Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection
Tissue infection typically results from blood transmission or the direct inoculation of bacteria following trauma. The pathogen-induced destruction of tissue prevents antibiotics from penetrating the infected site, and severe inflammation further impairs the efficacy of conventional treatment. The c...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423001606 |
| _version_ | 1797770725024595968 |
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| author | Wang Peilin Peng Ying Wang Renyuan Li Zhuoxuan Yang Zhenwu Zhao Mai Song Jianguo Zhang Hao Yin Gang Lin Lin Lin Haodong |
| author_facet | Wang Peilin Peng Ying Wang Renyuan Li Zhuoxuan Yang Zhenwu Zhao Mai Song Jianguo Zhang Hao Yin Gang Lin Lin Lin Haodong |
| author_sort | Wang Peilin |
| collection | DOAJ |
| description | Tissue infection typically results from blood transmission or the direct inoculation of bacteria following trauma. The pathogen-induced destruction of tissue prevents antibiotics from penetrating the infected site, and severe inflammation further impairs the efficacy of conventional treatment. The current study describes the size-dependent induction of macrophage polarization using gold nanoparticles. Gold nanoparticles with a diameter of 50 nm (Au50) can induce M2 polarization in macrophages by inhibiting the NF-κB signaling pathway and stimulate an inflammatory response in the environment by inhibiting the MAPK signaling pathway LPS. Furthermore, the induced polarization and anti-inflammatory effects of the Au50 nanoparticles promoted the osteogenic differentiation of BMSCs in vitro. In addition, the overexpression of TREM2 in macrophage induced by Au50 nanoparticles was found to promote macrophage phagocytosis of Staphylococcus aureus, enhance the fusion of autophagosomes and lysosomes, accelerate the intracellular degradation of S. aureus, in addition to achieving an effective local treatment of osteomyelitis and infectious skin defects in conjunction with inflammatory regulation and accelerating bone regeneration. The findings, therefore, demonstrate that Au50 nanoparticles can be utilized as a promising nanomaterial for in vivo treatment of infections. |
| first_indexed | 2024-03-12T21:27:23Z |
| format | Article |
| id | doaj.art-30fae6a648b14ddb8b2a7f6714dfe40e |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-12T21:27:23Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-30fae6a648b14ddb8b2a7f6714dfe40e2023-07-28T04:26:40ZengElsevierMaterials Today Bio2590-00642023-08-0121100700Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infectionWang Peilin0Peng Ying1Wang Renyuan2Li Zhuoxuan3Yang Zhenwu4Zhao Mai5Song Jianguo6Zhang Hao7Yin Gang8Lin Lin9Lin Haodong10Trauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaTrauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, ChinaSchool of Chemical and Environmental, Engineering, Shanghai Institute of Technology, Shanghai 201418, China; Corresponding author.Trauma Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China; Corresponding author.Tissue infection typically results from blood transmission or the direct inoculation of bacteria following trauma. The pathogen-induced destruction of tissue prevents antibiotics from penetrating the infected site, and severe inflammation further impairs the efficacy of conventional treatment. The current study describes the size-dependent induction of macrophage polarization using gold nanoparticles. Gold nanoparticles with a diameter of 50 nm (Au50) can induce M2 polarization in macrophages by inhibiting the NF-κB signaling pathway and stimulate an inflammatory response in the environment by inhibiting the MAPK signaling pathway LPS. Furthermore, the induced polarization and anti-inflammatory effects of the Au50 nanoparticles promoted the osteogenic differentiation of BMSCs in vitro. In addition, the overexpression of TREM2 in macrophage induced by Au50 nanoparticles was found to promote macrophage phagocytosis of Staphylococcus aureus, enhance the fusion of autophagosomes and lysosomes, accelerate the intracellular degradation of S. aureus, in addition to achieving an effective local treatment of osteomyelitis and infectious skin defects in conjunction with inflammatory regulation and accelerating bone regeneration. The findings, therefore, demonstrate that Au50 nanoparticles can be utilized as a promising nanomaterial for in vivo treatment of infections.http://www.sciencedirect.com/science/article/pii/S2590006423001606Au nanoparticlesOsteomyelitisWound infectionStaphylococcus aureusMacrophages polarizationPhagocytosis |
| spellingShingle | Wang Peilin Peng Ying Wang Renyuan Li Zhuoxuan Yang Zhenwu Zhao Mai Song Jianguo Zhang Hao Yin Gang Lin Lin Lin Haodong Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection Materials Today Bio Au nanoparticles Osteomyelitis Wound infection Staphylococcus aureus Macrophages polarization Phagocytosis |
| title | Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection |
| title_full | Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection |
| title_fullStr | Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection |
| title_full_unstemmed | Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection |
| title_short | Size-dependent gold nanoparticles induce macrophage M2 polarization and promote intracellular clearance of Staphylococcus aureus to alleviate tissue infection |
| title_sort | size dependent gold nanoparticles induce macrophage m2 polarization and promote intracellular clearance of staphylococcus aureus to alleviate tissue infection |
| topic | Au nanoparticles Osteomyelitis Wound infection Staphylococcus aureus Macrophages polarization Phagocytosis |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423001606 |
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