808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-mali...
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MDPI AG
2021-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/11/1717 |
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| author | Silvia Ravera Nadia Bertola Claudio Pasquale Silvia Bruno Stefano Benedicenti Sara Ferrando Angelina Zekiy Praveen Arany Andrea Amaroli |
| author_facet | Silvia Ravera Nadia Bertola Claudio Pasquale Silvia Bruno Stefano Benedicenti Sara Ferrando Angelina Zekiy Praveen Arany Andrea Amaroli |
| author_sort | Silvia Ravera |
| collection | DOAJ |
| description | Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-malignant cells remain unknown. Thus, the primary endpoint was to assess the safety of PBM treatment parameters on head and neck squamous cell carcinoma (HNSCC) proliferation or survival. The secondary endpoint was to assess any putative anti-cancer effects of PBM treatments. Cell viability, energy metabolism, oxidative stress, and pro- and anti-apoptotic markers expression were investigated on a Human Head and Neck Squamous Cell Carcinoma cellular model (OHSU-974 FAcorr cell line). PBM therapy was administered through the 810 nm diode laser (GaAlAs) device (Garda Laser, 7024 Negrar, Verona, Italy) at the powers of 0, 0.25, 0.50, 0.75, 1.00, or 1.25 W in continuous wave (CW) mode for an exposure time of 60 s with a spot-size of 1 cm<sup>2</sup> and with a distance of 1.86 cm from the cells. Results showed that 810-nm PBM affected oxidative phosphorylation in OHSU-971 FAcorr, causing a metabolic switch to anaerobic glycolysis. In addition, PBM reduced the catalase activity, determining an unbalance between oxidative stress production and the antioxidant defenses, which could stimulate the pro-apoptotic cellular pathways. Our data, at the parameters investigated, suggest the safeness of PBM as a supportive cancer therapy. Pre-clinical and clinical studies are necessary to confirm the in vitro evidence. |
| first_indexed | 2024-03-10T05:41:00Z |
| format | Article |
| id | doaj.art-30f789d67d3a45fb9c306f8e5c421c0b |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T05:41:00Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-30f789d67d3a45fb9c306f8e5c421c0b2023-11-22T22:32:24ZengMDPI AGBiomedicines2227-90592021-11-01911171710.3390/biomedicines9111717808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress ProductionSilvia Ravera0Nadia Bertola1Claudio Pasquale2Silvia Bruno3Stefano Benedicenti4Sara Ferrando5Angelina Zekiy6Praveen Arany7Andrea Amaroli8Department of Experimental Medicine, University of Genoa, 16132 Genoa, ItalyDepartment of Experimental Medicine, University of Genoa, 16132 Genoa, ItalyDepartment of Surgical and Diagnostic Sciences, University of Genoa, 16132 Genoa, ItalyDepartment of Experimental Medicine, University of Genoa, 16132 Genoa, ItalyDepartment of Surgical and Diagnostic Sciences, University of Genoa, 16132 Genoa, ItalyDepartment of Earth, Environmental and Life Sciences, University of Genoa, 16132 Genoa, ItalyDepartment of Orthopedic Dentistry, Faculty of Dentistry, First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaDepartments of Oral Biology, Surgery and Biomedical Engineering, University at Buffalo, Buffalo, NY 14260, USADepartment of Surgical and Diagnostic Sciences, University of Genoa, 16132 Genoa, ItalyPhotobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-malignant cells remain unknown. Thus, the primary endpoint was to assess the safety of PBM treatment parameters on head and neck squamous cell carcinoma (HNSCC) proliferation or survival. The secondary endpoint was to assess any putative anti-cancer effects of PBM treatments. Cell viability, energy metabolism, oxidative stress, and pro- and anti-apoptotic markers expression were investigated on a Human Head and Neck Squamous Cell Carcinoma cellular model (OHSU-974 FAcorr cell line). PBM therapy was administered through the 810 nm diode laser (GaAlAs) device (Garda Laser, 7024 Negrar, Verona, Italy) at the powers of 0, 0.25, 0.50, 0.75, 1.00, or 1.25 W in continuous wave (CW) mode for an exposure time of 60 s with a spot-size of 1 cm<sup>2</sup> and with a distance of 1.86 cm from the cells. Results showed that 810-nm PBM affected oxidative phosphorylation in OHSU-971 FAcorr, causing a metabolic switch to anaerobic glycolysis. In addition, PBM reduced the catalase activity, determining an unbalance between oxidative stress production and the antioxidant defenses, which could stimulate the pro-apoptotic cellular pathways. Our data, at the parameters investigated, suggest the safeness of PBM as a supportive cancer therapy. Pre-clinical and clinical studies are necessary to confirm the in vitro evidence.https://www.mdpi.com/2227-9059/9/11/1717apoptosisantioxidant defenseslight therapylow-level laser therapyoral canceroxidative phosphorylation |
| spellingShingle | Silvia Ravera Nadia Bertola Claudio Pasquale Silvia Bruno Stefano Benedicenti Sara Ferrando Angelina Zekiy Praveen Arany Andrea Amaroli 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production Biomedicines apoptosis antioxidant defenses light therapy low-level laser therapy oral cancer oxidative phosphorylation |
| title | 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production |
| title_full | 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production |
| title_fullStr | 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production |
| title_full_unstemmed | 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production |
| title_short | 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production |
| title_sort | 808 nm photobiomodulation affects the viability of a head and neck squamous carcinoma cellular model acting on energy metabolism and oxidative stress production |
| topic | apoptosis antioxidant defenses light therapy low-level laser therapy oral cancer oxidative phosphorylation |
| url | https://www.mdpi.com/2227-9059/9/11/1717 |
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