FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation
FT-IR/ATR analytical technique is one of the most applicable techniques worldwide. It is closely associated with easy-to-use equipment, rapid analysis, and reliable results. This study reports the simultaneous qualitative and quantitative analysis of two active pharmaceutical ingredients (APIs), of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/24/6051 |
| _version_ | 1797543903241437184 |
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| author | Ioanna Chrisikou Malvina Orkoula Christos Kontoyannis |
| author_facet | Ioanna Chrisikou Malvina Orkoula Christos Kontoyannis |
| author_sort | Ioanna Chrisikou |
| collection | DOAJ |
| description | FT-IR/ATR analytical technique is one of the most applicable techniques worldwide. It is closely associated with easy-to-use equipment, rapid analysis, and reliable results. This study reports the simultaneous qualitative and quantitative analysis of two active pharmaceutical ingredients (APIs), of a piperacillin and tazobactam formulation using a film formation method. This method requires film formation on the ATR crystal, resulting from solvent evaporation of a small amount of liquid sample. Good contact between the film and the crystal led to the identification of both APIs, although tazobactam was of low content in the formulation mixture. The quantification of the APIs in the commercial mixture was also achieved, using a single calibration line with a correlation coefficient equal to 0.999, not only after film formation but also in the initial dry formulation before reconstitution. The present spectroscopic technique combined with the proposed relatively simple sample treatment outweighs chromatographic protocols already applied, which require specialized staff and are costly, time-consuming, and not environmentally friendly. Taking all the above into consideration, it turns out that such an approach has the potential to be used for off-line quality control procedures in manufacture or, in terms of portable equipment and automated software, anywhere for on-site analysis, even in a hospital workflow. |
| first_indexed | 2024-03-10T13:52:57Z |
| format | Article |
| id | doaj.art-30ef593d8cf84bbdb33556947abf89c2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:52:57Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-30ef593d8cf84bbdb33556947abf89c22023-11-21T01:55:57ZengMDPI AGMolecules1420-30492020-12-012524605110.3390/molecules25246051FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam FormulationIoanna Chrisikou0Malvina Orkoula1Christos Kontoyannis2Department of Pharmacy, University of Patras, University Campus, GR-26504 Rio Achaias, GreeceDepartment of Pharmacy, University of Patras, University Campus, GR-26504 Rio Achaias, GreeceDepartment of Pharmacy, University of Patras, University Campus, GR-26504 Rio Achaias, GreeceFT-IR/ATR analytical technique is one of the most applicable techniques worldwide. It is closely associated with easy-to-use equipment, rapid analysis, and reliable results. This study reports the simultaneous qualitative and quantitative analysis of two active pharmaceutical ingredients (APIs), of a piperacillin and tazobactam formulation using a film formation method. This method requires film formation on the ATR crystal, resulting from solvent evaporation of a small amount of liquid sample. Good contact between the film and the crystal led to the identification of both APIs, although tazobactam was of low content in the formulation mixture. The quantification of the APIs in the commercial mixture was also achieved, using a single calibration line with a correlation coefficient equal to 0.999, not only after film formation but also in the initial dry formulation before reconstitution. The present spectroscopic technique combined with the proposed relatively simple sample treatment outweighs chromatographic protocols already applied, which require specialized staff and are costly, time-consuming, and not environmentally friendly. Taking all the above into consideration, it turns out that such an approach has the potential to be used for off-line quality control procedures in manufacture or, in terms of portable equipment and automated software, anywhere for on-site analysis, even in a hospital workflow.https://www.mdpi.com/1420-3049/25/24/6051FT-IR/ATRsample preparationfilm formationpiperacillintazobactam |
| spellingShingle | Ioanna Chrisikou Malvina Orkoula Christos Kontoyannis FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation Molecules FT-IR/ATR sample preparation film formation piperacillin tazobactam |
| title | FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation |
| title_full | FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation |
| title_fullStr | FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation |
| title_full_unstemmed | FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation |
| title_short | FT-IR/ATR Solid Film Formation: Qualitative and Quantitative Analysis of a Piperacillin-Tazobactam Formulation |
| title_sort | ft ir atr solid film formation qualitative and quantitative analysis of a piperacillin tazobactam formulation |
| topic | FT-IR/ATR sample preparation film formation piperacillin tazobactam |
| url | https://www.mdpi.com/1420-3049/25/24/6051 |
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