Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect
In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe<sub>2</sub>O<sub>4</sub>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/3/695 |
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| author | Dana Almohazey Vijaya Ravinayagam Widyan Alamoudi Sultan Akhtar H. Dafalla Hind Nasser AlSuwaidan Shoruq. T. Almutairi Hajer Saleh Alghamdi Sukaina Ahmed Aldamen M. A. Almessiere A. Baykal Ahmed A. Maarouf B. Rabindran Jermy |
| author_facet | Dana Almohazey Vijaya Ravinayagam Widyan Alamoudi Sultan Akhtar H. Dafalla Hind Nasser AlSuwaidan Shoruq. T. Almutairi Hajer Saleh Alghamdi Sukaina Ahmed Aldamen M. A. Almessiere A. Baykal Ahmed A. Maarouf B. Rabindran Jermy |
| author_sort | Dana Almohazey |
| collection | DOAJ |
| description | In nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe<sub>2</sub>O<sub>4</sub>/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe<sub>2</sub>O<sub>4</sub>/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn<sup>2+</sup> species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt<sup>4+</sup> species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe<sub>2</sub>O<sub>4</sub>/silica/cisplatin and MnFe<sub>2</sub>O<sub>4</sub>/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death. |
| first_indexed | 2024-03-11T09:50:51Z |
| format | Article |
| id | doaj.art-30f55ece0d534cb89865d7869228fa47 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T09:50:51Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-30f55ece0d534cb89865d7869228fa472023-11-16T16:15:54ZengMDPI AGCancers2072-66942023-01-0115369510.3390/cancers15030695Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer EffectDana Almohazey0Vijaya Ravinayagam1Widyan Alamoudi2Sultan Akhtar3H. Dafalla4Hind Nasser AlSuwaidan5Shoruq. T. Almutairi6Hajer Saleh Alghamdi7Sukaina Ahmed Aldamen8M. A. Almessiere9A. Baykal10Ahmed A. Maarouf11B. Rabindran Jermy12Department of Stem Cell Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDeanship of Scientific Research & Department of Nano-Medicine Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment of Neuroscience Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment of Biophysics Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box. 1982, Dammam 31441, Saudi ArabiaCore Research Facilities (CRF), King Fahd University of Petroleum and Minerals, Dhahran 31261, Saudi ArabiaPharmD, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaPharmD, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment of Pharmaceutics, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Nano-Medicine Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment of Biophysics Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box. 1982, Dammam 31441, Saudi ArabiaDepartment of Nano-Medicine Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment of Physics, Faculty of Basic Sciences, The German University in Cairo, New Cairo 13411, EgyptDepartment of Nano-Medicine Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaIn nanotherapeutics, gaining insight about the drug interaction with the pore architecture and surface functional groups of nanocarriers is crucial to aid in the development of targeted drug delivery. Manganese ferrite impregnated graphene oxide (MnFe<sub>2</sub>O<sub>4</sub>/GO) with a two-dimensional sheet and spherical silica with a three-dimensional interconnected porous structure (MnFe<sub>2</sub>O<sub>4</sub>/silica) were evaluated for cisplatin release and cytotoxic effects. Characterization studies revealed the presence of Mn<sup>2+</sup> species with a variable spinel cubic phase and superparamagnetic effect. We used first principles calculations to study the physisorption of cisplatin on monodispersed silica and on single- and multi-layered GO. The binding energy of cisplatin on silica and single-layer GO was ~1.5 eV, while it was about double that value for the multilayer GO structure. Moreover, we treated MCF-7 (breast cancer cells) and HFF-1 (human foreskin fibroblast) with our nanocomposites and used the cell viability assay MTT. Both nanocomposites significantly reduced the cell viability. Pt<sup>4+</sup> species of cisplatin on the spinel ferrite/silica nanocomposite had a better effect on the cytotoxic capability when compared to GO. The EC50 for MnFe<sub>2</sub>O<sub>4</sub>/silica/cisplatin and MnFe<sub>2</sub>O<sub>4</sub>/GO/cisplatin on MCF-7 was: 48.43 µg/mL and 85.36 µg/mL, respectively. The EC50 for the same conditions on HFF was: 102.92 µg/mL and 102.21 µg/mL, respectively. In addition, immunofluorescence images using c-caspase 3/7, and TEM analysis indicated that treating cells with these nanocomposites resulted in apoptosis as the major mechanism of cell death.https://www.mdpi.com/2072-6694/15/3/695multifunctionalmanganese ferritecisplatindrug deliverynanotherapeuticsdensity functional theory |
| spellingShingle | Dana Almohazey Vijaya Ravinayagam Widyan Alamoudi Sultan Akhtar H. Dafalla Hind Nasser AlSuwaidan Shoruq. T. Almutairi Hajer Saleh Alghamdi Sukaina Ahmed Aldamen M. A. Almessiere A. Baykal Ahmed A. Maarouf B. Rabindran Jermy Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect Cancers multifunctional manganese ferrite cisplatin drug delivery nanotherapeutics density functional theory |
| title | Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect |
| title_full | Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect |
| title_fullStr | Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect |
| title_full_unstemmed | Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect |
| title_short | Insights of Platinum Drug Interaction with Spinel Magnetic Nanocomposites for Targeted Anti-Cancer Effect |
| title_sort | insights of platinum drug interaction with spinel magnetic nanocomposites for targeted anti cancer effect |
| topic | multifunctional manganese ferrite cisplatin drug delivery nanotherapeutics density functional theory |
| url | https://www.mdpi.com/2072-6694/15/3/695 |
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