Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease
Infection with EBV has been associated with various inflammatory disorders including inflammatory bowel diseases (IBD). Contribution of this virus to intestinal disease processes has not been assessed. We previously detected that EBV DNA triggers proinflammatory responses via the activation of endos...
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MDPI AG
2021-06-01
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| author | Sirine Andari Hadi Hussein Sukayna Fadlallah Abdo R. Jurjus Margret Shirinian Jana G. Hashash Elias A. Rahal |
| author_facet | Sirine Andari Hadi Hussein Sukayna Fadlallah Abdo R. Jurjus Margret Shirinian Jana G. Hashash Elias A. Rahal |
| author_sort | Sirine Andari |
| collection | DOAJ |
| description | Infection with EBV has been associated with various inflammatory disorders including inflammatory bowel diseases (IBD). Contribution of this virus to intestinal disease processes has not been assessed. We previously detected that EBV DNA triggers proinflammatory responses via the activation of endosomal Toll-like receptor (TLR) signaling. Hence, to examine the colitogenic potential of EBV DNA, we used the dextran sodium sulfate (DSS) mouse colitis model. C57BL/6J mice received either DSS-containing or regular drinking water. Mice were then administered EBV DNA by rectal gavage. Administration of EBV DNA to the DSS-fed mice aggravated colonic disease activity as well as increased the damage to the colon histologic architecture. Moreover, we observed enhanced expression of IL-17A, IFNγ and TNFα in colon tissues from the colitis mice (DSS-treated) given the EBV DNA compared to the other groups. This group also had a marked decrease in expression of the CTLA4 immunoregulatory marker. On the other hand, we observed enhanced expression of endosomal TLRs in colon tissues from the EBV DNA-treated colitis mice. These findings indicate that EBV DNA exacerbates proinflammatory responses in colitis. The ubiquity of EBV in the population indicates that possible similar responses may be of pertinence in a relevant proportion of IBD patients. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T04:43:46Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-30f08e02a0864c008c4e6880af0e11c72023-12-03T13:18:09ZengMDPI AGViruses1999-49152021-06-01137127210.3390/v13071272Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel DiseaseSirine Andari0Hadi Hussein1Sukayna Fadlallah2Abdo R. Jurjus3Margret Shirinian4Jana G. Hashash5Elias A. Rahal6Department of Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut, LebanonDivision of Gastroenterology, American University of Beirut Medical Center, Beirut, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, American University of Beirut, Beirut, LebanonInfection with EBV has been associated with various inflammatory disorders including inflammatory bowel diseases (IBD). Contribution of this virus to intestinal disease processes has not been assessed. We previously detected that EBV DNA triggers proinflammatory responses via the activation of endosomal Toll-like receptor (TLR) signaling. Hence, to examine the colitogenic potential of EBV DNA, we used the dextran sodium sulfate (DSS) mouse colitis model. C57BL/6J mice received either DSS-containing or regular drinking water. Mice were then administered EBV DNA by rectal gavage. Administration of EBV DNA to the DSS-fed mice aggravated colonic disease activity as well as increased the damage to the colon histologic architecture. Moreover, we observed enhanced expression of IL-17A, IFNγ and TNFα in colon tissues from the colitis mice (DSS-treated) given the EBV DNA compared to the other groups. This group also had a marked decrease in expression of the CTLA4 immunoregulatory marker. On the other hand, we observed enhanced expression of endosomal TLRs in colon tissues from the EBV DNA-treated colitis mice. These findings indicate that EBV DNA exacerbates proinflammatory responses in colitis. The ubiquity of EBV in the population indicates that possible similar responses may be of pertinence in a relevant proportion of IBD patients.https://www.mdpi.com/1999-4915/13/7/1272inflammatory bowel diseaseEpstein–Barr virusIL-17AToll-like Receptors |
| spellingShingle | Sirine Andari Hadi Hussein Sukayna Fadlallah Abdo R. Jurjus Margret Shirinian Jana G. Hashash Elias A. Rahal Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease Viruses inflammatory bowel disease Epstein–Barr virus IL-17A Toll-like Receptors |
| title | Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease |
| title_full | Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease |
| title_fullStr | Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease |
| title_full_unstemmed | Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease |
| title_short | Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease |
| title_sort | epstein barr virus dna exacerbates colitis symptoms in a mouse model of inflammatory bowel disease |
| topic | inflammatory bowel disease Epstein–Barr virus IL-17A Toll-like Receptors |
| url | https://www.mdpi.com/1999-4915/13/7/1272 |
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