Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme
Salt stress constitutes a major form of abiotic stress in plants. Histone modification plays an important role in stress tolerance, with particular reference to salt stress resistance. In the current study, we found that HDA15 overexpression confers salt stress resistance to young seedling stages of...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.640443/full |
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| author | Hai An Truong Seokjin Lee Cao Son Trịnh Won Je Lee Eui-Hwan Chung Suk-Whan Hong Hojoung Lee |
| author_facet | Hai An Truong Seokjin Lee Cao Son Trịnh Won Je Lee Eui-Hwan Chung Suk-Whan Hong Hojoung Lee |
| author_sort | Hai An Truong |
| collection | DOAJ |
| description | Salt stress constitutes a major form of abiotic stress in plants. Histone modification plays an important role in stress tolerance, with particular reference to salt stress resistance. In the current study, we found that HDA15 overexpression confers salt stress resistance to young seedling stages of transgenic plants. Furthermore, salt stress induces HDA15 overexpression. Transcription levels of stress-responsive genes were increased in transgenic plants overexpressing HDA15 (HDA15 OE). NCED3, an abscisic acid (ABA) biosynthetic gene, which is highly upregulated in HDA15 transgenic plants, enhanced the accumulation of ABA, which promotes adaptation to salt stress. ABA homeostasis in HDA15 OE plants is maintained by the induction of CYP707As, which optimize endogenous ABA levels. Lastly, we found that the double-mutant HDA15 OE/hy5 ko plants are sensitive to salt stress, indicating that interaction between HDA15 and ELONGATED HYPOCOTYL 5 (HY5) is crucial to salt stress tolerance shown by HDA15 OE plants. Thus, our findings indicate that HDA15 is crucial to salt stress tolerance in Arabidopsis. |
| first_indexed | 2024-12-20T06:04:12Z |
| format | Article |
| id | doaj.art-31055a9b05f74b32bbcb141a9c826cb0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T06:04:12Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-31055a9b05f74b32bbcb141a9c826cb02022-12-21T19:50:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.640443640443Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis EnzymeHai An Truong0Seokjin Lee1Cao Son Trịnh2Won Je Lee3Eui-Hwan Chung4Suk-Whan Hong5Hojoung Lee6Department of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaDepartment of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju, South KoreaDepartment of Plant Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, South KoreaSalt stress constitutes a major form of abiotic stress in plants. Histone modification plays an important role in stress tolerance, with particular reference to salt stress resistance. In the current study, we found that HDA15 overexpression confers salt stress resistance to young seedling stages of transgenic plants. Furthermore, salt stress induces HDA15 overexpression. Transcription levels of stress-responsive genes were increased in transgenic plants overexpressing HDA15 (HDA15 OE). NCED3, an abscisic acid (ABA) biosynthetic gene, which is highly upregulated in HDA15 transgenic plants, enhanced the accumulation of ABA, which promotes adaptation to salt stress. ABA homeostasis in HDA15 OE plants is maintained by the induction of CYP707As, which optimize endogenous ABA levels. Lastly, we found that the double-mutant HDA15 OE/hy5 ko plants are sensitive to salt stress, indicating that interaction between HDA15 and ELONGATED HYPOCOTYL 5 (HY5) is crucial to salt stress tolerance shown by HDA15 OE plants. Thus, our findings indicate that HDA15 is crucial to salt stress tolerance in Arabidopsis.https://www.frontiersin.org/articles/10.3389/fpls.2021.640443/fullArabidopsisHDA15 genesalinitystress tolerancehistone modificationABA accumulation |
| spellingShingle | Hai An Truong Seokjin Lee Cao Son Trịnh Won Je Lee Eui-Hwan Chung Suk-Whan Hong Hojoung Lee Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme Frontiers in Plant Science Arabidopsis HDA15 gene salinity stress tolerance histone modification ABA accumulation |
| title | Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme |
| title_full | Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme |
| title_fullStr | Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme |
| title_full_unstemmed | Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme |
| title_short | Overexpression of the HDA15 Gene Confers Resistance to Salt Stress by the Induction of NCED3, an ABA Biosynthesis Enzyme |
| title_sort | overexpression of the hda15 gene confers resistance to salt stress by the induction of nced3 an aba biosynthesis enzyme |
| topic | Arabidopsis HDA15 gene salinity stress tolerance histone modification ABA accumulation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.640443/full |
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