and a small type (about 30 nm). Filamentous phage-like particles (17 × 120 to 6,300 nm) were also observed. The presence of different types of phage-like particles resembling to those in several bacteriophage families provides new physical evidence, in addition to <it>X. fastidiosa </it>genomic information, that <it>X. fastidiosa </it>possesses active phages. This is the first report of phage particles released in <it>X. fastidiosa </it>cultures.</p>http://www.virologyj.com/content/5/1/75 |
| spellingShingle | Civerolo Edwin L Chen Jianchi Morphological evidence for phages in <it>Xylella fastidiosa</it> Virology Journal |
| title | Morphological evidence for phages in <it>Xylella fastidiosa</it> |
| title_full | Morphological evidence for phages in <it>Xylella fastidiosa</it> |
| title_fullStr | Morphological evidence for phages in <it>Xylella fastidiosa</it> |
| title_full_unstemmed | Morphological evidence for phages in <it>Xylella fastidiosa</it> |
| title_short | Morphological evidence for phages in <it>Xylella fastidiosa</it> |
| title_sort | morphological evidence for phages in it xylella fastidiosa it |
| url | http://www.virologyj.com/content/5/1/75 |
| work_keys_str_mv | AT civeroloedwinl morphologicalevidenceforphagesinitxylellafastidiosait AT chenjianchi morphologicalevidenceforphagesinitxylellafastidiosait |