The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation
In rodents, the melatonin production by the pineal gland is controlled through adrenergic signaling from the suprachiasmatic nuclei and regulation of the principal enzyme in its synthesis, arylalkylamine-N-acetyltransferase (AANAT). In the present study, we identified increased isoprenaline-induced...
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2021-10-01
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| author | Simona Moravcová Eva Filipovská Veronika Spišská Irena Svobodová Jiří Novotný Zdeňka Bendová |
| author_facet | Simona Moravcová Eva Filipovská Veronika Spišská Irena Svobodová Jiří Novotný Zdeňka Bendová |
| author_sort | Simona Moravcová |
| collection | DOAJ |
| description | In rodents, the melatonin production by the pineal gland is controlled through adrenergic signaling from the suprachiasmatic nuclei and regulation of the principal enzyme in its synthesis, arylalkylamine-N-acetyltransferase (AANAT). In the present study, we identified increased isoprenaline-induced <i>aa-nat</i> expression and nocturnal AANAT activity in the pineal glands in response to the silencing of the signal transducer and activator of transcription 3 (STAT3) with siRNA or STAT3 inhibitors WP1066 and AZD1480. This AANAT activity enhancement in vivo did not interfere with light-induced AANAT suppression. Systemic or in vitro lipopolysaccharide (LPS) administration markedly increased <i>Stat3</i> expression and STAT3 phosphorylation, but it did not significantly affect AANAT expression or activity. Simultaneous LPS administration and <i>Stat3</i> silencing enhanced the <i>aa-nat</i> transcription and AANAT activity to a similar extent as <i>Stat3</i> inhibition without LPS co-administration. Furthermore, we describe the circadian rhythmicity in <i>Stat3</i> expression and the phosphorylated form of STAT3 protein in the rat pineal gland. Our data suggest that the higher nocturnal endogenous level of STAT3 in the pineal gland decelerates or hampers the process of NA-induced AANAT activation or affects the AANAT enzyme stability. |
| first_indexed | 2024-03-10T06:26:43Z |
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| id | doaj.art-30f952fd4c964f30b1a693da31932ca8 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T06:26:43Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj.art-30f952fd4c964f30b1a693da31932ca82023-11-22T18:53:40ZengMDPI AGLife2075-17292021-10-011110110510.3390/life11101105The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase RegulationSimona Moravcová0Eva Filipovská1Veronika Spišská2Irena Svobodová3Jiří Novotný4Zdeňka Bendová5Department of Physiology, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicDepartment of Physiology, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicDepartment of Physiology, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicLaboratory of Pain Research, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech RepublicDepartment of Physiology, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicDepartment of Physiology, Faculty of Science, Charles University, 128 43 Prague, Czech RepublicIn rodents, the melatonin production by the pineal gland is controlled through adrenergic signaling from the suprachiasmatic nuclei and regulation of the principal enzyme in its synthesis, arylalkylamine-N-acetyltransferase (AANAT). In the present study, we identified increased isoprenaline-induced <i>aa-nat</i> expression and nocturnal AANAT activity in the pineal glands in response to the silencing of the signal transducer and activator of transcription 3 (STAT3) with siRNA or STAT3 inhibitors WP1066 and AZD1480. This AANAT activity enhancement in vivo did not interfere with light-induced AANAT suppression. Systemic or in vitro lipopolysaccharide (LPS) administration markedly increased <i>Stat3</i> expression and STAT3 phosphorylation, but it did not significantly affect AANAT expression or activity. Simultaneous LPS administration and <i>Stat3</i> silencing enhanced the <i>aa-nat</i> transcription and AANAT activity to a similar extent as <i>Stat3</i> inhibition without LPS co-administration. Furthermore, we describe the circadian rhythmicity in <i>Stat3</i> expression and the phosphorylated form of STAT3 protein in the rat pineal gland. Our data suggest that the higher nocturnal endogenous level of STAT3 in the pineal gland decelerates or hampers the process of NA-induced AANAT activation or affects the AANAT enzyme stability.https://www.mdpi.com/2075-1729/11/10/1105STAT3arylalkylamine-N-acetyltransferasepineal glandcircadian rhythmslipopolysacchariderat |
| spellingShingle | Simona Moravcová Eva Filipovská Veronika Spišská Irena Svobodová Jiří Novotný Zdeňka Bendová The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation Life STAT3 arylalkylamine-N-acetyltransferase pineal gland circadian rhythms lipopolysaccharide rat |
| title | The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation |
| title_full | The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation |
| title_fullStr | The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation |
| title_full_unstemmed | The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation |
| title_short | The Circadian Rhythms of STAT3 in the Rat Pineal Gland and Its Involvement in Arylalkylamine-N-Acetyltransferase Regulation |
| title_sort | circadian rhythms of stat3 in the rat pineal gland and its involvement in arylalkylamine n acetyltransferase regulation |
| topic | STAT3 arylalkylamine-N-acetyltransferase pineal gland circadian rhythms lipopolysaccharide rat |
| url | https://www.mdpi.com/2075-1729/11/10/1105 |
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