ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress
Arabidopsis thaliana has 14 abscisic acid (ABA) receptors—PYR1/PYLs/RCARs—which have diverse and redundant functions in ABA signaling; however, the precise role of these ABA receptors remains to be elucidated. Here, we report the functional characterization of RCAR5/PYL11 in response to cold stress....
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.587620/full |
| _version_ | 1828837629318135808 |
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| author | Chae Woo Lim Sung Chul Lee |
| author_facet | Chae Woo Lim Sung Chul Lee |
| author_sort | Chae Woo Lim |
| collection | DOAJ |
| description | Arabidopsis thaliana has 14 abscisic acid (ABA) receptors—PYR1/PYLs/RCARs—which have diverse and redundant functions in ABA signaling; however, the precise role of these ABA receptors remains to be elucidated. Here, we report the functional characterization of RCAR5/PYL11 in response to cold stress. Expression of RCAR5 gene in dry seeds and leaves was ABA-dependent and ABA-independent, respectively. Under cold stress conditions, seed germination was negatively affected by the level of RCAR5 expression, which was dependent on ABA and was regulated by HAB1, OST1, and ABI5—downstream components of RCAR5 in ABA signaling. Leaves of RCAR5-overexpressing plants showed enhanced stomatal closure—independent of ABA—and high expression levels of cold, dehydration, and/or ABA-responsive genes compared to those of wild-type; these traits conferred enhanced freezing tolerance. Our data suggest that RCAR5 functions in response to cold stress by delaying seed germination and inducing rapid stomatal closure via ABA-dependent and ABA-independent pathways, respectively. |
| first_indexed | 2024-12-12T18:45:05Z |
| format | Article |
| id | doaj.art-30f2af98caac4be29ae681ce85c8360f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T18:45:05Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-30f2af98caac4be29ae681ce85c8360f2022-12-22T00:15:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-09-011110.3389/fpls.2020.587620587620ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold StressChae Woo LimSung Chul LeeArabidopsis thaliana has 14 abscisic acid (ABA) receptors—PYR1/PYLs/RCARs—which have diverse and redundant functions in ABA signaling; however, the precise role of these ABA receptors remains to be elucidated. Here, we report the functional characterization of RCAR5/PYL11 in response to cold stress. Expression of RCAR5 gene in dry seeds and leaves was ABA-dependent and ABA-independent, respectively. Under cold stress conditions, seed germination was negatively affected by the level of RCAR5 expression, which was dependent on ABA and was regulated by HAB1, OST1, and ABI5—downstream components of RCAR5 in ABA signaling. Leaves of RCAR5-overexpressing plants showed enhanced stomatal closure—independent of ABA—and high expression levels of cold, dehydration, and/or ABA-responsive genes compared to those of wild-type; these traits conferred enhanced freezing tolerance. Our data suggest that RCAR5 functions in response to cold stress by delaying seed germination and inducing rapid stomatal closure via ABA-dependent and ABA-independent pathways, respectively.https://www.frontiersin.org/article/10.3389/fpls.2020.587620/fullabscisic acidcold stressRCARseed germinationstomatal closure |
| spellingShingle | Chae Woo Lim Sung Chul Lee ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress Frontiers in Plant Science abscisic acid cold stress RCAR seed germination stomatal closure |
| title | ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress |
| title_full | ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress |
| title_fullStr | ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress |
| title_full_unstemmed | ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress |
| title_short | ABA-Dependent and ABA-Independent Functions of RCAR5/PYL11 in Response to Cold Stress |
| title_sort | aba dependent and aba independent functions of rcar5 pyl11 in response to cold stress |
| topic | abscisic acid cold stress RCAR seed germination stomatal closure |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.587620/full |
| work_keys_str_mv | AT chaewoolim abadependentandabaindependentfunctionsofrcar5pyl11inresponsetocoldstress AT sungchullee abadependentandabaindependentfunctionsofrcar5pyl11inresponsetocoldstress |