Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806
Gene disruption in cyanobacteria is difficult and comprises an obstacle for genetic manipulation. Very few reports tackled this problem but the methods used are usually obscure and hardly reproducible. Here we describe an optimized electroporation-induced transformation in Microcystis aeruginosa PCC...
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
2010-01-01
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| Series: | Biotechnologie, Agronomie, Société et Environnement |
| Subjects: | |
| Online Access: | http://www.pressesagro.be/base/text/v14n1/149.pdf |
| _version_ | 1811247288798937088 |
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| author | El Semary, A. |
| author_facet | El Semary, A. |
| author_sort | El Semary, A. |
| collection | DOAJ |
| description | Gene disruption in cyanobacteria is difficult and comprises an obstacle for genetic manipulation. Very few reports tackled this problem but the methods used are usually obscure and hardly reproducible. Here we describe an optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 where conditions for successful electroporation and transformation are investigated. |
| first_indexed | 2024-04-12T15:07:27Z |
| format | Article |
| id | doaj.art-310414ced03e42ffa2fd9b55c20b1dc5 |
| institution | Directory Open Access Journal |
| issn | 1370-6233 1780-4507 |
| language | English |
| last_indexed | 2024-04-12T15:07:27Z |
| publishDate | 2010-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Biotechnologie, Agronomie, Société et Environnement |
| spelling | doaj.art-310414ced03e42ffa2fd9b55c20b1dc52022-12-22T03:27:53ZengPresses Agronomiques de GemblouxBiotechnologie, Agronomie, Société et Environnement1370-62331780-45072010-01-01141149152Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806El Semary, A.Gene disruption in cyanobacteria is difficult and comprises an obstacle for genetic manipulation. Very few reports tackled this problem but the methods used are usually obscure and hardly reproducible. Here we describe an optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 where conditions for successful electroporation and transformation are investigated.http://www.pressesagro.be/base/text/v14n1/149.pdfGene disruptionhomologous recombinationelectroporationtransformationMicrocystis aeruginosa PCC7806HPLC |
| spellingShingle | El Semary, A. Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 Biotechnologie, Agronomie, Société et Environnement Gene disruption homologous recombination electroporation transformation Microcystis aeruginosa PCC7806 HPLC |
| title | Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 |
| title_full | Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 |
| title_fullStr | Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 |
| title_full_unstemmed | Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 |
| title_short | Optimized electroporation-induced transformation in Microcystis aeruginosa PCC7806 |
| title_sort | optimized electroporation induced transformation in microcystis aeruginosa pcc7806 |
| topic | Gene disruption homologous recombination electroporation transformation Microcystis aeruginosa PCC7806 HPLC |
| url | http://www.pressesagro.be/base/text/v14n1/149.pdf |
| work_keys_str_mv | AT elsemarya optimizedelectroporationinducedtransformationinmicrocystisaeruginosapcc7806 |