Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola
Abstract Background Colletotrichum is a fungal genus in Ascomycota that contain many plant pathogens. Among all Colletotrichum genomes that have been sequenced, C. fructicola contains the largest number of candidate virulence factors, such as plant cell wall degrading enzymes, secondary metabolite (...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4934-0 |
| _version_ | 1818334838518710272 |
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| author | Xiaofei Liang Shengping Shang Qiuyue Dong Bo Wang Rong Zhang Mark L. Gleason Guangyu Sun |
| author_facet | Xiaofei Liang Shengping Shang Qiuyue Dong Bo Wang Rong Zhang Mark L. Gleason Guangyu Sun |
| author_sort | Xiaofei Liang |
| collection | DOAJ |
| description | Abstract Background Colletotrichum is a fungal genus in Ascomycota that contain many plant pathogens. Among all Colletotrichum genomes that have been sequenced, C. fructicola contains the largest number of candidate virulence factors, such as plant cell wall degrading enzymes, secondary metabolite (SM) biosynthetic enzymes, secreted proteinases, and small secreted proteins. Systematic analysis of the expressional patterns of these factors would be an important step toward identifying key virulence determinants. Results In this study, we obtained and compared the global transcriptome profiles of four types of infection-related structures: conidia, appressoria, infected apple leaves, and cellophane infectious hyphae (bulbous hyphae spreading inside cellophane) of C. fructicola. We also compared the expression changes of candidate virulence factors among these structures in a systematic manner. A total of 3189 genes were differentially expressed in at least one pairwise comparison. Genes showing in planta-specific expressional upregulations were enriched with small secreted proteins (SSPs), cytochrome P450s, carbohydrate-active enzymes (CAZYs) and secondary metabolite (SM) synthetases, and included homologs of several known candidate effectors and one SM gene cluster specific to the Colletotrichum genus. In conidia, tens of genes functioning in triacylglycerol biosynthesis showed coordinately expressional upregulation, supporting the viewpoint that C. fructicola builds up lipid droplets as energy reserves. Several phosphate starvation responsive genes were coordinately up-regulated during early plant colonization, indicating a phosphate-limited in planta environment immediately faced by biotrophic infectious hyphae. Conclusion This study systematically analyzes the expression patterns of candidate virulence genes, and reveals biological activities related to the development of several infection-related structures of C. fructicola. Our findings lay a foundation for further dissecting infection mechanisms in Colletotrichum and identifying disease control targets. |
| first_indexed | 2024-12-13T14:13:54Z |
| format | Article |
| id | doaj.art-30ef81cfa9fe4ba19002ce83ca370e97 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-13T14:13:54Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-30ef81cfa9fe4ba19002ce83ca370e972022-12-21T23:42:22ZengBMCBMC Genomics1471-21642018-07-0119112110.1186/s12864-018-4934-0Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicolaXiaofei Liang0Shengping Shang1Qiuyue Dong2Bo Wang3Rong Zhang4Mark L. Gleason5Guangyu Sun6State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F UniversityState Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F UniversityDepartment of Plant Pathology and Microbiology, Iowa State UniversityState Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F UniversityAbstract Background Colletotrichum is a fungal genus in Ascomycota that contain many plant pathogens. Among all Colletotrichum genomes that have been sequenced, C. fructicola contains the largest number of candidate virulence factors, such as plant cell wall degrading enzymes, secondary metabolite (SM) biosynthetic enzymes, secreted proteinases, and small secreted proteins. Systematic analysis of the expressional patterns of these factors would be an important step toward identifying key virulence determinants. Results In this study, we obtained and compared the global transcriptome profiles of four types of infection-related structures: conidia, appressoria, infected apple leaves, and cellophane infectious hyphae (bulbous hyphae spreading inside cellophane) of C. fructicola. We also compared the expression changes of candidate virulence factors among these structures in a systematic manner. A total of 3189 genes were differentially expressed in at least one pairwise comparison. Genes showing in planta-specific expressional upregulations were enriched with small secreted proteins (SSPs), cytochrome P450s, carbohydrate-active enzymes (CAZYs) and secondary metabolite (SM) synthetases, and included homologs of several known candidate effectors and one SM gene cluster specific to the Colletotrichum genus. In conidia, tens of genes functioning in triacylglycerol biosynthesis showed coordinately expressional upregulation, supporting the viewpoint that C. fructicola builds up lipid droplets as energy reserves. Several phosphate starvation responsive genes were coordinately up-regulated during early plant colonization, indicating a phosphate-limited in planta environment immediately faced by biotrophic infectious hyphae. Conclusion This study systematically analyzes the expression patterns of candidate virulence genes, and reveals biological activities related to the development of several infection-related structures of C. fructicola. Our findings lay a foundation for further dissecting infection mechanisms in Colletotrichum and identifying disease control targets.http://link.springer.com/article/10.1186/s12864-018-4934-0GlomerellaLeaf spotGLSColletotrichum fructicolaConidiumTriacylglycerol |
| spellingShingle | Xiaofei Liang Shengping Shang Qiuyue Dong Bo Wang Rong Zhang Mark L. Gleason Guangyu Sun Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola BMC Genomics Glomerella Leaf spot GLS Colletotrichum fructicola Conidium Triacylglycerol |
| title | Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola |
| title_full | Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola |
| title_fullStr | Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola |
| title_full_unstemmed | Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola |
| title_short | Transcriptomic analysis reveals candidate genes regulating development and host interactions of Colletotrichum fructicola |
| title_sort | transcriptomic analysis reveals candidate genes regulating development and host interactions of colletotrichum fructicola |
| topic | Glomerella Leaf spot GLS Colletotrichum fructicola Conidium Triacylglycerol |
| url | http://link.springer.com/article/10.1186/s12864-018-4934-0 |
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