Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction
Eukaryotic cells have distinct membrane-enclosed organelles, each with a unique biochemical signature and specialized function. The unique identity of each organelle is greatly governed by the asymmetric distribution and regulated intracellular movement of two important biomolecules, lipids, and pro...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/13/10637 |
| _version_ | 1797591611785347072 |
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| author | Koushik Das Tomoyoshi Nozaki |
| author_facet | Koushik Das Tomoyoshi Nozaki |
| author_sort | Koushik Das |
| collection | DOAJ |
| description | Eukaryotic cells have distinct membrane-enclosed organelles, each with a unique biochemical signature and specialized function. The unique identity of each organelle is greatly governed by the asymmetric distribution and regulated intracellular movement of two important biomolecules, lipids, and proteins. Non-vesicular lipid transport mediated by lipid-transfer proteins (LTPs) plays essential roles in intra-cellular lipid trafficking and cellular lipid homeostasis, while vesicular transport regulates protein trafficking. A comparative analysis of non-vesicular lipid transport machinery in protists could enhance our understanding of parasitism and basis of eukaryotic evolution. <i>Leishmania donovani</i>, the trypanosomatid parasite, greatly depends on receptor-ligand mediated signalling pathways for cellular differentiation, nutrient uptake, secretion of virulence factors, and pathogenesis. Lipids, despite being important signalling molecules, have intracellular transport mechanisms that are largely unexplored in <i>L. donovani</i>. We have identified a repertoire of sixteen (16) potential lipid transfer protein (LTP) homologs based on a domain-based search on TriTrypDB coupled with bioinformatics analyses, which signifies the presence of well-organized lipid transport machinery in this parasite. We emphasized here their evolutionary uniqueness and conservation and discussed their potential implications for parasite biology with regards to future therapeutic targets against visceral leishmaniasis. |
| first_indexed | 2024-03-11T01:39:49Z |
| format | Article |
| id | doaj.art-3100c9c20ce542e7b6c2b95ece5d8263 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T01:39:49Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3100c9c20ce542e7b6c2b95ece5d82632023-11-18T16:41:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131063710.3390/ijms241310637Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite InteractionKoushik Das0Tomoyoshi Nozaki1Department of Allied Health Sciences, School of Health Sciences and Technology, University of Petroleum and Energy Studies, Dehradun 248007, IndiaDepartment of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, JapanEukaryotic cells have distinct membrane-enclosed organelles, each with a unique biochemical signature and specialized function. The unique identity of each organelle is greatly governed by the asymmetric distribution and regulated intracellular movement of two important biomolecules, lipids, and proteins. Non-vesicular lipid transport mediated by lipid-transfer proteins (LTPs) plays essential roles in intra-cellular lipid trafficking and cellular lipid homeostasis, while vesicular transport regulates protein trafficking. A comparative analysis of non-vesicular lipid transport machinery in protists could enhance our understanding of parasitism and basis of eukaryotic evolution. <i>Leishmania donovani</i>, the trypanosomatid parasite, greatly depends on receptor-ligand mediated signalling pathways for cellular differentiation, nutrient uptake, secretion of virulence factors, and pathogenesis. Lipids, despite being important signalling molecules, have intracellular transport mechanisms that are largely unexplored in <i>L. donovani</i>. We have identified a repertoire of sixteen (16) potential lipid transfer protein (LTP) homologs based on a domain-based search on TriTrypDB coupled with bioinformatics analyses, which signifies the presence of well-organized lipid transport machinery in this parasite. We emphasized here their evolutionary uniqueness and conservation and discussed their potential implications for parasite biology with regards to future therapeutic targets against visceral leishmaniasis.https://www.mdpi.com/1422-0067/24/13/10637<i>Leishmania donovani</i>lipid transfer proteinlipid signalingdrug developmentpathogenesis |
| spellingShingle | Koushik Das Tomoyoshi Nozaki Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction International Journal of Molecular Sciences <i>Leishmania donovani</i> lipid transfer protein lipid signaling drug development pathogenesis |
| title | Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction |
| title_full | Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction |
| title_fullStr | Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction |
| title_full_unstemmed | Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction |
| title_short | Non-Vesicular Lipid Transport Machinery in <i>Leishmania donovani</i>: Functional Implications in Host-Parasite Interaction |
| title_sort | non vesicular lipid transport machinery in i leishmania donovani i functional implications in host parasite interaction |
| topic | <i>Leishmania donovani</i> lipid transfer protein lipid signaling drug development pathogenesis |
| url | https://www.mdpi.com/1422-0067/24/13/10637 |
| work_keys_str_mv | AT koushikdas nonvesicularlipidtransportmachineryinileishmaniadonovaniifunctionalimplicationsinhostparasiteinteraction AT tomoyoshinozaki nonvesicularlipidtransportmachineryinileishmaniadonovaniifunctionalimplicationsinhostparasiteinteraction |