Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress
Background: During salt stress, the yeast Debaryomyces hansenii synthesizes tyrosine as a strategy to avoid the oxidation of proteins. Tyrosine reacts with nitrogen radicals to form 3-nitrotyrosine. 3-nitrotyrosine prevents the effects of associated oxidative stress and thus contributes to the high...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345817300350 |
| _version_ | 1818326992878043136 |
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| author | Daniela E. Castro Miguel Murguía-Romero Patricia E. Thomé Antonio Peña Marissa Calderón-Torres |
| author_facet | Daniela E. Castro Miguel Murguía-Romero Patricia E. Thomé Antonio Peña Marissa Calderón-Torres |
| author_sort | Daniela E. Castro |
| collection | DOAJ |
| description | Background: During salt stress, the yeast Debaryomyces hansenii synthesizes tyrosine as a strategy to avoid the oxidation of proteins. Tyrosine reacts with nitrogen radicals to form 3-nitrotyrosine. 3-nitrotyrosine prevents the effects of associated oxidative stress and thus contributes to the high halotolerace of the yeast. However, the mechanism of how D. hansenii counteracts the presence of this toxic compound is unclear. In this work, we evaluated D. hansenii's capacity to assimilate 3-nitrotyrosine as a unique nitrogen source and measured its denitrase activity under salt stress. To identify putative genes related to the assimilation of 3-nitrotyrosine, we performed an in silico search in the promoter regions of D. hansenii genome.
Results: We identified 15 genes whose promoters had binding site sequences for transcriptional factors of sodium, nitrogen, and oxidative stress with oxidoreductase and monooxygenase GO annotations. Two of these genes, DEHA2E24178g and DEHA2C00286g, coding for putative denitrases and having GATA sequences, were evaluated by RT-PCR and showed high expression under salt and nitrogen stress.
Conclusions: D. hansenii can grow in the presence of 3-nitrotyrosine as the only nitrogen source and has a high specific denitrase activity to degrade 3-nitrotyrosine in 1 and 2 M NaCl stress conditions. The results suggest that given the lack of information on transcriptional factors in D. hansenii, the genes identified in our in silico analysis may help explain 3-nitrotyrosine assimilation mechanisms. |
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| id | doaj.art-30f745e0e69543449dc6c26de68d0489 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-13T12:09:11Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-30f745e0e69543449dc6c26de68d04892022-12-21T23:46:52ZengElsevierElectronic Journal of Biotechnology0717-34582017-09-0129C1610.1016/j.ejbt.2017.06.003Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stressDaniela E. Castro0Miguel Murguía-Romero1Patricia E. Thomé2Antonio Peña3Marissa Calderón-Torres4Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior s/n, Ciudad de México, MexicoUnidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Ave de los Barrios #1, Col. Los Reyes Iztacala, Tlalnepantla, Estado de México, MexicoUnidad Académica Puerto Morelos, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Apartado Postal 13, Puerto Morelos, Quintana Roo, MexicoDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito Exterior s/n, Ciudad de México, MexicoUnidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Ave de los Barrios #1, Col. Los Reyes Iztacala, Tlalnepantla, Estado de México, MexicoBackground: During salt stress, the yeast Debaryomyces hansenii synthesizes tyrosine as a strategy to avoid the oxidation of proteins. Tyrosine reacts with nitrogen radicals to form 3-nitrotyrosine. 3-nitrotyrosine prevents the effects of associated oxidative stress and thus contributes to the high halotolerace of the yeast. However, the mechanism of how D. hansenii counteracts the presence of this toxic compound is unclear. In this work, we evaluated D. hansenii's capacity to assimilate 3-nitrotyrosine as a unique nitrogen source and measured its denitrase activity under salt stress. To identify putative genes related to the assimilation of 3-nitrotyrosine, we performed an in silico search in the promoter regions of D. hansenii genome. Results: We identified 15 genes whose promoters had binding site sequences for transcriptional factors of sodium, nitrogen, and oxidative stress with oxidoreductase and monooxygenase GO annotations. Two of these genes, DEHA2E24178g and DEHA2C00286g, coding for putative denitrases and having GATA sequences, were evaluated by RT-PCR and showed high expression under salt and nitrogen stress. Conclusions: D. hansenii can grow in the presence of 3-nitrotyrosine as the only nitrogen source and has a high specific denitrase activity to degrade 3-nitrotyrosine in 1 and 2 M NaCl stress conditions. The results suggest that given the lack of information on transcriptional factors in D. hansenii, the genes identified in our in silico analysis may help explain 3-nitrotyrosine assimilation mechanisms.http://www.sciencedirect.com/science/article/pii/S0717345817300350ExtremophilesFree tyrosineHalotoleranceIrreversible damagesNeutralization of free radicalsNitrogen sourceOsmoregulatory mechanismsOxidative stressSalt-tolerant yeastTranscriptional factors (TF)Tyrosine synthesis |
| spellingShingle | Daniela E. Castro Miguel Murguía-Romero Patricia E. Thomé Antonio Peña Marissa Calderón-Torres Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress Electronic Journal of Biotechnology Extremophiles Free tyrosine Halotolerance Irreversible damages Neutralization of free radicals Nitrogen source Osmoregulatory mechanisms Oxidative stress Salt-tolerant yeast Transcriptional factors (TF) Tyrosine synthesis |
| title | Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress |
| title_full | Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress |
| title_fullStr | Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress |
| title_full_unstemmed | Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress |
| title_short | Putative 3-nitrotyrosine detoxifying genes identified in the yeast Debaryomyces hansenii: In silico search of regulatory sequences responsive to salt and nitrogen stress |
| title_sort | putative 3 nitrotyrosine detoxifying genes identified in the yeast debaryomyces hansenii in silico search of regulatory sequences responsive to salt and nitrogen stress |
| topic | Extremophiles Free tyrosine Halotolerance Irreversible damages Neutralization of free radicals Nitrogen source Osmoregulatory mechanisms Oxidative stress Salt-tolerant yeast Transcriptional factors (TF) Tyrosine synthesis |
| url | http://www.sciencedirect.com/science/article/pii/S0717345817300350 |
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