The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant
Background: Polyomavirus BK is one of the life-threatening infections in kidney transplant recipients. The activation of this virus can eventually lead to the loss of the graft. There are very few studies on the expression level of BK virus microRNAs. Therefore, in this study, we investigated the fu...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bushehr University of Medical Sciences
2024-03-01
|
| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/article-1-1857-en.pdf |
| _version_ | 1797230038832119808 |
|---|---|
| author | Razeah Sepahi Eisa Jorjani Afsoon Afshari Hossein Sabouri Ramin Yaghobi |
| author_facet | Razeah Sepahi Eisa Jorjani Afsoon Afshari Hossein Sabouri Ramin Yaghobi |
| author_sort | Razeah Sepahi |
| collection | DOAJ |
| description | Background: Polyomavirus BK is one of the life-threatening infections in kidney transplant recipients. The activation of this virus can eventually lead to the loss of the graft. There are very few studies on the expression level of BK virus microRNAs. Therefore, in this study, we investigated the function of BK virus microRNAs and their effect on host cell microRNAs’ expression level. BK virus encodes two microRNAs, namely miR-B1-3p and miR-B1-5p. Therefore, it is crucial to study these microRNAs as markers of viral infection and their regulation. So far, there is no approved drug or vaccine to treat BK virus infection. Consequently, understanding the relationship between BK virus and host cell microRNAs is integral to the control of infection in kidney transplant patients.
Materials and Methods: In this study, ten tissue samples from kidney transplant recipients with symptoms of BK virus nephropathy, ten urine samples from kidney transplant patients without active BK virus infection, and 20 healthy individuals were included. The expression level of the studied microRNAs was measured in all the samples using SYBR Green Real-time PCR.
Results: The results showed that the expression level of the studied microRNAs, including miR-B1-3p, miR-B1-5p, miR-155, miR-520, miR-10b, miR-30a in the tissue samples of kidney transplant patients with BK virus nephropathy symptoms were significantly increased compared to kidney transplant patients without active BK virus infection.
Conclusion: The assessment of some microRNAs, such as miR-520, miR-30a, and miR-B1-3p/5p, may assist in the prediction and prevention of BKPyV activation in KTRs, particularly in urine samples. |
| first_indexed | 2024-04-24T15:22:08Z |
| format | Article |
| id | doaj.art-30ed4649dede4c689b6f82a26f7b2a02 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-04-24T15:22:08Z |
| publishDate | 2024-03-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-30ed4649dede4c689b6f82a26f7b2a022024-04-02T07:32:42ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542024-03-01264212223The Effect of BK Virus and Host Cell MicroRNAs in Kidney TransplantRazeah Sepahi0Eisa Jorjani1Afsoon Afshari2Hossein Sabouri3Ramin Yaghobi4 Department of Biology, School of Science, Gonbad Kavous University, Gonbad-E Kavous, Iran Department of Biology, School of Science, Gonbad Kavous University, Gonbad-E Kavous, Iran Shiraz Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Plant Production, School of Agriculture Science and Natural Resources, Gonbad Kavous University, Gonbad-E Kavous, Iran Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Background: Polyomavirus BK is one of the life-threatening infections in kidney transplant recipients. The activation of this virus can eventually lead to the loss of the graft. There are very few studies on the expression level of BK virus microRNAs. Therefore, in this study, we investigated the function of BK virus microRNAs and their effect on host cell microRNAs’ expression level. BK virus encodes two microRNAs, namely miR-B1-3p and miR-B1-5p. Therefore, it is crucial to study these microRNAs as markers of viral infection and their regulation. So far, there is no approved drug or vaccine to treat BK virus infection. Consequently, understanding the relationship between BK virus and host cell microRNAs is integral to the control of infection in kidney transplant patients. Materials and Methods: In this study, ten tissue samples from kidney transplant recipients with symptoms of BK virus nephropathy, ten urine samples from kidney transplant patients without active BK virus infection, and 20 healthy individuals were included. The expression level of the studied microRNAs was measured in all the samples using SYBR Green Real-time PCR. Results: The results showed that the expression level of the studied microRNAs, including miR-B1-3p, miR-B1-5p, miR-155, miR-520, miR-10b, miR-30a in the tissue samples of kidney transplant patients with BK virus nephropathy symptoms were significantly increased compared to kidney transplant patients without active BK virus infection. Conclusion: The assessment of some microRNAs, such as miR-520, miR-30a, and miR-B1-3p/5p, may assist in the prediction and prevention of BKPyV activation in KTRs, particularly in urine samples.http://ismj.bpums.ac.ir/article-1-1857-en.pdfkidney transplantbk virusmicro rnanephropathymir-b1-3pmir-b1-5p |
| spellingShingle | Razeah Sepahi Eisa Jorjani Afsoon Afshari Hossein Sabouri Ramin Yaghobi The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant Iranian South Medical Journal kidney transplant bk virus micro rna nephropathy mir-b1-3p mir-b1-5p |
| title | The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant |
| title_full | The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant |
| title_fullStr | The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant |
| title_full_unstemmed | The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant |
| title_short | The Effect of BK Virus and Host Cell MicroRNAs in Kidney Transplant |
| title_sort | effect of bk virus and host cell micrornas in kidney transplant |
| topic | kidney transplant bk virus micro rna nephropathy mir-b1-3p mir-b1-5p |
| url | http://ismj.bpums.ac.ir/article-1-1857-en.pdf |
| work_keys_str_mv | AT razeahsepahi theeffectofbkvirusandhostcellmicrornasinkidneytransplant AT eisajorjani theeffectofbkvirusandhostcellmicrornasinkidneytransplant AT afsoonafshari theeffectofbkvirusandhostcellmicrornasinkidneytransplant AT hosseinsabouri theeffectofbkvirusandhostcellmicrornasinkidneytransplant AT raminyaghobi theeffectofbkvirusandhostcellmicrornasinkidneytransplant AT razeahsepahi effectofbkvirusandhostcellmicrornasinkidneytransplant AT eisajorjani effectofbkvirusandhostcellmicrornasinkidneytransplant AT afsoonafshari effectofbkvirusandhostcellmicrornasinkidneytransplant AT hosseinsabouri effectofbkvirusandhostcellmicrornasinkidneytransplant AT raminyaghobi effectofbkvirusandhostcellmicrornasinkidneytransplant |