Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume
In vitro seed germination has been proven to be the most efficient technique to propagate orchid. The application of this aseptic technique has contributed to conservation of many endangered orchid species. In this study, undehisced capsules of Vanda helvola Blume were collected from Orchid Conserva...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2015-06-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9546 |
| _version_ | 1818506930657689600 |
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| author | Devina DAVID Roslina JAWAN Hartinie MARBAWI Jualang Azlan GANSAU |
| author_facet | Devina DAVID Roslina JAWAN Hartinie MARBAWI Jualang Azlan GANSAU |
| author_sort | Devina DAVID |
| collection | DOAJ |
| description | In vitro seed germination has been proven to be the most efficient technique to propagate orchid. The application of this aseptic technique has contributed to conservation of many endangered orchid species. In this study, undehisced capsules of Vanda helvola Blume were collected from Orchid Conservation Centre in Lagud Sebrang Agriculture Park, after 120 days from hand pollination and aseptically cultured on three types of basal media such as Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW). After 90 days of culture, 66.40 ± 4.14% of seeds successfully germinated on KC medium. The effect of organic additives such as tomato juice, coconut water, peptone and yeast extract at different level of concentrations in KC basal medium were also tested on seed germination and seedling development of this native orchid. After 90 days of culture, over 90% of seeds were tremendously germinated on KC medium supplemented with 10% or 15% (v/v) of tomato juice. The incorporation of peptone at 0.1% (w/v) in KC basal media promoted rapid development of protocorm to seedling. Seedlings on this treatment produced an average of three leaves and two roots after 90 days of culture and were successfully acclimatized. |
| first_indexed | 2024-12-10T22:11:24Z |
| format | Article |
| id | doaj.art-310336035a0e4f04aff41893cbe4bd7c |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-10T22:11:24Z |
| publishDate | 2015-06-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-310336035a0e4f04aff41893cbe4bd7c2022-12-22T01:31:35ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642015-06-017219219710.15835/nsb7295468138Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> BlumeDevina DAVID0Roslina JAWAN1Hartinie MARBAWI2Jualang Azlan GANSAU3University Malaysia Sabah, Faculty of Sustainable Agriculture, Locked Bag No.3, 90509 Sandakan SabahUniversity Malaysia Sabah, Faculty of Science and Natural Resources, UMS Road, 88450 Kota Kinabalu SabahUniversity Malaysia Sabah, Faculty of Science and Natural Resources, UMS Road, 88450 Kota Kinabalu SabahUniversity Malaysia Sabah, Faculty of Science and Natural Resources, UMS Road, 88450 Kota Kinabalu SabahIn vitro seed germination has been proven to be the most efficient technique to propagate orchid. The application of this aseptic technique has contributed to conservation of many endangered orchid species. In this study, undehisced capsules of Vanda helvola Blume were collected from Orchid Conservation Centre in Lagud Sebrang Agriculture Park, after 120 days from hand pollination and aseptically cultured on three types of basal media such as Murashige and Skoog (MS), Knudson C (KC) and Vacin and Went (VW). After 90 days of culture, 66.40 ± 4.14% of seeds successfully germinated on KC medium. The effect of organic additives such as tomato juice, coconut water, peptone and yeast extract at different level of concentrations in KC basal medium were also tested on seed germination and seedling development of this native orchid. After 90 days of culture, over 90% of seeds were tremendously germinated on KC medium supplemented with 10% or 15% (v/v) of tomato juice. The incorporation of peptone at 0.1% (w/v) in KC basal media promoted rapid development of protocorm to seedling. Seedlings on this treatment produced an average of three leaves and two roots after 90 days of culture and were successfully acclimatized.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9546 |
| spellingShingle | Devina DAVID Roslina JAWAN Hartinie MARBAWI Jualang Azlan GANSAU Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume Notulae Scientia Biologicae |
| title | Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume |
| title_full | Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume |
| title_fullStr | Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume |
| title_full_unstemmed | Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume |
| title_short | Organic Additives Improves the <i>in Vitro</i> Growth of Native Orchid <i>Vanda helvola</i> Blume |
| title_sort | organic additives improves the lt i gt in vitro lt i gt growth of native orchid lt i gt vanda helvola lt i gt blume |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9546 |
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