Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift
Abstract A previous study suggested that the airlift condition is superior to the Optisol-GS condition for preserving the limbal tissue of the human cornea. The purpose of this research is to investigate a new preservation device that preserves the cornea while separating epithelial and endothelial...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34039-5 |
| _version_ | 1797774205620584448 |
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| author | Diya Tang Masafumi Uematsu Kohei Harada Yasser Helmy Mohamed Mao Kusano Daisuke Inoue Takashi Kitaoka |
| author_facet | Diya Tang Masafumi Uematsu Kohei Harada Yasser Helmy Mohamed Mao Kusano Daisuke Inoue Takashi Kitaoka |
| author_sort | Diya Tang |
| collection | DOAJ |
| description | Abstract A previous study suggested that the airlift condition is superior to the Optisol-GS condition for preserving the limbal tissue of the human cornea. The purpose of this research is to investigate a new preservation device that preserves the cornea while separating epithelial and endothelial areas. The differences after preserving the corneal epithelium under different conditions were compared. A total of 24 corneas of New Zealand rabbits were divided into four groups in which the corneal epithelia were submersed in Optisol-GS or under airlift conditions for 1 and 2 weeks at 4 $$^{\circ }$$ ∘ C. Transparency, optical coherence tomography (OCT), hematoxylin and eosin (H &E) staining, and epithelial migration tests were used to assess corneal status. The epithelial migration examination showed significantly greater migration ability after the airlift condition. Corneas in the 1-week Optisol-GS group were the most transparent, followed by the 1-week airlift group. OCT showed a progressive increase in corneal thickness to the end of the study. H &E staining showed that the epithelial cells retained intact cellular structure and morphology of the cells for both 1-week-preserved groups. However, there was disruption of the corneal epithelial cell structure for both 2-week-preserved groups. Corneal epithelium preserved under the hypothermic airlift condition was comparable to that under the Optisol-GS condition. |
| first_indexed | 2024-03-12T22:17:39Z |
| format | Article |
| id | doaj.art-3109216875904e0ea66a97f8b61219e3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:17:39Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3109216875904e0ea66a97f8b61219e32023-07-23T11:13:02ZengNature PortfolioScientific Reports2045-23222023-04-011311810.1038/s41598-023-34039-5Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airliftDiya Tang0Masafumi Uematsu1Kohei Harada2Yasser Helmy Mohamed3Mao Kusano4Daisuke Inoue5Takashi Kitaoka6Department of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityDepartment of Ophthalmology and Visual Sciences, Graduate School of Biomedical Sciences, Nagasaki UniversityAbstract A previous study suggested that the airlift condition is superior to the Optisol-GS condition for preserving the limbal tissue of the human cornea. The purpose of this research is to investigate a new preservation device that preserves the cornea while separating epithelial and endothelial areas. The differences after preserving the corneal epithelium under different conditions were compared. A total of 24 corneas of New Zealand rabbits were divided into four groups in which the corneal epithelia were submersed in Optisol-GS or under airlift conditions for 1 and 2 weeks at 4 $$^{\circ }$$ ∘ C. Transparency, optical coherence tomography (OCT), hematoxylin and eosin (H &E) staining, and epithelial migration tests were used to assess corneal status. The epithelial migration examination showed significantly greater migration ability after the airlift condition. Corneas in the 1-week Optisol-GS group were the most transparent, followed by the 1-week airlift group. OCT showed a progressive increase in corneal thickness to the end of the study. H &E staining showed that the epithelial cells retained intact cellular structure and morphology of the cells for both 1-week-preserved groups. However, there was disruption of the corneal epithelial cell structure for both 2-week-preserved groups. Corneal epithelium preserved under the hypothermic airlift condition was comparable to that under the Optisol-GS condition.https://doi.org/10.1038/s41598-023-34039-5 |
| spellingShingle | Diya Tang Masafumi Uematsu Kohei Harada Yasser Helmy Mohamed Mao Kusano Daisuke Inoue Takashi Kitaoka Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift Scientific Reports |
| title | Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift |
| title_full | Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift |
| title_fullStr | Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift |
| title_full_unstemmed | Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift |
| title_short | Comparison of rabbit corneal changes during different preservation techniques using optisol-GS and airlift |
| title_sort | comparison of rabbit corneal changes during different preservation techniques using optisol gs and airlift |
| url | https://doi.org/10.1038/s41598-023-34039-5 |
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